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Title: Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes

Abstract

The unique properties of chlorosomes, arising out of the self-assembled bateriochlorophyll (BChl) c structure, have made them attractive for use in solar cells. In this work, we have demonstrated the self-assembly of BChl c in aerosolized droplets to mimic naturally occurring chlorosomes. We compare two different methods for self-assembly of BChl c, one using a single-solvent and the other using two-solvents, and demonstrate the superiority of the two-solvent method. Results show that the self-assembled BChl c sprayed at different concentrations resulted in a varying red shift of 69-75 nm in absorption spectrum compared to the solution, which has peak at 668 nm corresponding to the monomeric BChl c. The sample fluoresces at 780 nm indicating a quality of self-assembly comparable to that observed in naturally occurring chlorosomes. In order to mimic chlorosomes, solution containing BChl c, BChl a, lipids and carotenes in same proportion as in chlorosomes is sprayed. The resulting self-assembly has an absorption peak at 750 nm, shifted by 82 nm compared to that of monomers and the fluorescence peak at 790 nm. Thus in presence of lipids and carotenes, both the absorption and fluorescence peaks are red shifted. Further, using grazing incidence small angle X-ray scattering (GISAXS),more » we characterized the deposited films, and the 2D X-ray scattering patterns of sample clearly indicate the distinct lamellar structure as present in chlorosomes. Here, the results of this work provide new insights into self-assembly in aerosolized droplets, which can be used for assembling a wide range of molecules.« less

Authors:
 [1];  [2];  [1];  [1];  [3];  [1];  [4];  [1];  [1]
  1. Washington Univ. in St. Louis, St. Louis, MO (United States)
  2. Washington Univ. in St. Louis, St. Louis, MO (United States); Johns Hopkins Univ., Baltimore, MD (United States)
  3. Washington Univ. in St. Louis, St. Louis, MO (United States); Univ. of California, Berkeley, CA (United States)
  4. Argonne National Lab. (ANL), Lemont, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1464756
Alternate Identifier(s):
OSTI ID: 1582781
Grant/Contract Number:  
AC02-06CH11357; SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Photochemistry and Photobiology B: Biology
Additional Journal Information:
Journal Volume: 185; Journal Issue: C; Journal ID: ISSN 1011-1344
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacteriochlorophyll c; chlorosome; electrospray atomization; self-assembly

Citation Formats

Shah, Vivek B., Ferris, Chloe, Orf, Gregory S., Kavadiya, Shalinee, Ray, Jessica R., Jun, Young -Shin, Lee, Byeongdu, Blankenship, Robert E., and Biswas, Pratim. Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes. United States: N. p., 2018. Web. doi:10.1016/j.jphotobiol.2018.04.032.
Shah, Vivek B., Ferris, Chloe, Orf, Gregory S., Kavadiya, Shalinee, Ray, Jessica R., Jun, Young -Shin, Lee, Byeongdu, Blankenship, Robert E., & Biswas, Pratim. Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes. United States. https://doi.org/10.1016/j.jphotobiol.2018.04.032
Shah, Vivek B., Ferris, Chloe, Orf, Gregory S., Kavadiya, Shalinee, Ray, Jessica R., Jun, Young -Shin, Lee, Byeongdu, Blankenship, Robert E., and Biswas, Pratim. Thu . "Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes". United States. https://doi.org/10.1016/j.jphotobiol.2018.04.032. https://www.osti.gov/servlets/purl/1464756.
@article{osti_1464756,
title = {Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes},
author = {Shah, Vivek B. and Ferris, Chloe and Orf, Gregory S. and Kavadiya, Shalinee and Ray, Jessica R. and Jun, Young -Shin and Lee, Byeongdu and Blankenship, Robert E. and Biswas, Pratim},
abstractNote = {The unique properties of chlorosomes, arising out of the self-assembled bateriochlorophyll (BChl) c structure, have made them attractive for use in solar cells. In this work, we have demonstrated the self-assembly of BChl c in aerosolized droplets to mimic naturally occurring chlorosomes. We compare two different methods for self-assembly of BChl c, one using a single-solvent and the other using two-solvents, and demonstrate the superiority of the two-solvent method. Results show that the self-assembled BChl c sprayed at different concentrations resulted in a varying red shift of 69-75 nm in absorption spectrum compared to the solution, which has peak at 668 nm corresponding to the monomeric BChl c. The sample fluoresces at 780 nm indicating a quality of self-assembly comparable to that observed in naturally occurring chlorosomes. In order to mimic chlorosomes, solution containing BChl c, BChl a, lipids and carotenes in same proportion as in chlorosomes is sprayed. The resulting self-assembly has an absorption peak at 750 nm, shifted by 82 nm compared to that of monomers and the fluorescence peak at 790 nm. Thus in presence of lipids and carotenes, both the absorption and fluorescence peaks are red shifted. Further, using grazing incidence small angle X-ray scattering (GISAXS), we characterized the deposited films, and the 2D X-ray scattering patterns of sample clearly indicate the distinct lamellar structure as present in chlorosomes. Here, the results of this work provide new insights into self-assembly in aerosolized droplets, which can be used for assembling a wide range of molecules.},
doi = {10.1016/j.jphotobiol.2018.04.032},
journal = {Journal of Photochemistry and Photobiology B: Biology},
number = C,
volume = 185,
place = {United States},
year = {2018},
month = {4}
}

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Works referencing / citing this record:

Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene
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