Immobilisation of Platinum by Cupriavidus metallidurans
Abstract
The metal resistant bacterium Cupriavidus metallidurans CH34, challenged with aqueous platinous and platinic chloride, rapidly immobilized platinum. XANES/EXAFS analysis of these reaction systems demonstrated that platinum binding shifted from chloride to carboxyl functional groups within the bacteria. Pt(IV) was more toxic than Pt(II), presumably due to the oxidative stress imparted by the platinic form. Platinum immobilisation increased with time and with increasing concentrations of platinum. From a bacterial perspective, intracellular platinum concentrations were two to three orders of magnitude greater than the fluid phase, and became saturated at almost molar concentrations in both reaction systems. TEM revealed that C. metallidurans was also able to precipitate nm-scale colloidal platinum, primarily along the cell envelope where energy generation/electron transport occurs. Cells enriched in platinum shed outer membrane vesicles that were enriched in metallic, colloidal platinum, likely representing an important detoxification strategy. Furthermore, the formation of organo-platinum compounds and membrane encapsulated nanophase platinum, supports a role for bacteria in the formation and transport of platinum in natural systems, forming dispersion halos important to metal exploration.
- Authors:
-
- The Univ. of Western Ontario, London, ON (Canada)
- Canadian Light Source, Saskatoon, SK (Canada)
- The Univ. of Adelaide, Adelaide, SA (Australia); CSIRO Land and Water, Glen Osmond, SA (Australia)
- Pacific Northwest Consortium Synchrotron Radiation Facility, Argonne, IL (United States)
- The Univ. of Queensland, St. Lucia (Australia)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- Australian Research Council; Natural Sciences and Engineering Research Council of Canada (NSERC); USDOE
- OSTI Identifier:
- 1464755
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Minerals
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2075-163X
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Cupriavidus metallidurans; biomineralisation; geomicrobiology; platinum; synchrotron
Citation Formats
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., and Southam, Gordon. Immobilisation of Platinum by Cupriavidus metallidurans. United States: N. p., 2018.
Web. doi:10.3390/min8010010.
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., & Southam, Gordon. Immobilisation of Platinum by Cupriavidus metallidurans. United States. doi:10.3390/min8010010.
Campbell, Gordon, MacLean, Lachlan, Reith, Frank, Brewe, Dale, Gordon, Robert A., and Southam, Gordon. Fri .
"Immobilisation of Platinum by Cupriavidus metallidurans". United States. doi:10.3390/min8010010. https://www.osti.gov/servlets/purl/1464755.
@article{osti_1464755,
title = {Immobilisation of Platinum by Cupriavidus metallidurans},
author = {Campbell, Gordon and MacLean, Lachlan and Reith, Frank and Brewe, Dale and Gordon, Robert A. and Southam, Gordon},
abstractNote = {The metal resistant bacterium Cupriavidus metallidurans CH34, challenged with aqueous platinous and platinic chloride, rapidly immobilized platinum. XANES/EXAFS analysis of these reaction systems demonstrated that platinum binding shifted from chloride to carboxyl functional groups within the bacteria. Pt(IV) was more toxic than Pt(II), presumably due to the oxidative stress imparted by the platinic form. Platinum immobilisation increased with time and with increasing concentrations of platinum. From a bacterial perspective, intracellular platinum concentrations were two to three orders of magnitude greater than the fluid phase, and became saturated at almost molar concentrations in both reaction systems. TEM revealed that C. metallidurans was also able to precipitate nm-scale colloidal platinum, primarily along the cell envelope where energy generation/electron transport occurs. Cells enriched in platinum shed outer membrane vesicles that were enriched in metallic, colloidal platinum, likely representing an important detoxification strategy. Furthermore, the formation of organo-platinum compounds and membrane encapsulated nanophase platinum, supports a role for bacteria in the formation and transport of platinum in natural systems, forming dispersion halos important to metal exploration.},
doi = {10.3390/min8010010},
journal = {Minerals},
number = 1,
volume = 8,
place = {United States},
year = {2018},
month = {1}
}
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