Upconverting nanoparticle micro-lightbulbs designed for deep tissue optical stimulation and imaging
Abstract
Optical methods for imaging and stimulation of biological events based on the use of visible light are limited to the superficial layers of tissue due to the significant absorption and scattering of light. Here, we demonstrate the design and implementation of passive micro-structured lightbulbs (MLBs) containing bright-emitting lanthanide-doped upconverting nanoparticles (UCNPs) for light delivery deep into the tissue. The MLBs are realized as cylindrical pillars made of Parylene C polymer that can be implanted deep into the tissue. The encapsulated UCNPs absorb near-infrared (NIR) light at λ = 980 nm, which undergoes much less absorption than the blue light in the brain tissue, and then locally emit blue light (1G4→3H6 and 1D2→3F4 transitions) that can be used for optogenetic excitation of neurons in the brain. The 3H4→3H6 transition will result in the emission of higher energy NIR photons at λ = 800 nm that can be used for imaging and tracking MLBs through thick tissue.
- Authors:
- Publication Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); Ministry of Science and ICT, South Korea
- OSTI Identifier:
- 1465251
- Alternate Identifier(s):
- OSTI ID: 1474841; OSTI ID: 1477370
- Report Number(s):
- NREL/JA-6A60-72480
Journal ID: ISSN 2156-7085
- Grant/Contract Number:
- AC02-05CH11231; AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Biomedical Optics Express
- Additional Journal Information:
- Journal Name: Biomedical Optics Express Journal Volume: 9 Journal Issue: 9; Journal ID: ISSN 2156-7085
- Publisher:
- Optical Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY; infrared devices; light; nanoparticles; polymeric implants
Citation Formats
Chamanzar, Maysamreza, Garfield, David J., Iafrati, Jillian, Chan, Emory M., Sohal, Vikaas, Cohen, Bruce E., Schuck, P. James, and Maharbiz, Michel M.. Upconverting nanoparticle micro-lightbulbs designed for deep tissue optical stimulation and imaging. United States: N. p., 2018.
Web. doi:10.1364/BOE.9.004359.
Chamanzar, Maysamreza, Garfield, David J., Iafrati, Jillian, Chan, Emory M., Sohal, Vikaas, Cohen, Bruce E., Schuck, P. James, & Maharbiz, Michel M.. Upconverting nanoparticle micro-lightbulbs designed for deep tissue optical stimulation and imaging. United States. https://doi.org/10.1364/BOE.9.004359
Chamanzar, Maysamreza, Garfield, David J., Iafrati, Jillian, Chan, Emory M., Sohal, Vikaas, Cohen, Bruce E., Schuck, P. James, and Maharbiz, Michel M.. Mon .
"Upconverting nanoparticle micro-lightbulbs designed for deep tissue optical stimulation and imaging". United States. https://doi.org/10.1364/BOE.9.004359.
@article{osti_1465251,
title = {Upconverting nanoparticle micro-lightbulbs designed for deep tissue optical stimulation and imaging},
author = {Chamanzar, Maysamreza and Garfield, David J. and Iafrati, Jillian and Chan, Emory M. and Sohal, Vikaas and Cohen, Bruce E. and Schuck, P. James and Maharbiz, Michel M.},
abstractNote = {Optical methods for imaging and stimulation of biological events based on the use of visible light are limited to the superficial layers of tissue due to the significant absorption and scattering of light. Here, we demonstrate the design and implementation of passive micro-structured lightbulbs (MLBs) containing bright-emitting lanthanide-doped upconverting nanoparticles (UCNPs) for light delivery deep into the tissue. The MLBs are realized as cylindrical pillars made of Parylene C polymer that can be implanted deep into the tissue. The encapsulated UCNPs absorb near-infrared (NIR) light at λ = 980 nm, which undergoes much less absorption than the blue light in the brain tissue, and then locally emit blue light (1G4→3H6 and 1D2→3F4 transitions) that can be used for optogenetic excitation of neurons in the brain. The 3H4→3H6 transition will result in the emission of higher energy NIR photons at λ = 800 nm that can be used for imaging and tracking MLBs through thick tissue.},
doi = {10.1364/BOE.9.004359},
journal = {Biomedical Optics Express},
number = 9,
volume = 9,
place = {United States},
year = {Mon Aug 20 00:00:00 EDT 2018},
month = {Mon Aug 20 00:00:00 EDT 2018}
}
https://doi.org/10.1364/BOE.9.004359
Web of Science
Works referenced in this record:
Imaging Brain Activity With Voltage- and Calcium-Sensitive Dyes
journal, April 2005
- Baker, Bradley J.; Kosmidis, Efstratios K.; Vucinic, Dejan
- Cellular and Molecular Neurobiology, Vol. 25, Issue 2
Upconverting nanoparticles: a versatile platform for wide-field two-photon microscopy and multi-modal in vivo imaging
journal, January 2015
- Park, Yong Il; Lee, Kang Taek; Suh, Yung Doug
- Chemical Society Reviews, Vol. 44, Issue 6
Single-nanocrystal sensitivity achieved by enhanced upconversion luminescence
journal, September 2013
- Zhao, Jiangbo; Jin, Dayong; Schartner, Erik P.
- Nature Nanotechnology, Vol. 8, Issue 10
Multiwaveguide implantable probe for light delivery to sets of distributed brain targets
journal, January 2010
- Zorzos, Anthony N.; Boyden, Edward S.; Fonstad, Clifton G.
- Optics Letters, Vol. 35, Issue 24
Energy-Looping Nanoparticles: Harnessing Excited-State Absorption for Deep-Tissue Imaging
journal, September 2016
- Levy, Elizabeth S.; Tajon, Cheryl A.; Bischof, Thomas S.
- ACS Nano, Vol. 10, Issue 9
Engineering bright sub-10-nm upconverting nanocrystals for single-molecule imaging
journal, March 2014
- Gargas, Daniel J.; Chan, Emory M.; Ostrowski, Alexis D.
- Nature Nanotechnology, Vol. 9, Issue 4
Continuous-wave upconverting nanoparticle microlasers
journal, June 2018
- Fernandez-Bravo, Angel; Yao, Kaiyuan; Barnard, Edward S.
- Nature Nanotechnology, Vol. 13, Issue 7
Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications
journal, December 2015
- Wu, Xiang; Zhang, Yuanwei; Takle, Kendra
- ACS Nano, Vol. 10, Issue 1
Trapping red blood cells in living animals using optical tweezers
journal, April 2013
- Zhong, Min-Cheng; Wei, Xun-Bin; Zhou, Jin-Hua
- Nature Communications, Vol. 4, Issue 1
An implantable neural probe with monolithically integrated dielectric waveguide and recording electrodes for optogenetics applications
journal, August 2013
- Wu, Fan; Stark, Eran; Im, Maesoon
- Journal of Neural Engineering, Vol. 10, Issue 5
Optogenetic Brain Interfaces
journal, January 2014
- Pashaie, Ramin; Anikeeva, Polina
- IEEE Reviews in Biomedical Engineering, Vol. 7
In vivo three-photon microscopy of subcortical structures within an intact mouse brain
journal, January 2013
- Horton, Nicholas G.; Wang, Ke; Kobat, Demirhan
- Nature Photonics, Vol. 7, Issue 3
24.1 A miniaturized 64-channel 225μW wireless electrocorticographic neural sensor
conference, February 2014
- Muller, Rikky; Le, Hanh-Phuc; Li, Wen
- 2014 IEEE International Solid- State Circuits Conference (ISSCC), 2014 IEEE International Solid-State Circuits Conference Digest of Technical Papers (ISSCC)
Reproducible, High-Throughput Synthesis of Colloidal Nanocrystals for Optimization in Multidimensional Parameter Space
journal, May 2010
- Chan, Emory M.; Xu, Chenxu; Mao, Alvin W.
- Nano Letters, Vol. 10, Issue 5
Direct Evidence for Coupled Surface and Concentration Quenching Dynamics in Lanthanide-Doped Nanocrystals
journal, February 2017
- Johnson, Noah J. J.; He, Sha; Diao, Shuo
- Journal of the American Chemical Society, Vol. 139, Issue 8
Controlled Synthesis and Single-Particle Imaging of Bright, Sub-10 nm Lanthanide-Doped Upconverting Nanocrystals
journal, February 2012
- Ostrowski, Alexis D.; Chan, Emory M.; Gargas, Daniel J.
- ACS Nano, Vol. 6, Issue 3
In vivo two-photon microscopy to 1.6-mm depth in mouse cortex
journal, January 2011
- Kobat, Demirhan; Horton, Nicholas G.; Xu, Chris
- Journal of Biomedical Optics, Vol. 16, Issue 10
Three-dimensional multiwaveguide probe array for light delivery to distributed brain circuits
journal, January 2012
- Zorzos, Anthony N.; Scholvin, Jorg; Boyden, Edward S.
- Optics Letters, Vol. 37, Issue 23
A 3D glass optrode array for optical neural stimulation
journal, January 2012
- Abaya, T. V. F.; Blair, S.; Tathireddy, P.
- Biomedical Optics Express, Vol. 3, Issue 12
Revised Kubelka–Munk theory III A general theory of light propagation in scattering and absorptive media
journal, January 2005
- Yang, Li; Miklavcic, Stanley J.
- Journal of the Optical Society of America A, Vol. 22, Issue 9
Ultra-high Q planar silicon microdisk resonators for chip-scale silicon photonics
journal, January 2007
- Soltani, Mohammad; Yegnanarayanan, Siva; Adibi, Ali
- Optics Express, Vol. 15, Issue 8
Theory of free space coupling to high-Q whispering gallery modes
journal, January 2013
- Zou, Chang-Ling; Shu, Fang-Jie; Sun, Fang-Wen
- Optics Express, Vol. 21, Issue 8
A history of optogenetics: the development of tools for controlling brain circuits with light
journal, May 2011
- Boyden, Edward
- F1000 Biology Reports, Vol. 3
Interfacing whispering-gallery microresonators and free space light with cavity enhanced Rayleigh scattering
journal, September 2014
- Zhu, Jiangang; Özdemir, Şahin K.; Yilmaz, Huzeyfe
- Scientific Reports, Vol. 4, Issue 1