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Title: Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls

Cellulose microfibrils are crucial for many of the remarkable mechanical properties of primary cell walls. Nevertheless, many structural features of cellulose microfibril organization in cell walls are not yet fully described. Microscopy techniques provide direct visualization of cell wall organization, and quantification of some aspects of wall microstructure is possible through image processing. Complementary to microscopy techniques, scattering yields structural information in reciprocal space over large sample areas. Using the onion epidermal wall as a model system, we introduce resonant soft X-ray scattering (RSoXS) to directly quantify the average interfibril spacing. Tuning the X-ray energy to the calcium L-edge enhances the contrast between cellulose and pectin due to the localization of calcium ions to homogalacturonan in the pectin matrix. As a consequence, RSoXS profiles reveal an average center-to-center distance between cellulose microfibrils or microfibril bundles of about 20 nm.
Authors:
 [1] ;  [2] ; ORCiD logo [1] ;  [2] ; ORCiD logo [3] ;  [2] ;  [4] ; ORCiD logo [5]
  1. Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemical Engineering
  2. Pennsylvania State Univ., University Park, PA (United States). Dept. of Biology
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source
  4. Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemical Engineering. Dept. of Biomedical Engineering
  5. Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemical Engineering. Dept. of Materials Science and Engineering. Materials Research Inst.
Publication Date:
Grant/Contract Number:
AC02-05CH11231; SC0001090
Type:
Published Article
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Pennsylvania State Univ., University Park, PA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; cell wall; characterization and analytical techniques; soft materials
OSTI Identifier:
1464571
Alternate Identifier(s):
OSTI ID: 1477410

Ye, Dan, Kiemle, Sarah N., Rongpipi, Sintu, Wang, Xuan, Wang, Cheng, Cosgrove, Daniel J., Gomez, Esther W., and Gomez, Enrique D.. Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls. United States: N. p., Web. doi:10.1038/s41598-018-31024-1.
Ye, Dan, Kiemle, Sarah N., Rongpipi, Sintu, Wang, Xuan, Wang, Cheng, Cosgrove, Daniel J., Gomez, Esther W., & Gomez, Enrique D.. Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls. United States. doi:10.1038/s41598-018-31024-1.
Ye, Dan, Kiemle, Sarah N., Rongpipi, Sintu, Wang, Xuan, Wang, Cheng, Cosgrove, Daniel J., Gomez, Esther W., and Gomez, Enrique D.. 2018. "Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls". United States. doi:10.1038/s41598-018-31024-1.
@article{osti_1464571,
title = {Resonant soft X-ray scattering reveals cellulose microfibril spacing in plant primary cell walls},
author = {Ye, Dan and Kiemle, Sarah N. and Rongpipi, Sintu and Wang, Xuan and Wang, Cheng and Cosgrove, Daniel J. and Gomez, Esther W. and Gomez, Enrique D.},
abstractNote = {Cellulose microfibrils are crucial for many of the remarkable mechanical properties of primary cell walls. Nevertheless, many structural features of cellulose microfibril organization in cell walls are not yet fully described. Microscopy techniques provide direct visualization of cell wall organization, and quantification of some aspects of wall microstructure is possible through image processing. Complementary to microscopy techniques, scattering yields structural information in reciprocal space over large sample areas. Using the onion epidermal wall as a model system, we introduce resonant soft X-ray scattering (RSoXS) to directly quantify the average interfibril spacing. Tuning the X-ray energy to the calcium L-edge enhances the contrast between cellulose and pectin due to the localization of calcium ions to homogalacturonan in the pectin matrix. As a consequence, RSoXS profiles reveal an average center-to-center distance between cellulose microfibrils or microfibril bundles of about 20 nm.},
doi = {10.1038/s41598-018-31024-1},
journal = {Scientific Reports},
number = ,
volume = 8,
place = {United States},
year = {2018},
month = {8}
}

Works referenced in this record:

Growth of the plant cell wall
journal, November 2005
  • Cosgrove, Daniel J.
  • Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861
  • DOI: 10.1038/nrm1746

X-Ray Interactions: Photoabsorption, Scattering, Transmission, and Reflection at E = 50-30,000 eV, Z = 1-92
journal, July 1993
  • Henke, B. L.; Gullikson, E. M.; Davis, J. C.
  • Atomic Data and Nuclear Data Tables, Vol. 54, Issue 2, p. 181-342
  • DOI: 10.1006/adnd.1993.1013