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Title: Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae

Abstract

Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio–degradable surfactant, ethyl–β–d–glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT–1) and intracellular β–glucosidase (GH1–1) originating from Neurospora crassa. We identified the formation of ethyl–β–d–glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1–1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl–β–d–glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g∙L -1∙h -1, respectively, during the SSF of Avicel PH–101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co–production of a value–added chemical (alkyl–glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1–1 in engineered S. cerevisiae. In conclusion, this co–production could have a substantial effect on overall techno–economic feasibility of SSF of cellulose.

Authors:
 [1];  [2];  [2];  [3];  [4]; ORCiD logo [2]
  1. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
  2. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  3. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Northwestern Univ. Feinberg School of Medicine, Chicago, IL (United States)
  4. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Univ. of Colorado, Boulder, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1463099
Report Number(s):
NREL/JA-5100-72076
Journal ID: ISSN 0006-3592
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Volume: 115; Journal Issue: 12; Journal ID: ISSN 0006-3592
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; cellulose; simultaneous saccharification and fermentation; transglycosylation; yeast

Citation Formats

Jayakody, Lahiru Niroshan, Liu, Jing -Jing, Yun, Eun Ju, Turner, Timothy Lee, Oh, Eun Joong, and Jin, Yong -Su. Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae. United States: N. p., 2018. Web. doi:10.1002/bit.26799.
Jayakody, Lahiru Niroshan, Liu, Jing -Jing, Yun, Eun Ju, Turner, Timothy Lee, Oh, Eun Joong, & Jin, Yong -Su. Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae. United States. doi:10.1002/bit.26799.
Jayakody, Lahiru Niroshan, Liu, Jing -Jing, Yun, Eun Ju, Turner, Timothy Lee, Oh, Eun Joong, and Jin, Yong -Su. Mon . "Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae". United States. doi:10.1002/bit.26799. https://www.osti.gov/servlets/purl/1463099.
@article{osti_1463099,
title = {Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae},
author = {Jayakody, Lahiru Niroshan and Liu, Jing -Jing and Yun, Eun Ju and Turner, Timothy Lee and Oh, Eun Joong and Jin, Yong -Su},
abstractNote = {Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio–degradable surfactant, ethyl–β–d–glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT–1) and intracellular β–glucosidase (GH1–1) originating from Neurospora crassa. We identified the formation of ethyl–β–d–glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1–1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl–β–d–glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g∙L-1∙h-1, respectively, during the SSF of Avicel PH–101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co–production of a value–added chemical (alkyl–glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1–1 in engineered S. cerevisiae. In conclusion, this co–production could have a substantial effect on overall techno–economic feasibility of SSF of cellulose.},
doi = {10.1002/bit.26799},
journal = {Biotechnology and Bioengineering},
number = 12,
volume = 115,
place = {United States},
year = {2018},
month = {7}
}

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