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Title: Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions

Abstract

Free fatty acids (FFA) are an attractive platform chemical that serves as a functional intermediate in metabolic pathways for producing oleochemicals. Many groups have established strains of Escherichia coli capable of producing various chain-length mixtures of FFA by heterologous expression of acyl-ACP thioesterases. For example, high levels of dodecanoic acid are produced by an E. coli strain expressing the Umbellularia californica FatB2 thioesterase, BTE. Prior studies achieved high dodecanoic acid yields and productivities under phosphate-limiting media conditions. In an effort to understand the metabolic and physiological changes that led to increased FFA production, the tanscriptome of this strain was assessed as a function of nutrient limitation and growth rate. FFA generation under phosphate limitation led to consistent changes in transporter expression, osmoregulation, and central metabolism. Guided by these results, targeted knockouts led to a further ~11% in yield in FFA.

Authors:
 [1];  [1]; ORCiD logo [1]
  1. Univ. of Wisconsin, Madison, WI (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1463132
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Industrial Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 44; Journal Issue: 4-5; Journal ID: ISSN 1367-5435
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Youngquist, J. Tyler, Korosh, Travis C., and Pfleger, Brian F.. Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions. United States: N. p., 2016. Web. doi:10.1007/s10295-016-1846-3.
Youngquist, J. Tyler, Korosh, Travis C., & Pfleger, Brian F.. Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions. United States. doi:10.1007/s10295-016-1846-3.
Youngquist, J. Tyler, Korosh, Travis C., and Pfleger, Brian F.. Thu . "Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions". United States. doi:10.1007/s10295-016-1846-3. https://www.osti.gov/servlets/purl/1463132.
@article{osti_1463132,
title = {Functional genomics analysis of free fatty acid production under continuous phosphate limiting conditions},
author = {Youngquist, J. Tyler and Korosh, Travis C. and Pfleger, Brian F.},
abstractNote = {Free fatty acids (FFA) are an attractive platform chemical that serves as a functional intermediate in metabolic pathways for producing oleochemicals. Many groups have established strains of Escherichia coli capable of producing various chain-length mixtures of FFA by heterologous expression of acyl-ACP thioesterases. For example, high levels of dodecanoic acid are produced by an E. coli strain expressing the Umbellularia californica FatB2 thioesterase, BTE. Prior studies achieved high dodecanoic acid yields and productivities under phosphate-limiting media conditions. In an effort to understand the metabolic and physiological changes that led to increased FFA production, the tanscriptome of this strain was assessed as a function of nutrient limitation and growth rate. FFA generation under phosphate limitation led to consistent changes in transporter expression, osmoregulation, and central metabolism. Guided by these results, targeted knockouts led to a further ~11% in yield in FFA.},
doi = {10.1007/s10295-016-1846-3},
journal = {Journal of Industrial Microbiology and Biotechnology},
number = 4-5,
volume = 44,
place = {United States},
year = {2016},
month = {10}
}

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Works referenced in this record:

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants the Keio collection
journal, February 2006

  • Baba, Tomoya; Ara, Takeshi; Hasegawa, Miki
  • Molecular Systems Biology, Vol. 2, Article No. 2006.0008
  • DOI: 10.1038/msb4100050

Growth Phase-Dependent Expression of Drug Exporters in Escherichia coli and Its Contribution to Drug Tolerance
journal, August 2006

  • Kobayashi, A.; Hirakawa, H.; Hirata, T.
  • Journal of Bacteriology, Vol. 188, Issue 16, p. 5693-5703
  • DOI: 10.1128/JB.00217-06

A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes
journal, June 2010

  • Lennen, Rebecca M.; Braden, Drew J.; West, Ryan M.
  • Biotechnology and Bioengineering, Vol. 106, Issue 2, p. 193-202
  • DOI: 10.1002/bit.22660

Kinetic modeling of free fatty acid production in Escherichia coli based on continuous cultivation of a plasmid free strain
journal, January 2012

  • Youngquist, J. Tyler; Lennen, Rebecca M.; Ranatunga, Don R.
  • Biotechnology and Bioengineering, Vol. 109, Issue 6, p. 1518-1527
  • DOI: 10.1002/bit.24420