Immobilisation of Platinum by Cupriavidus metallidurans
Abstract
The metal resistant bacterium Cupriavidus metallidurans CH34, challenged with aqueous platinous and platinic chloride, rapidly immobilized platinum. XANES/EXAFS analysis of these reaction systems demonstrated that platinum binding shifted from chloride to carboxyl functional groups within the bacteria. Pt(IV) was more toxic than Pt(II), presumably due to the oxidative stress imparted by the platinic form. Platinum immobilisation increased with time and with increasing concentrations of platinum. From a bacterial perspective, intracellular platinum concentrations were two to three orders of magnitude greater than the fluid phase, and became saturated at almost molar concentrations in both reaction systems. TEM revealed that C. metallidurans was also able to precipitate nm-scale colloidal platinum, primarily along the cell envelope where energy generation/electron transport occurs. Cells enriched in platinum shed outer membrane vesicles that were enriched in metallic, colloidal platinum, likely representing an important detoxification strategy. Furthermore, the formation of organo-platinum compounds and membrane encapsulated nanophase platinum, supports a role for bacteria in the formation and transport of platinum in natural systems, forming dispersion halos important to metal exploration.
- Authors:
-
- The Univ. of Western Ontario, London, ON (Canada)
- Canadian Light Source, Saskatoon, SK (Canada)
- The Univ. of Adelaide, Adelaide, SA (Australia); CSIRO Land and Water, Glen Osmond, SA (Australia)
- Pacific Northwest Consortium Synchrotron Radiation Facility, Argonne, IL (United States)
- The Univ. of Queensland, St. Lucia (Australia)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- Australian Research Council; Natural Sciences and Engineering Research Council of Canada (NSERC); USDOE
- OSTI Identifier:
- 1464755
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Minerals
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2075-163X
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Cupriavidus metallidurans; biomineralisation; geomicrobiology; platinum; synchrotron
Citation Formats
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., and Southam, Gordon. Immobilisation of Platinum by Cupriavidus metallidurans. United States: N. p., 2018.
Web. doi:10.3390/min8010010.
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., & Southam, Gordon. Immobilisation of Platinum by Cupriavidus metallidurans. United States. doi:10.3390/min8010010.
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., and Southam, Gordon. Fri .
"Immobilisation of Platinum by Cupriavidus metallidurans". United States. doi:10.3390/min8010010. https://www.osti.gov/servlets/purl/1464755.
@article{osti_1464755,
title = {Immobilisation of Platinum by Cupriavidus metallidurans},
author = {Campbell, Gordon and MacLean, Lachlan and Reith, Frank and Brewe, Dale and Gordon, Robert A. and Southam, Gordon},
abstractNote = {The metal resistant bacterium Cupriavidus metallidurans CH34, challenged with aqueous platinous and platinic chloride, rapidly immobilized platinum. XANES/EXAFS analysis of these reaction systems demonstrated that platinum binding shifted from chloride to carboxyl functional groups within the bacteria. Pt(IV) was more toxic than Pt(II), presumably due to the oxidative stress imparted by the platinic form. Platinum immobilisation increased with time and with increasing concentrations of platinum. From a bacterial perspective, intracellular platinum concentrations were two to three orders of magnitude greater than the fluid phase, and became saturated at almost molar concentrations in both reaction systems. TEM revealed that C. metallidurans was also able to precipitate nm-scale colloidal platinum, primarily along the cell envelope where energy generation/electron transport occurs. Cells enriched in platinum shed outer membrane vesicles that were enriched in metallic, colloidal platinum, likely representing an important detoxification strategy. Furthermore, the formation of organo-platinum compounds and membrane encapsulated nanophase platinum, supports a role for bacteria in the formation and transport of platinum in natural systems, forming dispersion halos important to metal exploration.},
doi = {10.3390/min8010010},
journal = {Minerals},
number = 1,
volume = 8,
place = {United States},
year = {2018},
month = {1}
}
Web of Science
Works referenced in this record:
The Geomicrobiology of Ore Deposits
journal, September 2005
- Southam, G.; Saunders, J. A.
- Economic Geology, Vol. 100, Issue 6
Bioreductive deposition of platinum nanoparticles on the bacterium Shewanella algae
journal, February 2007
- Konishi, Yasuhiro; Ohno, Kaori; Saitoh, Norizoh
- Journal of Biotechnology, Vol. 128, Issue 3
ATHENA , ARTEMIS , HEPHAESTUS : data analysis for X-ray absorption spectroscopy using IFEFFIT
journal, June 2005
- Ravel, B.; Newville, M.
- Journal of Synchrotron Radiation, Vol. 12, Issue 4
The Complete Genome Sequence of Cupriavidus metallidurans Strain CH34, a Master Survivalist in Harsh and Anthropogenic Environments
journal, May 2010
- Janssen, Paul J.; Van Houdt, Rob; Moors, Hugo
- PLoS ONE, Vol. 5, Issue 5
Electron-density distribution in crystals of potassium tetrachloroplatinate(II) and influence of X-ray diffuse scattering
journal, February 1983
- Ohba, S.; Sato, S.; Saito, Y.
- Acta Crystallographica Section B Structural Science, Vol. 39, Issue 1
Structural and Chemical Characterization of Placer Gold Grains: Implications for Bacterial Contributions to Grain Formation
journal, December 2014
- Shuster, Jeremiah; Johnston, Chad W.; Magarvey, Nathan A.
- Geomicrobiology Journal, Vol. 32, Issue 2
Morphology of Witwatersrand gold grains from the Basal Reef; evidence for their detrital origin
journal, April 1993
- Minter, W. E. L.; Goedhart, M.; Knight, J.
- Economic Geology, Vol. 88, Issue 2
The Role of Bacteria in the Supergene Environment of the Morenci Porphyry Copper Deposit, Greenlee County, Arizona
journal, January 2006
- Enders, M. S.; Knickerbocker, C.; Titley, S. R.
- Economic Geology, Vol. 101, Issue 1
Gold biomineralization by a metallophore from a gold-associated microbe
journal, February 2013
- Johnston, Chad W.; Wyatt, Morgan A.; Li, Xiang
- Nature Chemical Biology, Vol. 9, Issue 4
Biomineralization of Gold: Biofilms on Bacterioform Gold
journal, July 2006
- Reith, F.
- Science, Vol. 313, Issue 5784
Platinum in Earth surface environments
journal, April 2014
- Reith, F.; Campbell, S. G.; Ball, A. S.
- Earth-Science Reviews, Vol. 131
The in-vitro “growth” of gold grains
journal, January 2015
- Shuster, Jeremiah; Southam, Gordon
- Geology, Vol. 43, Issue 1
Ore Deposits of the Platinum-Group Elements
journal, August 2008
- Mungall, J. E.; Naldrett, A. J.
- Elements, Vol. 4, Issue 4
Bacterial resistances to toxic metal ions - a review
journal, January 1996
- Silver, Simon
- Gene, Vol. 179, Issue 1
An experimental study of Au removal from solution by non-metabolizing bacterial cells and their exudates
journal, June 2012
- Kenney, Janice P. L.; Song, Zhen; Bunker, Bruce A.
- Geochimica et Cosmochimica Acta, Vol. 87
Complexation of platinum, palladium and rhodium with inorganic ligands in the environment
journal, January 2008
- Colombo, C.; Oates, C. J.; Monhemius, A. J.
- Geochemistry: Exploration, Environment, Analysis, Vol. 8, Issue 1
The development of platinum-group minerals in laterites
journal, August 1986
- Bowles, John F. W.
- Economic Geology, Vol. 81, Issue 5
Mechanism of enhancement of microbial cell hydrophobicity by cationic polymers.
journal, October 1990
- Goldberg, S.; Doyle, R. J.; Rosenberg, M.
- Journal of Bacteriology, Vol. 172, Issue 10
A chemical equilibrium model for metal adsorption onto bacterial surfaces
journal, August 1997
- Fein, Jeremy B.; Daughney, Christopher J.; Yee, Nathan
- Geochimica et Cosmochimica Acta, Vol. 61, Issue 16
Ralstonia metallidurans , a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes
journal, June 2003
- Mergeay, Max; Monchy, Sébastien; Vallaeys, Tatiana
- FEMS Microbiology Reviews, Vol. 27, Issue 2-3
The Biogeochemistry of Gold
journal, October 2009
- Southam, G.; Lengke, M. F.; Fairbrother, L.
- Elements, Vol. 5, Issue 5
Surface transformations of platinum grains from Fifield, New South Wales, Australia
journal, May 2015
- Campbell, S. Gordon; Reith, Frank; Etschmann, Barbara
- American Mineralogist, Vol. 100, Issue 5-6
Precipitation of Silver-Thiosulfate Complex and Immobilization of Silver by Cupriavidus metallidurans CH34
journal, December 2005
- Ledrich, Marie-Laure; Stemmler, Sébastien; Laval-Gilly, Philippe
- BioMetals, Vol. 18, Issue 6
Hybridization peaks in Pt–Cl XANES1Supported in part by the US DOE and by UOP LLC.1
journal, January 2000
- Ankudinov, A. L.; Rehr, J. J.; Bare, Simon R.
- Chemical Physics Letters, Vol. 316, Issue 5-6
Biological role in the transformation of platinum-group mineral grains
journal, March 2016
- Reith, Frank; Zammit, Carla M.; Shar, Sahar S.
- Nature Geoscience, Vol. 9, Issue 4
A review of the PGM industry, deposit models and exploration practices: Implications for Australia's PGM potential
journal, March 2010
- Koek, Monica; Kreuzer, Oliver P.; Maier, Wolfgang D.
- Resources Policy, Vol. 35, Issue 1
Supergene leaching and formation of platinum in alluvium: evidence from Serro, Minas Gerais, Brazil
journal, November 2006
- Cabral, A. R.; Beaudoin, G.; Choquette, M.
- Mineralogy and Petrology, Vol. 90, Issue 1-2
The geochemistry of Witwatersrand-type gold deposits and the possible influence of ancient prokaryotic communities on gold dissolution and precipitation
journal, November 1985
- Mossman, David J.; Dyer, Betsey Dexter
- Precambrian Research, Vol. 30, Issue 4
Morphology of Gold Nanoparticles Synthesized by Filamentous Cyanobacteria from Gold(I)−Thiosulfate and Gold(III)−Chloride Complexes
journal, March 2006
- Lengke, Maggy F.; Fleet, Michael E.; Southam, Gordon
- Langmuir, Vol. 22, Issue 6
Mechanisms of gold biomineralization in the bacterium Cupriavidus metallidurans
journal, October 2009
- Reith, F.; Etschmann, B.; Grosse, C.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 42
Zinc Sorption to Three Gram-Negative Bacteria: Combined Titration, Modeling, and EXAFS Study
journal, March 2006
- Guiné, V.; Spadini, L.; Sarret, G.
- Environmental Science & Technology, Vol. 40, Issue 6
Mononuclear Platinum(II) Complexes Incorporating κ 2 -Carboxylate Ligands: Synthesis, Structure, and Reactivity
journal, July 2009
- Moret, Marc-Etienne; Keller, Sebastian F.; Slootweg, J. Chris
- Inorganic Chemistry, Vol. 48, Issue 14
Aseptic Laboratory Techniques: Plating Methods
journal, January 2012
- Sanders, Erin R.
- Journal of Visualized Experiments, Issue 63
Biosorption and recycling of gold using various microorganisms
journal, January 2004
- Tsuruta, Takehiko
- The Journal of General and Applied Microbiology, Vol. 50, Issue 4
Microbial heavy-metal resistance
journal, June 1999
- Nies, D. H.
- Applied Microbiology and Biotechnology, Vol. 51, Issue 6
Solubility of platinum in aqueous solutions at 25°C and pHs 4 to 10 under oxidizing conditions
journal, December 2001
- Azaroual, Mohamed; Romand, Bruno; Freyssinet, Philippe
- Geochimica et Cosmochimica Acta, Vol. 65, Issue 24
Synthesis of Platinum Nanoparticles by Reaction of Filamentous Cyanobacteria with Platinum(IV)−Chloride Complex
journal, August 2006
- Lengke, Maggy F.; Fleet, Michael E.; Southam, Gordon
- Langmuir, Vol. 22, Issue 17
Influence of geogenic factors on microbial communities in metallogenic Australian soils
journal, June 2012
- Reith, Frank; Brugger, Joel; Zammit, Carla M.
- The ISME Journal, Vol. 6, Issue 11
Effect of cultivation and experimental conditions on the surface reactivity of the metal-resistant bacteria Cupriavidus metallidurans CH34 to protons, cadmium and zinc
journal, January 2007
- Guiné, V.; Martins, J. M. F.; Causse, B.
- Chemical Geology, Vol. 236, Issue 3-4
Iodine in alluvial platinum–palladium nuggets: Evidence for biogenic precious-metal fixation
journal, February 2011
- Cabral, Alexandre R.; Radtke, Martin; Munnik, Frans
- Chemical Geology, Vol. 281, Issue 1-2
Migration of platinum, palladium, and gold in the water systems of platinum deposits
journal, October 2011
- Kubrakova, I. V.; Fortygin, A. V.; Lobov, S. G.
- Geochemistry International, Vol. 49, Issue 11
Chemical controls on the solubility, transport and deposition of platinum and palladium in hydrothermal solutions; a thermodynamic approach
journal, May 1988
- Mountain, Bruce W.; Wood, Scott A.
- Economic Geology, Vol. 83, Issue 3
The geomicrobiology of gold
journal, September 2007
- Reith, Frank; Lengke, Maggy F.; Falconer, Donna
- The ISME Journal, Vol. 1, Issue 7
Gold speciation in natural waters: II. The importance of organic complexing—Experiments with some simple model ligands
journal, June 1990
- Vlassopoulos, Dimitrios; Wood, Scott A.; Mucci, Alfonso
- Geochimica et Cosmochimica Acta, Vol. 54, Issue 6
Applying the Midas touch: Differing toxicity of mobile gold and platinum complexes drives biomineralization in the bacterium Cupriavidus metallidurans
journal, November 2016
- Etschmann, B.; Brugger, J.; Fairbrother, L.
- Chemical Geology, Vol. 438
Platinum-group element (PGE) deposits and occurrences: Mineralization styles, genetic concepts, and exploration criteria
journal, February 2005
- Maier, W. D.
- Journal of African Earth Sciences, Vol. 41, Issue 3
The Geochemical Behavior of Platinum and Palladium in the Weathering Cycle in the Stillwater Complex, Montana
journal, May 1974
- Fuchs, William A.; Rose, Arthur W.
- Economic Geology, Vol. 69, Issue 3
Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals.
journal, January 1985
- Mergeay, M.; Nies, D.; Schlegel, H. G.
- Journal of Bacteriology, Vol. 162, Issue 1
Measurement of live bacteria by Nomarski interference microscopy and stereologic methods as tested with macroscopic rod-shaped models.
journal, January 1988
- Baldwin, W. W.; Bankston, P. W.
- Applied and Environmental Microbiology, Vol. 54, Issue 1
Sites of metal deposition in the cell wall of Bacillus subtilis.
journal, January 1980
- Beveridge, T. J.; Murray, R. G.
- Journal of Bacteriology, Vol. 141, Issue 2
The membrane-induced proton motive force influences the metal binding ability of Bacillus subtilis cell walls.
journal, January 1992
- Urrutia Mera, M.; Kemper, M.; Doyle, R.
- Applied and Environmental Microbiology, Vol. 58, Issue 12
Works referencing / citing this record:
Reflecting on Gold Geomicrobiology Research: Thoughts and Considerations for Future Endeavors
journal, September 2018
- Shuster, Jeremiah; Reith, Frank
- Minerals, Vol. 8, Issue 9
Editorial for Special Issue “Geomicrobiology and Biogeochemistry of Precious Metals”
journal, October 2018
- Reith, Frank; Shuster, Jeremiah
- Minerals, Vol. 8, Issue 10