skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever

Abstract

Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa and South America had significant adverse impacts on animal health, food security and the pig industry. The disease is generally contained by prevention of exposure through import restrictions (e.g. banning import of live pigs and pork products), localized vaccination programmes and culling of infected or at–risk animals, often at very high cost. Current CSFV–modified live virus vaccines are protective, but do not allow differentiation of infected from vaccinated animals (DIVA), a critical aspect of disease surveillance programmes. Alternatively, first–generation subunit vaccines using the viral protein E2 allow for use of DIVA diagnostic tests, but are slow to induce a protective response, provide limited prevention of vertical transmission and may fail to block viral shedding. CSFV E2 subunit vaccines from a baculovirus/insect cell system have been developed for several vaccination campaigns in Europe and Asia. However, this expression system is considered expensive for a veterinary vaccine and is not ideal for wide–spread deployment. To address the issues of scalability, cost of production and immunogenicity, we have employed an Agrobacterium–mediated transient expression platform in Nicotianamore » benthamiana and formulated the purified antigen in novel oil–in–water emulsion adjuvants. We report the manufacturing of adjuvanted, plant–made CSFV E2 subunit vaccine. The vaccine provided complete protection in challenged pigs, even after single–dose vaccination, which was accompanied by strong virus neutralization antibody responses.« less

Authors:
 [1];  [2];  [3];  [3];  [2];  [2];  [2];  [3];  [4];  [4];  [5];  [6];  [4];  [7];  [7];  [2]; ORCiD logo [3]
  1. Department of Biological and Health Sciences, Texas A&M University Kingsville, Kingsville TX USA
  2. Department of Anatomy and Physiology, Kansas State University, Manhattan KS USA
  3. iBio CDMO, Bryan TX USA
  4. U.S. Department of Homeland Security Science and Technology Directorate, Plum Island Animal Disease Center, Greenport New York USA
  5. Oak Ridge Institute for Science and Education, Plum Island Animal Disease Center Research Participation Program, Oak Ridge TN USA
  6. BioQuest Associates LLC, Plum Island Animal Disease Center, Greenport New York USA
  7. Department of Veterinary Pathobiology, Texas A&M University, College Station TX USA
Publication Date:
Research Org.:
Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1463922
Alternate Identifier(s):
OSTI ID: 1463924; OSTI ID: 1580727
Grant/Contract Number:  
[59-5430-001-23S, NP-103; 2010-ST-061-AG0002; GS-23F-8006H; AC05‐06OR23100]
Resource Type:
Published Article
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
[Journal Name: Plant Biotechnology Journal Journal Volume: 17 Journal Issue: 2]; Journal ID: ISSN 1467-7644
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; veterinary vaccine; classical swine fever; plant‐made pharmaceuticals; DIVA; Nicotiana benthamiana; transient expression

Citation Formats

Laughlin, Richard C., Madera, Rachel, Peres, Yair, Berquist, Brian R., Wang, Lihua, Buist, Sterling, Burakova, Yulia, Palle, Sreenath, Chung, Chungwon J., Rasmussen, Max V., Martel, Erica, Brake, David A., Neilan, John G., Lawhon, Sara D., Adams, L. Garry, Shi, Jishu, and Marcel, Sylvain. Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever. United Kingdom: N. p., 2018. Web. doi:10.1111/pbi.12986.
Laughlin, Richard C., Madera, Rachel, Peres, Yair, Berquist, Brian R., Wang, Lihua, Buist, Sterling, Burakova, Yulia, Palle, Sreenath, Chung, Chungwon J., Rasmussen, Max V., Martel, Erica, Brake, David A., Neilan, John G., Lawhon, Sara D., Adams, L. Garry, Shi, Jishu, & Marcel, Sylvain. Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever. United Kingdom. doi:10.1111/pbi.12986.
Laughlin, Richard C., Madera, Rachel, Peres, Yair, Berquist, Brian R., Wang, Lihua, Buist, Sterling, Burakova, Yulia, Palle, Sreenath, Chung, Chungwon J., Rasmussen, Max V., Martel, Erica, Brake, David A., Neilan, John G., Lawhon, Sara D., Adams, L. Garry, Shi, Jishu, and Marcel, Sylvain. Fri . "Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever". United Kingdom. doi:10.1111/pbi.12986.
@article{osti_1463922,
title = {Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever},
author = {Laughlin, Richard C. and Madera, Rachel and Peres, Yair and Berquist, Brian R. and Wang, Lihua and Buist, Sterling and Burakova, Yulia and Palle, Sreenath and Chung, Chungwon J. and Rasmussen, Max V. and Martel, Erica and Brake, David A. and Neilan, John G. and Lawhon, Sara D. and Adams, L. Garry and Shi, Jishu and Marcel, Sylvain},
abstractNote = {Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa and South America had significant adverse impacts on animal health, food security and the pig industry. The disease is generally contained by prevention of exposure through import restrictions (e.g. banning import of live pigs and pork products), localized vaccination programmes and culling of infected or at–risk animals, often at very high cost. Current CSFV–modified live virus vaccines are protective, but do not allow differentiation of infected from vaccinated animals (DIVA), a critical aspect of disease surveillance programmes. Alternatively, first–generation subunit vaccines using the viral protein E2 allow for use of DIVA diagnostic tests, but are slow to induce a protective response, provide limited prevention of vertical transmission and may fail to block viral shedding. CSFV E2 subunit vaccines from a baculovirus/insect cell system have been developed for several vaccination campaigns in Europe and Asia. However, this expression system is considered expensive for a veterinary vaccine and is not ideal for wide–spread deployment. To address the issues of scalability, cost of production and immunogenicity, we have employed an Agrobacterium–mediated transient expression platform in Nicotiana benthamiana and formulated the purified antigen in novel oil–in–water emulsion adjuvants. We report the manufacturing of adjuvanted, plant–made CSFV E2 subunit vaccine. The vaccine provided complete protection in challenged pigs, even after single–dose vaccination, which was accompanied by strong virus neutralization antibody responses.},
doi = {10.1111/pbi.12986},
journal = {Plant Biotechnology Journal},
number = [2],
volume = [17],
place = {United Kingdom},
year = {2018},
month = {8}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/pbi.12986

Citation Metrics:
Cited by: 3 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Expression of hepatitis B surface antigen in transgenic plants.
journal, December 1992

  • Mason, H. S.; Lam, D. M.; Arntzen, C. J.
  • Proceedings of the National Academy of Sciences, Vol. 89, Issue 24
  • DOI: 10.1073/pnas.89.24.11745

Immunoprotective properties of transgenic plants expressing E2 glycoprotein from CSFV and cysteine protease from Fasciola hepatica
journal, March 2005


Classical swine fever (CSF) marker vaccine
journal, November 2001


Techno-economic analysis of a transient plant-based platform for monoclonal antibody production
journal, August 2016


Genetic diversity of subgenotype 2.1 isolates of classical swine fever virus
journal, July 2016


Plant-based solutions for veterinary immunotherapeutics and prophylactics
journal, December 2014


Development of a classical swine fever subunit marker vaccine and companion diagnostic test
journal, April 2000


Production of antibodies in transgenic plants
journal, November 1989

  • Hiatt, Andrew; Caffferkey, Robert; Bowdish, Katherine
  • Nature, Vol. 342, Issue 6245
  • DOI: 10.1038/342076a0

Efficacy evaluation of the C-strain-based vaccines against the subgenotype 2.1d classical swine fever virus emerging in China
journal, March 2017


Economic aspects of the control of classical swine fever outbreaks in the European Union
journal, April 2000


The 1997–1998 epidemic of classical swine fever in the Netherlands
journal, April 2000


A model to estimate the financial consequences of classical swine fever outbreaks: principles and outcomes
journal, December 1999

  • Meuwissen, Miranda P. M.; Horst, Suzan H.; Huirne, Ruud B. M.
  • Preventive Veterinary Medicine, Vol. 42, Issue 3-4
  • DOI: 10.1016/S0167-5877(99)00079-3

Pigs immunized with a novel E2 subunit vaccine are protected from subgenotype heterologous classical swine fever virus challenge
journal, September 2016


Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever
journal, December 2005


Dissection of a viral autoprotease elucidates a function of a cellular chaperone in proteolysis
journal, January 2006

  • Lackner, T.; Thiel, H. -J.; Tautz, N.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 5
  • DOI: 10.1073/pnas.0508247103

Toward the development of a one-dose classical swine fever subunit vaccine: antigen titration, immunity onset, and duration of immunity
journal, January 2018

  • Madera, Rachel F.; Wang, Lihua; Gong, Wenjie
  • Journal of Veterinary Science, Vol. 19, Issue 3
  • DOI: 10.4142/jvs.2018.19.3.393

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.
journal, May 1999


Edible plants for oral delivery of biopharmaceuticals: Plant-made biopharmaceuticals
journal, May 2016

  • Merlin, Matilde; Pezzotti, Mario; Avesani, Linda
  • British Journal of Clinical Pharmacology, Vol. 83, Issue 1
  • DOI: 10.1111/bcp.12949

Vaccinology of classical swine fever: from lab to field
journal, November 2003


Characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and Mycoplasma hyopneumoniae vaccines
journal, June 2015


Classical swine fever vaccines—State-of-the-art
journal, July 2017


Epidemiology of classical swine fever in Germany in the 1990s
journal, November 2000


Introduction to classical swine fever: virus, disease and control policy
journal, April 2000


Controlling of CSFV in European wild boar using oral vaccination: a review
journal, October 2015


Plant-based oral vaccines against zoonotic and non-zoonotic diseases
journal, August 2016

  • Shahid, Naila; Daniell, Henry
  • Plant Biotechnology Journal, Vol. 14, Issue 11
  • DOI: 10.1111/pbi.12604

Clinical Signs and Epidemiology of Classical Swine Fever: A Review of New Knowledge
journal, January 2003


Immunogenicity of a lettuce-derived vaccine candidate expressing the E2 protein against classical swine fever virus
journal, January 2013

  • Yiu, Jinn-Chin; Liu, Cheng-Wei; Su, Ruei-Yuan
  • Plant Cell, Tissue and Organ Culture (PCTOC), Vol. 113, Issue 3
  • DOI: 10.1007/s11240-013-0290-6

Determination of the onset of the herd-immunity induced by the E2 sub-unit vaccine against classical swine fever virus
journal, February 2000


Commercial-scale biotherapeutics manufacturing facility for plant-made pharmaceuticals
journal, September 2015

  • Holtz, Barry R.; Berquist, Brian R.; Bennett, Lindsay D.
  • Plant Biotechnology Journal, Vol. 13, Issue 8
  • DOI: 10.1111/pbi.12469