Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae

Jayakody, Lahiru Niroshan; Liu, Jing -Jing; Yun, Eun Ju; Turner, Timothy Lee; Oh, Eun Joong; Jin, Yong -Su

doi:10.1002/bit.26799

Title: Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae

Journal Article · 16 July 2018 · Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.26799 · OSTI ID:1463099

Jayakody, Lahiru Niroshan ^[1]; Liu, Jing -Jing ^[2]; Yun, Eun Ju ^[2]; Turner, Timothy Lee ^[3]; Oh, Eun Joong ^[4];

^[2]

Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Northwestern Univ. Feinberg School of Medicine, Chicago, IL (United States)
Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Univ. of Colorado, Boulder, CO (United States)

Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio–degradable surfactant, ethyl–β–d–glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT–1) and intracellular β–glucosidase (GH1–1) originating from Neurospora crassa. We identified the formation of ethyl–β–d–glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1–1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl–β–d–glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g∙L^-1∙h^-1, respectively, during the SSF of Avicel PH–101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co–production of a value–added chemical (alkyl–glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1–1 in engineered S. cerevisiae. In conclusion, this co–production could have a substantial effect on overall techno–economic feasibility of SSF of cellulose.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1463099

Report Number(s):: NREL/JA-5100-72076

Journal Information:: Biotechnology and Bioengineering, Vol. 115, Issue 12; ISSN 0006-3592

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

References (57)

Fungal and other β-d-glucosidases — Their properties and applications Woodward, Jonathan; Wiseman, Alan Enzyme and Microbial Technology, Vol. 4, Issue 2 https://doi.org/10.1016/0141-0229(82)90084-9	journal	March 1982
Role of water activity in ethanol fermentations Jones, Rodney P.; Greenfield, Paul F. Biotechnology and Bioengineering, Vol. 28, Issue 1 https://doi.org/10.1002/bit.260280106	journal	January 1986
Rational and Evolutionary Engineering Approaches Uncover a Small Set of Genetic Changes Efficient for Rapid Xylose Fermentation in Saccharomyces cerevisiae Kim, Soo Rin; Skerker, Jeffrey M.; Kang, Wei PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0057048	journal	February 2013
Cost evaluation of cellulase enzyme for industrial-scale cellulosic ethanol production based on rigorous Aspen Plus modeling Liu, Gang; Zhang, Jian; Bao, Jie Bioprocess and Biosystems Engineering, Vol. 39, Issue 1 https://doi.org/10.1007/s00449-015-1497-1	journal	November 2015
Factors affecting the specificity of β-glucosidase from Fusarium oxysporum in enzymatic synthesis of alkyl-β-d-glucosides Makropoulou, M.; Christakopoulos, P.; Tsitsimpikou, C. International Journal of Biological Macromolecules, Vol. 22, Issue 2 https://doi.org/10.1016/S0141-8130(97)00092-5	journal	April 1998
A simple method to determine the water activity of ethanol-containing samples Hallsworth, J. E.; Nomura, Y. Biotechnology and Bioengineering, Vol. 62, Issue 2 https://doi.org/10.1002/(SICI)1097-0290(19990120)62:2<242::AID-BIT15>3.0.CO;2-R	journal	January 1999
Simultaneous Utilization of Cellobiose, Xylose, and Acetic Acid from Lignocellulosic Biomass for Biofuel Production by an Engineered Yeast Platform Wei, Na; Oh, Eun Joong; Million, Gyver ACS Synthetic Biology, Vol. 4, Issue 6 https://doi.org/10.1021/sb500364q	journal	November 2014
Lactose fermentation by engineered Saccharomyces cerevisiae capable of fermenting cellobiose Liu, Jing-Jing; Zhang, Guo-Chang; Oh, Eun Joong Journal of Biotechnology, Vol. 234 https://doi.org/10.1016/j.jbiotec.2016.07.018	journal	September 2016
Simultaneous co-fermentation of mixed sugars: a promising strategy for producing cellulosic ethanol Kim, Soo Rin; Ha, Suk-Jin; Wei, Na Trends in Biotechnology, Vol. 30, Issue 5 https://doi.org/10.1016/j.tibtech.2012.01.005	journal	May 2012
Synergistic Saccharification, and Direct Fermentation to Ethanol, of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplaying Three Types of Cellulolytic Enzyme Fujita, Y.; Ito, J.; Ueda, M. Applied and Environmental Microbiology, Vol. 70, Issue 2, p. 1207-1212 https://doi.org/10.1128/AEM.70.2.1207-1212.2004	journal	February 2004
The use of octyl β-d-glucoside as detergent for hog kidney brush border membrane Lin, J. T.; Riedel, S.; Kinne, R. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 557, Issue 1 https://doi.org/10.1016/0005-2736(79)90100-7	journal	October 1979
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds Mumberg, Dominik; Müller, Rolf; Funk, Martin Gene, Vol. 156, Issue 1 https://doi.org/10.1016/0378-1119(95)00037-7	journal	April 1995
Transglycosylation: A Key Reaction to Access Alkylpolyglycosides from Lignocellulosic Biomass Jérôme, François; Marinkovic, Sinisa; Estrine, Boris ChemSusChem, Vol. 11, Issue 9 https://doi.org/10.1002/cssc.201800265	journal	April 2018
The Techno-Economic Basis for Coproduct Manufacturing To Enable Hydrocarbon Fuel Production from Lignocellulosic Biomass Biddy, Mary J.; Davis, Ryan; Humbird, David ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 6 https://doi.org/10.1021/acssuschemeng.6b00243	journal	May 2016
Mass spectrometry in the structural analysis of flavonoids Cuyckens, Filip; Claeys, Magda Journal of Mass Spectrometry, Vol. 39, Issue 1 https://doi.org/10.1002/jms.585	journal	January 2004
Gene Amplification on Demand Accelerates Cellobiose Utilization in Engineered Saccharomyces cerevisiae Oh, Eun Joong; Skerker, Jeffrey M.; Kim, Soo Rin Applied and Environmental Microbiology, Vol. 82, Issue 12 https://doi.org/10.1128/AEM.00410-16	journal	April 2016
Cellulose: Fascinating Biopolymer and Sustainable Raw Material Klemm, Dieter; Heublein, Brigitte; Fink, Hans-Peter Angewandte Chemie International Edition, Vol. 44, Issue 22 https://doi.org/10.1002/anie.200460587	journal	May 2005
Effect of Reduction in Yeast and Enzyme Concentrations in a Simultaneous- Saccharification-and-Fermentation–Based Bioethanol Process: Technical and Economic Evaluation Wingren, Anders; Galbe, Mats; Roslander, Christian Applied Biochemistry and Biotechnology, Vol. 122, Issue 1-3 https://doi.org/10.1385/ABAB:122:1-3:0485	journal	January 2005
A review of cellulosic biofuel commercial-scale projects in the United States Brown, Tristan R.; Brown, Robert C. Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 3 https://doi.org/10.1002/bbb.1387	journal	February 2013
Cellulose conversion into alkylglycosides in the ionic liquid 1-butyl-3-methylimidazolium chloride Ignatyev, Igor A.; Mertens, Pascal G. N.; Van Doorslaer, Charlie Green Chemistry, Vol. 12, Issue 10 https://doi.org/10.1039/c0gc00192a	journal	January 2010
β-Glucosidase catalyzed synthesis of octyl-β-d-glucopyranoside using whole cells of Pichia etchellsii in micro aqueous media Rather, Mohd. Younis; Mishra, Saroj; Chand, Subhash Journal of Biotechnology, Vol. 150, Issue 4 https://doi.org/10.1016/j.jbiotec.2010.09.933	journal	December 2010
Glycosidases: a key to tailored carbohydrates Bojarová, Pavla; Křen, Vladimír Trends in Biotechnology, Vol. 27, Issue 4 https://doi.org/10.1016/j.tibtech.2008.12.003	journal	April 2009
Comparison of Conventional and Lignin-Rich Microcrystalline Cellulose Vanhatalo, Kari Mikael; Maximova, Natalia; Perander, Anna-Maija BioResources, Vol. 11, Issue 2 https://doi.org/10.15376/biores.11.2.4037-4054	journal	February 2016
Purification and Characterization of an Extracellular β-Glucosidase II with High Hydrolysis and Transglucosylation Activities from Aspergillus niger Yan, Tsong-Rong; Lin, Yi-Hui; Lin, Chun-Leih Journal of Agricultural and Food Chemistry, Vol. 46, Issue 2 https://doi.org/10.1021/jf9702499	journal	February 1998
Derivation and synthesis of renewable surfactants Foley, Patrick; Kermanshahi pour, Azadeh; Beach, Evan S. Chem. Soc. Rev., Vol. 41, Issue 4 https://doi.org/10.1039/C1CS15217C	journal	January 2012
Biotransformation of methyl-β-d-glucopyranoside to higher chain alkyl glucosides by cell bound β-glucosidase of Pichia etchellsii Younis Rather, Mohd.; Mishra, Saroj; Verma, Vijeshwar Bioresource Technology, Vol. 107 https://doi.org/10.1016/j.biortech.2011.11.061	journal	March 2012
Introduction of a Series of Alkyl Thiomaltosides, Useful New Non-Ionic Detergents, to Membrane Biochemistry1 Izawa, Satoko; Sakai-Tomita, Yuki; Kinomura, Keisuke The Journal of Biochemistry, Vol. 113, Issue 5 https://doi.org/10.1093/oxfordjournals.jbchem.a124085	journal	May 1993
Production of alkyl glucoside from cellooligosaccharides using yeast strains displaying Aspergillus aculeatus β-glucosidase 1 Ito, Junji; Ebe, Takumi; Shibasaki, Seiji Journal of Molecular Catalysis B: Enzymatic, Vol. 49, Issue 1-4, p. 92-97 https://doi.org/10.1016/j.molcatb.2007.08.008	journal	November 2007
Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion Zhang, Guo-Chang; Liu, Jing-Jing; Kong, In Iok Current Opinion in Chemical Biology, Vol. 29 https://doi.org/10.1016/j.cbpa.2015.09.008	journal	December 2015
Features of promising technologies for pretreatment of lignocellulosic biomass Mosier, Nathan; Wyman, Charles; Dale, Bruce Bioresource Technology, Vol. 96, Issue 6, p. 673-686 https://doi.org/10.1016/j.biortech.2004.06.025	journal	April 2005
Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process Philippidis, George P.; Smith, Tammy K.; Wyman, Charles E. Biotechnology and Bioengineering, Vol. 41, Issue 9, p. 846-853 https://doi.org/10.1002/bit.260410903	journal	April 1993
Bio-ethanol – the fuel of tomorrow from the residues of today Hahn-Hägerdal, B.; Galbe, M.; Gorwa-Grauslund, M. F. Trends in Biotechnology, Vol. 24, Issue 12, p. 549-556 https://doi.org/10.1016/j.tibtech.2006.10.004	journal	December 2006
Microbial Cellulases and Their Industrial Applications Kuhad, Ramesh Chander; Gupta, Rishi; Singh, Ajay Enzyme Research, Vol. 2011 https://doi.org/10.4061/2011/280696	journal	January 2011
Glycosidase-catalysed synthesis of alkyl glycosides van Rantwijk, F.; Woudenberg-van Oosterom, M.; Sheldon, R. A. Journal of Molecular Catalysis B: Enzymatic, Vol. 6, Issue 6 https://doi.org/10.1016/S1381-1177(99)00042-9	journal	June 1999
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass Steen, Eric J.; Kang, Yisheng; Bokinsky, Gregory Nature, Vol. 463, Issue 7280, p. 559-562 https://doi.org/10.1038/nature08721	journal	January 2010
Ethyl glucoside in human urine following dietary exposure: detection by 1H NMR spectroscopy as a result of metabonomic screening of humans Teague, Claire; Holmes, Elaine; Maibaum, Elaine The Analyst, Vol. 129, Issue 3 https://doi.org/10.1039/b314316n	journal	January 2004
Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels Balan, Venkatesh; Chiaramonti, David; Kumar, Sandeep Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 6 https://doi.org/10.1002/bbb.1436	journal	August 2013
Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates Meng, Xianzhi; Ragauskas, Arthur Jonas Current Opinion in Biotechnology, Vol. 27 https://doi.org/10.1016/j.copbio.2014.01.014	journal	June 2014
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation Ha, S. -J.; Galazka, J. M.; Rin Kim, S. Proceedings of the National Academy of Sciences, Vol. 108, Issue 2 https://doi.org/10.1073/pnas.1010456108	journal	December 2010
Structural Determination of Glucosylceramides in the Distillation Remnants of Shochu, the Japanese Traditional Liquor, and Its Production by Aspergillus kawachii Hirata, Miyo; Tsuge, Keisuke; Jayakody, Lahiru N. Journal of Agricultural and Food Chemistry, Vol. 60, Issue 46 https://doi.org/10.1021/jf303117e	journal	November 2012
Direct Conversion of Cellulose into Alkyl Glycoside Surfactants Puga, Alberto V.; Corma, Avelino ChemistrySelect, Vol. 2, Issue 8 https://doi.org/10.1002/slct.201700389	journal	March 2017
Preparation of Long-Chain Alkyl Glucoside Surfactants by One-Step Direct Fischer Glucosidation, and by Transacetalation of Butyl Glucosides, on Beta Zeolite Catalysts Corma, A.; Iborra, S.; Miquel, S. Journal of Catalysis, Vol. 180, Issue 2 https://doi.org/10.1006/jcat.1998.2272	journal	December 1998
Cellulosic Biofuels--Got Gasoline? Regalbuto, J. R. Science, Vol. 325, Issue 5942 https://doi.org/10.1126/science.1174581	journal	August 2009
Outlook for cellulase improvement: Screening and selection strategies Percival Zhang, Y.-H.; Himmel, Michael E.; Mielenz, Jonathan R. Biotechnology Advances, Vol. 24, Issue 5, p. 452-481 https://doi.org/10.1016/j.biotechadv.2006.03.003	journal	September 2006
Glucosylceramide Contained in Koji Mold-Cultured Cereal Confers Membrane and Flavor Modification and Stress Tolerance to Saccharomyces cerevisiae during Coculture Fermentation Sawada, Kazutaka; Sato, Tomoya; Hamajima, Hiroshi Applied and Environmental Microbiology, Vol. 81, Issue 11 https://doi.org/10.1128/AEM.00454-15	journal	March 2015
Techno-Economic Evaluation of Producing Ethanol from Softwood: Comparison of SSF and SHF and Identification of Bottlenecks Wingren, Anders; Galbe, Mats; Zacchi, Guido Biotechnology Progress, Vol. 19, Issue 4 https://doi.org/10.1021/bp0340180	journal	January 2003
Optimization of enzyme complexes for lignocellulose hydrolysis Berlin, Alex; Maximenko, Vera; Gilkes, Neil Biotechnology and Bioengineering, Vol. 97, Issue 2, p. 287-296 https://doi.org/10.1002/bit.21238	journal	January 2007
Improved ethanol production by engineered Saccharomyces cerevisiae expressing a mutated cellobiose transporter during simultaneous saccharification and fermentation Lee, Won-Heong; Jin, Yong-Su Journal of Biotechnology, Vol. 245 https://doi.org/10.1016/j.jbiotec.2017.01.018	journal	March 2017
Sugar-based surfactants for consumer products and technical applications Hill, Karlheinz; Rhode, Oliver Lipid - Fett, Vol. 101, Issue 1 https://doi.org/10.1002/(SICI)1521-4133(19991)101:1<25::AID-LIPI25>3.0.CO;2-N	journal	January 1999
Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production Singhania, Reeta Rani; Patel, Anil Kumar; Sukumaran, Rajeev K. Bioresource Technology, Vol. 127 https://doi.org/10.1016/j.biortech.2012.09.012	journal	January 2013
Production of first and second generation biofuels: A comprehensive review Naik, S. N.; Goud, Vaibhav V.; Rout, Prasant K. Renewable and Sustainable Energy Reviews, Vol. 14, Issue 2 https://doi.org/10.1016/j.rser.2009.10.003	journal	February 2010
Efficient Production and Separation of Biodegradable Surfactants from Cellulose in 1-Butyl-3-Methylimidazolium Chloride Puga, Alberto V.; Corma, Avelino ChemSusChem, Vol. 7, Issue 12 https://doi.org/10.1002/cssc.201402722	journal	October 2014
Transformation of Cellulose into Biodegradable Alkyl Glycosides by Following Two Different Chemical Routes Villandier, Nicolas; Corma, Avelino ChemSusChem, Vol. 4, Issue 4 https://doi.org/10.1002/cssc.201000371	journal	March 2011
Foreign gene expression in yeast: a review Romanos, Michael A.; Scorer, Carol A.; Clare, Jeffrey J. Yeast, Vol. 8, Issue 6 https://doi.org/10.1002/yea.320080602	journal	June 1992
Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels Balan, Venkatesh; Chiaramonti, David; Kumar, Sandeep Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 6 https://doi.org/10.1002/bbb.1452	journal	November 2013
Mass spectrometry in the structural analysis of flavonoids Cuyckens, Filip; Claeys, Magda Journal of Mass Spectrometry, Vol. 39, Issue 4 https://doi.org/10.1002/jms.622	journal	April 2004
Cellulose: Fascinating Biopolymer and Sustainable Raw Material Klemm, Dieter; Heublein, Brigitte; Fink, Hans-Peter ChemInform, Vol. 36, Issue 36 https://doi.org/10.1002/chin.200536238	journal	September 2005

Cited By (2)

Deficiency of β-Glucosidase Beneficial for the Simultaneous Saccharification and Lipid Production by the Oleaginous Yeast Lipomyces starkeyi Gou, Qingling; Tang, Mou; Wang, Yanan Applied Biochemistry and Biotechnology, Vol. 190, Issue 2 https://doi.org/10.1007/s12010-019-03129-4	journal	September 2019
Selective radical depolymerization of cellulose to glucose induced by high frequency ultrasound Haouache, Somia; Karam, Ayman; Chave, Tony Chemical Science, Vol. 11, Issue 10 https://doi.org/10.1039/d0sc00020e	journal	January 2020

Similar Records

Development of a novel cellulase biosensor that detects crystalline cellulose hydrolysis using a transcriptional regulator

Journal Article · 2018 · Biochemical and Biophysical Research Communications · OSTI ID:23127519

Kwon, Kil Koang; Yeom, Soo-Jin; Lee, Dae-Hee; +2 more

Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered improves ethanol production
Journal Article · 2018 · Biotechnology for Biofuels · OSTI ID:1618728

Hon, Shuen; Holwerda, Evert K.; Worthen, Robert S.; +6 more

Unveiling a classical mutant in the context of the GH₃ β-glucosidase family in Neurospora crassa
Journal Article · 2024 · AMB Express · OSTI ID:2282268

Zhang, Yuxin; Nada, Basant; Baker, Scott E.; +4 more

Related Subjects

09 BIOMASS FUELS
cellulose
simultaneous saccharification and fermentation
transglycosylation
yeast

Title: Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae

Citation Formats

References (57)

Cited By (2)

Similar Records

Related Subjects