Production of muconic acid in plants
Abstract
Muconic acid (MA) is a dicarboxylic acid used for the production of industrially relevant chemicals such as adipic acid, terephthalic acid, and caprolactam. Because the synthesis of these polymer precursors generates toxic intermediates by utilizing petroleum-derived chemicals and corrosive catalysts, the development of alternative strategies for the bio-based production of MA has garnered significant interest. Plants produce organic carbon skeletons by harvesting carbon dioxide and energy from the sun, and therefore represent advantageous hosts for engineered metabolic pathways towards the manufacturing of chemicals. In this work, we engineered Arabidopsis to demonstrate that plants can serve as green factories for the bio-manufacturing of MA. In particular, dual expression of plastid-targeted bacterial salicylate hydroxylase (NahG) and catechol 1,2-dioxygenase (CatA) resulted in the conversion of the endogenous salicylic acid (SA) pool into MA via catechol. Sequential increase of SA derived from the shikimate pathway was achieved by expressing plastid-targeted versions of bacterial salicylate synthase (Irp9) and feedback-resistant 3-deoxy-D-arabino-heptulosonate synthase (AroG). Introducing this SA over-producing strategy into engineered plants that co-express NahG and CatA resulted in a 50-fold increase in MA titers. Considering that MA was easily recovered from senesced plant biomass after harvest, we envision the phytoproduction of MA as a beneficial optionmore »
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1630142
- Alternate Identifier(s):
- OSTI ID: 1465705
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Metabolic Engineering
- Additional Journal Information:
- Journal Name: Metabolic Engineering Journal Volume: 46 Journal Issue: C; Journal ID: ISSN 1096-7176
- Publisher:
- Elsevier
- Country of Publication:
- Belgium
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Muconic acid; Salicylic acid; Catechol; Shikimate; Plastid; Arabidopsis
Citation Formats
Eudes, Aymerick, Berthomieu, Roland, Hao, Zhangying, Zhao, Nanxia, Benites, Veronica Teixeira, Baidoo, Edward E. K., and Loqué, Dominique. Production of muconic acid in plants. Belgium: N. p., 2018.
Web. doi:10.1016/j.ymben.2018.02.002.
Eudes, Aymerick, Berthomieu, Roland, Hao, Zhangying, Zhao, Nanxia, Benites, Veronica Teixeira, Baidoo, Edward E. K., & Loqué, Dominique. Production of muconic acid in plants. Belgium. https://doi.org/10.1016/j.ymben.2018.02.002
Eudes, Aymerick, Berthomieu, Roland, Hao, Zhangying, Zhao, Nanxia, Benites, Veronica Teixeira, Baidoo, Edward E. K., and Loqué, Dominique. Thu .
"Production of muconic acid in plants". Belgium. https://doi.org/10.1016/j.ymben.2018.02.002.
@article{osti_1630142,
title = {Production of muconic acid in plants},
author = {Eudes, Aymerick and Berthomieu, Roland and Hao, Zhangying and Zhao, Nanxia and Benites, Veronica Teixeira and Baidoo, Edward E. K. and Loqué, Dominique},
abstractNote = {Muconic acid (MA) is a dicarboxylic acid used for the production of industrially relevant chemicals such as adipic acid, terephthalic acid, and caprolactam. Because the synthesis of these polymer precursors generates toxic intermediates by utilizing petroleum-derived chemicals and corrosive catalysts, the development of alternative strategies for the bio-based production of MA has garnered significant interest. Plants produce organic carbon skeletons by harvesting carbon dioxide and energy from the sun, and therefore represent advantageous hosts for engineered metabolic pathways towards the manufacturing of chemicals. In this work, we engineered Arabidopsis to demonstrate that plants can serve as green factories for the bio-manufacturing of MA. In particular, dual expression of plastid-targeted bacterial salicylate hydroxylase (NahG) and catechol 1,2-dioxygenase (CatA) resulted in the conversion of the endogenous salicylic acid (SA) pool into MA via catechol. Sequential increase of SA derived from the shikimate pathway was achieved by expressing plastid-targeted versions of bacterial salicylate synthase (Irp9) and feedback-resistant 3-deoxy-D-arabino-heptulosonate synthase (AroG). Introducing this SA over-producing strategy into engineered plants that co-express NahG and CatA resulted in a 50-fold increase in MA titers. Considering that MA was easily recovered from senesced plant biomass after harvest, we envision the phytoproduction of MA as a beneficial option to add value to bioenergy crops.},
doi = {10.1016/j.ymben.2018.02.002},
journal = {Metabolic Engineering},
number = C,
volume = 46,
place = {Belgium},
year = {Thu Mar 01 00:00:00 EST 2018},
month = {Thu Mar 01 00:00:00 EST 2018}
}
https://doi.org/10.1016/j.ymben.2018.02.002
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