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Title: Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes

Abstract

The unique properties of chlorosomes, arising out of the self-assembled bateriochlorophyll (BChl) c structure, have made them attractive for use in solar cells. In this work, we have demonstrated the self-assembly of BChl c in aerosolized droplets to mimic naturally occurring chlorosomes. We compare two different methods for self-assembly of BChl c, one using a single-solvent and the other using two-solvents, and demonstrate the superiority of the two-solvent method. Results show that the self-assembled BChl c sprayed at different concentrations resulted in a varying red shift of 69-75 nm in absorption spectrum compared to the solution, which has peak at 668 nm corresponding to the monomeric BChl c. The sample fluoresces at 780 nm indicating a quality of self-assembly comparable to that observed in naturally occurring chlorosomes. In order to mimic chlorosomes, solution containing BChl c, BChl a, lipids and carotenes in same proportion as in chlorosomes is sprayed. The resulting self-assembly has an absorption peak at 750 nm, shifted by 82 nm compared to that of monomers and the fluorescence peak at 790 nm. Thus in presence of lipids and carotenes, both the absorption and fluorescence peaks are red shifted. Further, using grazing incidence small angle X-ray scattering (GISAXS),more » we characterized the deposited films, and the 2D X-ray scattering patterns of sample clearly indicate the distinct lamellar structure as present in chlorosomes. Here, the results of this work provide new insights into self-assembly in aerosolized droplets, which can be used for assembling a wide range of molecules.« less

Authors:
 [1];  [2];  [1];  [1];  [3];  [1];  [4];  [1];  [1]
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  1. Washington Univ. in St. Louis, St. Louis, MO (United States)
  2. Washington Univ. in St. Louis, St. Louis, MO (United States); Johns Hopkins Univ., Baltimore, MD (United States)
  3. Washington Univ. in St. Louis, St. Louis, MO (United States); Univ. of California, Berkeley, CA (United States)
  4. Argonne National Lab. (ANL), Lemont, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1464756
Alternate Identifier(s):
OSTI ID: 1582781
Grant/Contract Number:  
AC02-06CH11357; SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Photochemistry and Photobiology B: Biology
Additional Journal Information:
Journal Volume: 185; Journal Issue: C; Journal ID: ISSN 1011-1344
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacteriochlorophyll c; chlorosome; electrospray atomization; self-assembly

Citation Formats

Shah, Vivek B., Ferris, Chloe, Orf, Gregory S., Kavadiya, Shalinee, Ray, Jessica R., Jun, Young -Shin, Lee, Byeongdu, Blankenship, Robert E., and Biswas, Pratim. Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes. United States: N. p., 2018. Web. doi:10.1016/j.jphotobiol.2018.04.032.
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Shah, Vivek B., Ferris, Chloe, Orf, Gregory S., Kavadiya, Shalinee, Ray, Jessica R., Jun, Young -Shin, Lee, Byeongdu, Blankenship, Robert E., & Biswas, Pratim. Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes. United States. https://doi.org/10.1016/j.jphotobiol.2018.04.032
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Shah, Vivek B., Ferris, Chloe, Orf, Gregory S., Kavadiya, Shalinee, Ray, Jessica R., Jun, Young -Shin, Lee, Byeongdu, Blankenship, Robert E., and Biswas, Pratim. Thu . "Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes". United States. https://doi.org/10.1016/j.jphotobiol.2018.04.032. https://www.osti.gov/servlets/purl/1464756.
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@article{osti_1464756,
title = {Supramolecular self-assembly of bacteriochlorophyll c molecules in aerosolized droplets to synthesize biomimetic chlorosomes},
author = {Shah, Vivek B. and Ferris, Chloe and Orf, Gregory S. and Kavadiya, Shalinee and Ray, Jessica R. and Jun, Young -Shin and Lee, Byeongdu and Blankenship, Robert E. and Biswas, Pratim},
abstractNote = {The unique properties of chlorosomes, arising out of the self-assembled bateriochlorophyll (BChl) c structure, have made them attractive for use in solar cells. In this work, we have demonstrated the self-assembly of BChl c in aerosolized droplets to mimic naturally occurring chlorosomes. We compare two different methods for self-assembly of BChl c, one using a single-solvent and the other using two-solvents, and demonstrate the superiority of the two-solvent method. Results show that the self-assembled BChl c sprayed at different concentrations resulted in a varying red shift of 69-75 nm in absorption spectrum compared to the solution, which has peak at 668 nm corresponding to the monomeric BChl c. The sample fluoresces at 780 nm indicating a quality of self-assembly comparable to that observed in naturally occurring chlorosomes. In order to mimic chlorosomes, solution containing BChl c, BChl a, lipids and carotenes in same proportion as in chlorosomes is sprayed. The resulting self-assembly has an absorption peak at 750 nm, shifted by 82 nm compared to that of monomers and the fluorescence peak at 790 nm. Thus in presence of lipids and carotenes, both the absorption and fluorescence peaks are red shifted. Further, using grazing incidence small angle X-ray scattering (GISAXS), we characterized the deposited films, and the 2D X-ray scattering patterns of sample clearly indicate the distinct lamellar structure as present in chlorosomes. Here, the results of this work provide new insights into self-assembly in aerosolized droplets, which can be used for assembling a wide range of molecules.},
doi = {10.1016/j.jphotobiol.2018.04.032},
journal = {Journal of Photochemistry and Photobiology B: Biology},
number = C,
volume = 185,
place = {United States},
year = {2018},
month = {4}
}
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Works referenced in this record:

Chlorosome antenna complexes from green photosynthetic bacteria
journal, June 2013

Electrospray-assisted characterization and deposition of chlorosomes to fabricate a biomimetic light-harvesting device
journal, January 2010

  • Modesto-Lopez, Luis B.; Thimsen, Elijah J.; Collins, Aaron M.
  • Energy Environ. Sci., Vol. 3, Issue 2, p. 216-222
  • DOI: 10.1039/B914758F

Linker-Free Deposition and Adhesion of Photosystem I onto Nanostructured TiO 2 for Biohybrid Photoelectrochemical Cells
journal, January 2015

  • Shah, Vivek B.; Henson, William R.; Chadha, Tandeep S.
  • Langmuir, Vol. 31, Issue 5
  • DOI: 10.1021/la503776b

On the way to biomimetic dye aggregate solar cells
journal, January 2011

  • Marek, Peter L.; Hahn, Horst; Balaban, Teodor Silviu
  • Energy & Environmental Science, Vol. 4, Issue 7
  • DOI: 10.1039/c1ee01053k

Hierarchal Light-Harvesting Aggregates and Their Potential for Solar Energy Applications
journal, February 2012

  • McHale, Jeanne L.
  • The Journal of Physical Chemistry Letters, Vol. 3, Issue 5
  • DOI: 10.1021/jz3000678

Multifunctional Nanoparticles Self-Assembled from Small Organic Building Blocks for Biomedicine
journal, June 2016

Giant light-harvesting nanoantenna for single-molecule detection in ambient light
journal, September 2017

Biological Applications of Supramolecular Assemblies Designed for Excitation Energy Transfer
journal, June 2015

  • Peng, Hui-Qing; Niu, Li-Ya; Chen, Yu-Zhe
  • Chemical Reviews, Vol. 115, Issue 15
  • DOI: 10.1021/cr5007057

Light-Harvesting Systems Based on Organic Nanocrystals To Mimic Chlorosomes
journal, January 2016

  • Chen, Peng-Zhong; Weng, Yu-Xiang; Niu, Li-Ya
  • Angewandte Chemie International Edition, Vol. 55, Issue 8
  • DOI: 10.1002/anie.201510503

Anisotropic Organization and Microscopic Manipulation of Self-Assembling Synthetic Porphyrin Microrods That Mimic Chlorosomes: Bacterial Light-Harvesting Systems
journal, December 2011

  • Chappaz-Gillot, Cyril; Marek, Peter L.; Blaive, Bruno J.
  • Journal of the American Chemical Society, Vol. 134, Issue 2
  • DOI: 10.1021/ja203838p

Autocatalyzed Self-Aggregation of (3 R )-[Et,Et]Bacteriochlorophyll c F Molecules in Nonpolar Solvents. Analysis of the Kinetics
journal, January 2000

  • Balaban, Teodor Silviu; Leitich, Johannes; Holzwarth, Alfred R.
  • The Journal of Physical Chemistry B, Vol. 104, Issue 6
  • DOI: 10.1021/jp992338p

Redox effects on the excited-state lifetime in chlorosomes and bacteriochlorophyll c oligomers
journal, January 1997

Self-aggregates of natural chlorophylls and their synthetic analogues in aqueous media for making light-harvesting systems
journal, November 2010

Scrambled Self-Assembly of Bacteriochlorophylls c and e in Aqueous Triton X-100 Micelles
journal, December 2013

  • Saga, Yoshitaka; Saiki, Tatsuya; Takahashi, Naoya
  • Photochemistry and Photobiology, Vol. 90, Issue 3
  • DOI: 10.1111/php.12219

Polymer–Chlorosome Nanocomposites Consisting of Non-Native Combinations of Self-Assembling Bacteriochlorophylls
journal, June 2017

Aerosolized Droplet Mediated Self-Assembly of Photosynthetic Pigment Analogues and Deposition onto Substrates
journal, January 2014

A new bacteriochlorophyll a-protein complex associated with chlorosomes of green sulfur bacteria
journal, January 1986

De novo synthesis and properties of analogues of the self-assembling chlorosomal bacteriochlorophylls
journal, January 2011

  • Mass, Olga; Pandithavidana, Dinesh R.; Ptaszek, Marcin
  • New Journal of Chemistry, Vol. 35, Issue 11
  • DOI: 10.1039/c1nj20611g

Isolation and characterization of cytoplasmic membranes and chlorosomes from the green bacterium Chloroflexus aurantiacus.
journal, January 1982

Electrospraying of conducting liquids for monodisperse aerosol generation in the 4 nm to 1.8 μm diameter range
journal, September 1995

  • Chen, Da-Ren; Pui, David Y. H.; Kaufman, Stanley L.
  • Journal of Aerosol Science, Vol. 26, Issue 6, p. 963-977
  • DOI: 10.1016/0021-8502(95)00027-A

Electrospray of ionic precursor solutions to synthesize iron oxide nanoparticles: Modified scaling law
journal, February 2007

Effect of Carotenoid Biosynthesis Inhibition on the Chlorosome Organization in Chlorobium phaeobacteroides Strain CL1401
journal, June 2000

Characterization and deposition of various light-harvesting antenna complexes by electrospray atomization
journal, September 2012

  • Shah, Vivek B.; Orf, Gregory S.; Reisch, Sean
  • Analytical and Bioanalytical Chemistry, Vol. 404, Issue 8
  • DOI: 10.1007/s00216-012-6368-x

Structural Analysis of Block Copolymer Thin Films with Grazing Incidence Small-Angle X-ray Scattering
journal, May 2005

  • Lee, Byeongdu; Park, Insun; Yoon, Jinhwan
  • Macromolecules, Vol. 38, Issue 10
  • DOI: 10.1021/ma047562d

Multiple-scaled polymer surfaces investigated with micro-focus grazing-incidence small-angle X-ray scattering
journal, March 2003

  • Müller-Buschbaum, P.; Roth, S. V.; Burghammer, M.
  • Europhysics Letters (EPL), Vol. 61, Issue 5
  • DOI: 10.1209/epl/i2003-00119-4

Alternating syn-anti bacteriochlorophylls form concentric helical nanotubes in chlorosomes
journal, May 2009

  • Ganapathy, S.; Oostergetel, G. T.; Wawrzyniak, P. K.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 21
  • DOI: 10.1073/pnas.0903534106

Lamellar Organization of Pigments in Chlorosomes, the Light Harvesting Complexes of Green Photosynthetic Bacteria
journal, August 2004

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