Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface
Abstract
Here, the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. In conclusion, this immediate drop on demand technology/open port sampling interface/ESI–MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.
- Authors:
-
[1];
[1];
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Dispendix GmbH, Stuttgart (Germany)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1465070
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioanalysis
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 21; Journal ID: ISSN 1757-6180
- Publisher:
- Future Science Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION; drop dispensing; electrospray ionization; immediate drop on demand; mass spectrometry; microtiter plates; noncontact dispensing; open port sampling interface
Citation Formats
Van Berkel, Gary J., Kertesz, Vilmos, and Boeltz, Harry. Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface. United States: N. p., 2017.
Web. doi:10.4155/bio-2017-0104.
Van Berkel, Gary J., Kertesz, Vilmos, & Boeltz, Harry. Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface. United States. https://doi.org/10.4155/bio-2017-0104
Van Berkel, Gary J., Kertesz, Vilmos, and Boeltz, Harry. Thu .
"Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface". United States. https://doi.org/10.4155/bio-2017-0104. https://www.osti.gov/servlets/purl/1465070.
@article{osti_1465070,
title = {Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling interface},
author = {Van Berkel, Gary J. and Kertesz, Vilmos and Boeltz, Harry},
abstractNote = {Here, the immediate drop on demand technology to a mass spectrometer via the recently introduced open port sampling interface and ESI. A maximum sample analysis throughput of 5 s per sample was demonstrated. Signal reproducibility was 10% or better as demonstrated by the quantitative analysis of propranolol and its stable isotope-labeled internal standard propranolol-d7. The ability of the system to multiply charge and analyze macromolecules was demonstrated using the protein cytochrome c. In conclusion, this immediate drop on demand technology/open port sampling interface/ESI–MS combination allowed for the quantitative analysis of relatively small mass analytes and was used for the identification of macromolecules like proteins.},
doi = {10.4155/bio-2017-0104},
journal = {Bioanalysis},
number = 21,
volume = 9,
place = {United States},
year = {Thu Nov 02 00:00:00 EDT 2017},
month = {Thu Nov 02 00:00:00 EDT 2017}
}
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