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Title: Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae

Journal Article · · Biotechnology and Bioengineering
DOI: https://doi.org/10.1002/bit.26799 · OSTI ID:1463099
 [1];  [2];  [2];  [3];  [4]; ORCiD logo [2]
  1. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  3. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Northwestern Univ. Feinberg School of Medicine, Chicago, IL (United States)
  4. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Univ. of Colorado, Boulder, CO (United States)

Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio–degradable surfactant, ethyl–β–d–glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT–1) and intracellular β–glucosidase (GH1–1) originating from Neurospora crassa. We identified the formation of ethyl–β–d–glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1–1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl–β–d–glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g∙L-1∙h-1, respectively, during the SSF of Avicel PH–101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co–production of a value–added chemical (alkyl–glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1–1 in engineered S. cerevisiae. In conclusion, this co–production could have a substantial effect on overall techno–economic feasibility of SSF of cellulose.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1463099
Report Number(s):
NREL/JA-5100-72076
Journal Information:
Biotechnology and Bioengineering, Vol. 115, Issue 12; ISSN 0006-3592
Publisher:
WileyCopyright Statement
Country of Publication:
United States
Language:
English

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