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Title: A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis

Yeast have long been known to possess a cell wall integrity (CWI) system, and recently an analogous systemhas been described for the primary walls of plants (PCWI) that leads to changes in plant growth and cell wall composition. A similar system has been proposed to exist for secondary cell walls (SCWI). However, there is little data to support this. Here, we analyzed the stem transcriptome of a set of cell wall biosynthetic mutants in order to investigate whether cell wall damage, in this case caused by aberrant xylan synthesis, activates a signaling cascade or changes in cell wall synthesis gene expression. Our data revealed remarkably few changes to the transcriptome. We hypothesize that this is because cells undergoing secondary cell wall thickening have entered a committed programme leading to cell death, and therefore a SCWI system would have limited impact. The absence of transcriptomic responses to secondary cell wall alterations may facilitate engineering of the secondary cell wall of plants.
Authors:
 [1] ;  [2] ;  [1]
  1. Univ. of Cambridge (United Kingdom). Dept. of Biohemistry
  2. Univ. of Cambridge (United Kingdom). Dept. of Biohemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 9; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1465453

Faria-Blanc, Nuno, Mortimer, Jenny C., and Dupree, Paul. A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis. United States: N. p., Web. doi:10.3389/fpls.2018.00384.
Faria-Blanc, Nuno, Mortimer, Jenny C., & Dupree, Paul. A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis. United States. doi:10.3389/fpls.2018.00384.
Faria-Blanc, Nuno, Mortimer, Jenny C., and Dupree, Paul. 2018. "A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis". United States. doi:10.3389/fpls.2018.00384. https://www.osti.gov/servlets/purl/1465453.
@article{osti_1465453,
title = {A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis},
author = {Faria-Blanc, Nuno and Mortimer, Jenny C. and Dupree, Paul},
abstractNote = {Yeast have long been known to possess a cell wall integrity (CWI) system, and recently an analogous systemhas been described for the primary walls of plants (PCWI) that leads to changes in plant growth and cell wall composition. A similar system has been proposed to exist for secondary cell walls (SCWI). However, there is little data to support this. Here, we analyzed the stem transcriptome of a set of cell wall biosynthetic mutants in order to investigate whether cell wall damage, in this case caused by aberrant xylan synthesis, activates a signaling cascade or changes in cell wall synthesis gene expression. Our data revealed remarkably few changes to the transcriptome. We hypothesize that this is because cells undergoing secondary cell wall thickening have entered a committed programme leading to cell death, and therefore a SCWI system would have limited impact. The absence of transcriptomic responses to secondary cell wall alterations may facilitate engineering of the secondary cell wall of plants.},
doi = {10.3389/fpls.2018.00384},
journal = {Frontiers in Plant Science},
number = ,
volume = 9,
place = {United States},
year = {2018},
month = {3}
}

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