Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II
Abstract
The Photosystem II reaction center is vulnerable to photoinhibition. The D1 and D2 proteins, lying at the core of the photosystem, are susceptible to oxidative modification by reactive oxygen species that are formed by the photosystem during illumination. Using spin probes and EPR spectroscopy, we have determined that both O2•– and HO• are involved in the photoinhibitory process. Using tandem mass spectroscopy, we have identified a number of oxidatively modified D1 and D2 residues. Our analysis indicates that these oxidative modifications are associated with formation of HO• at both the Mn4O5Ca cluster and the nonheme iron. Additionally, O2•– appears to be formed by the reduction of O2 at either PheoD1 or QA. Early oxidation of D1:332H, which is coordinated with the Mn1 of the Mn4O5Ca cluster, appears to initiate a cascade of oxidative events that lead to the oxidative modification of numerous residues in the C termini of the D1 and D2 proteins on the donor side of the photosystem. Oxidation of D2:244Y, which is a bicarbonate ligand for the nonheme iron, induces the propagation of oxidative reactions in residues of the D-de loop of the D2 protein on the electron acceptor side of the photosystem. Finally, D1:130E and D2:246Mmore »
- Authors:
-
- Palacky Univ., Olomouc (Czech Republic)
- Louisiana State Univ., Baton Rouge, LA (United States)
- Univ. of Cincinnati, Cincinnati, OH (United States)
- Publication Date:
- Research Org.:
- Univ. of Cincinnati, Cincinnati, OH (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1465487
- Grant/Contract Number:
- FG02-09ER20310; FG02-98ER20310
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 114; Journal Issue: 11; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; photosynthesis; Photosystem II; reactive oxygen species; photo inhibition; mass spectrometry
Citation Formats
Kale, Ravindra, Hebert, Annette E., Frankel, Laurie K., Sallans, Larry, Bricker, Terry M., and Pospisil, Pavel. Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. United States: N. p., 2017.
Web. doi:10.1073/pnas.1618922114.
Kale, Ravindra, Hebert, Annette E., Frankel, Laurie K., Sallans, Larry, Bricker, Terry M., & Pospisil, Pavel. Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. United States. https://doi.org/10.1073/pnas.1618922114
Kale, Ravindra, Hebert, Annette E., Frankel, Laurie K., Sallans, Larry, Bricker, Terry M., and Pospisil, Pavel. Mon .
"Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II". United States. https://doi.org/10.1073/pnas.1618922114. https://www.osti.gov/servlets/purl/1465487.
@article{osti_1465487,
title = {Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II},
author = {Kale, Ravindra and Hebert, Annette E. and Frankel, Laurie K. and Sallans, Larry and Bricker, Terry M. and Pospisil, Pavel},
abstractNote = {The Photosystem II reaction center is vulnerable to photoinhibition. The D1 and D2 proteins, lying at the core of the photosystem, are susceptible to oxidative modification by reactive oxygen species that are formed by the photosystem during illumination. Using spin probes and EPR spectroscopy, we have determined that both O2•– and HO• are involved in the photoinhibitory process. Using tandem mass spectroscopy, we have identified a number of oxidatively modified D1 and D2 residues. Our analysis indicates that these oxidative modifications are associated with formation of HO• at both the Mn4O5Ca cluster and the nonheme iron. Additionally, O2•– appears to be formed by the reduction of O2 at either PheoD1 or QA. Early oxidation of D1:332H, which is coordinated with the Mn1 of the Mn4O5Ca cluster, appears to initiate a cascade of oxidative events that lead to the oxidative modification of numerous residues in the C termini of the D1 and D2 proteins on the donor side of the photosystem. Oxidation of D2:244Y, which is a bicarbonate ligand for the nonheme iron, induces the propagation of oxidative reactions in residues of the D-de loop of the D2 protein on the electron acceptor side of the photosystem. Finally, D1:130E and D2:246M are oxidatively modified by O2•– formed by the reduction of O2 either by PheoD1•– or QA•–. Furthermore, the identification of specific amino acid residues oxidized by reactive oxygen species provides insights into the mechanism of damage to the D1 and D2 proteins under light stress.},
doi = {10.1073/pnas.1618922114},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 11,
volume = 114,
place = {United States},
year = {Mon Mar 06 00:00:00 EST 2017},
month = {Mon Mar 06 00:00:00 EST 2017}
}
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