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Title: Tandem column isolation of zirconium-89 from cyclotron bombarded yttrium targets using an automated fluidic platform: Anion exchange to hydroxamate resin columns

Journal Article · · Journal of Chromatography
ORCiD logo [1];  [1];  [1];  [1];  [2]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Nuclear Sciences Division
  2. Oregon Health & Science Univ., Portland, OR (United States). Center for Radiochemistry Research, Department of Diagnostic Radiology

The development of a tandem column purification method for the preparation of high-purity 89Zr(IV) oxalate is presented. The primary column was a macroporous strongly basic anion exchange resin on styrene divinylbenzene co-polymer. The secondary column, with an internal volume of 33 μL, was packed with hydroxamate resin. A condition of inverted selectivity was developed, whereby the 89Zr eluent solution for the primary column is equivalent to the 89Zr load solution for the secondary column. The ability to transfer 89Zr from one column to the next allows two sequential column clean-up methods to be performed prior to the final elution of the 89Zr(IV) oxalate. This approach assures delivery of high purity 89Zr product and assures a 89Zr product that is eluted in a substantially smaller volume than is possible when using the traditionally-employed single hydroxamate resin column method. The tandem column purification process has been implemented into a prototype automated fluidic system. The system is configured with on-line gamma detection so column effluents can be monitored in near-real time. The automated method was tested using seven cyclotron bombarded Y foil targets. It was found that 95.1 ± 1.3% of the 89Zr present in the foils was recovered in the secondary column elution fraction. Furthermore, elution peak analysis of several 89Zr elution profile radiochromatograms made possible the determination of 89Zr recovery as a function of volume; a 89Zr product volume that contains 90% of the mean secondary column elution peak can be obtained in 0.29 ± 0.06 mL (representing 86 ± 5% of the 89Zr activity in the target). This product volume represents a significant improvement in radionuclide product concentration over the predominant method used in the field. In addition to the reduced 89Zr product elution volume, titrations of the 89Zr product with deferoxamine mesylate salt across two preparatory methods resulted in mean effective specific activity (ESA) values of 279 and 340 T Bq·mmole-1 and mean bindable metals concentrations ([MB]) of 13.5 and 16.7 nmole·g-1. In conclusion, these ESA and [MB] values infer that the 89Zr(IV) oxalate product resulting from this tandem column isolation method has the highest purity reported to date.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
S10-RR017229; AC0576RL01830; AC06-67RLO 1830
OSTI ID:
1463331
Alternate ID(s):
OSTI ID: 1702371
Report Number(s):
PNNL-SA-134266; PII: S002196731830791X
Journal Information:
Journal of Chromatography, Vol. 1567, Issue C; ISSN 0021-9673
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

References (17)

Zirconium-89 Labeled Antibodies: A New Tool for Molecular Imaging in Cancer Patients journal January 2014
PET Tracers Based on Zirconium-89 journal April 2011
PET imaging with 89Zr: From radiochemistry to the clinic journal January 2013
89Zr Radiochemistry for Positron Emission Tomography journal September 2011
Production of highly pure no-carrier added 89Zr for the labelling of antibodies with a positron emitter journal December 1994
Standardized methods for the production of high specific-activity zirconium-89 journal October 2009
Semi-automated production of 89 Zr-oxalate/ 89 Zr-chloride and the potential of 89 Zr-chloride in radiopharmaceutical compounding journal January 2016
Routine Production of 89Zr Using an Automated Module journal July 2013
Hydroxamate column-based purification of zirconium-89 (89Zr) using an automated fluidic platform journal February 2018
Specific activity measurement of 64Cu: A comparison of methods journal August 2014
Open letter to journal editors on: International Consensus Radiochemistry Nomenclature Guidelines journal February 2018
Purification of radionuclides for nuclear medicine: The multicolumn selectivity inversion generator concept journal January 2003
Automated two column generator systems for medical radionuclides journal November 2009
Optimized anion exchange column isolation of zirconium-89 (89Zr) from yttrium cyclotron target: Method development and implementation on an automated fluidic platform journal April 2018
Recent Advances in Zirconium-89 Chelator Development journal March 2018
A simple thick target for production of 89Zr using an 11 MeV cyclotron journal April 2017
Evaluation of desferal as a bifunctional chelating agent for labeling antibodies with Zr-89
  • Meijs, Wilma E.; Herscheid, Jacobus D. M.; Haisma, Hidde J.
  • International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes, Vol. 43, Issue 12 https://doi.org/10.1016/0883-2889(92)90170-J
journal December 1992

Cited By (1)

Development of an autonomous solvent extraction system to isolate astatine-211 from dissolved cyclotron bombarded bismuth targets journal December 2019