Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae
Abstract
Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio–degradable surfactant, ethyl–β–d–glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT–1) and intracellular β–glucosidase (GH1–1) originating from Neurospora crassa. We identified the formation of ethyl–β–d–glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1–1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl–β–d–glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g∙L-1∙h-1, respectively, during the SSF of Avicel PH–101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co–production of a value–added chemical (alkyl–glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1–1 in engineered S. cerevisiae. In conclusion, this co–production could have a substantial effect on overall techno–economic feasibility of SSF of cellulose.
- Authors:
-
- Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
- Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Northwestern Univ. Feinberg School of Medicine, Chicago, IL (United States)
- Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Univ. of Colorado, Boulder, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1463099
- Report Number(s):
- NREL/JA-5100-72076
Journal ID: ISSN 0006-3592
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology and Bioengineering
- Additional Journal Information:
- Journal Volume: 115; Journal Issue: 12; Journal ID: ISSN 0006-3592
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; cellulose; simultaneous saccharification and fermentation; transglycosylation; yeast
Citation Formats
Jayakody, Lahiru Niroshan, Liu, Jing -Jing, Yun, Eun Ju, Turner, Timothy Lee, Oh, Eun Joong, and Jin, Yong -Su. Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae. United States: N. p., 2018.
Web. doi:10.1002/bit.26799.
Jayakody, Lahiru Niroshan, Liu, Jing -Jing, Yun, Eun Ju, Turner, Timothy Lee, Oh, Eun Joong, & Jin, Yong -Su. Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae. United States. https://doi.org/10.1002/bit.26799
Jayakody, Lahiru Niroshan, Liu, Jing -Jing, Yun, Eun Ju, Turner, Timothy Lee, Oh, Eun Joong, and Jin, Yong -Su. Mon .
"Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae". United States. https://doi.org/10.1002/bit.26799. https://www.osti.gov/servlets/purl/1463099.
@article{osti_1463099,
title = {Direct conversion of cellulose into ethanol and ethyl-β-D-glucoside via engineered Saccharomyces cerevisiae},
author = {Jayakody, Lahiru Niroshan and Liu, Jing -Jing and Yun, Eun Ju and Turner, Timothy Lee and Oh, Eun Joong and Jin, Yong -Su},
abstractNote = {Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a bio–degradable surfactant, ethyl–β–d–glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT–1) and intracellular β–glucosidase (GH1–1) originating from Neurospora crassa. We identified the formation of ethyl–β–d–glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1–1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl–β–d–glucoside/g cellulose at a rate of 0.30 ± 0.02 g/L/h and 0.09 ± 01 g∙L-1∙h-1, respectively, during the SSF of Avicel PH–101 cellulose, supplemented only with Celluclast 1.5L. Herein, we report a possible co–production of a value–added chemical (alkyl–glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1–1 in engineered S. cerevisiae. In conclusion, this co–production could have a substantial effect on overall techno–economic feasibility of SSF of cellulose.},
doi = {10.1002/bit.26799},
journal = {Biotechnology and Bioengineering},
number = 12,
volume = 115,
place = {United States},
year = {2018},
month = {7}
}
Works referenced in this record:
Fungal and other β-d-glucosidases — Their properties and applications
journal, March 1982
- Woodward, Jonathan; Wiseman, Alan
- Enzyme and Microbial Technology, Vol. 4, Issue 2
Role of water activity in ethanol fermentations
journal, January 1986
- Jones, Rodney P.; Greenfield, Paul F.
- Biotechnology and Bioengineering, Vol. 28, Issue 1
Rational and Evolutionary Engineering Approaches Uncover a Small Set of Genetic Changes Efficient for Rapid Xylose Fermentation in Saccharomyces cerevisiae
journal, February 2013
- Kim, Soo Rin; Skerker, Jeffrey M.; Kang, Wei
- PLoS ONE, Vol. 8, Issue 2
Cost evaluation of cellulase enzyme for industrial-scale cellulosic ethanol production based on rigorous Aspen Plus modeling
journal, November 2015
- Liu, Gang; Zhang, Jian; Bao, Jie
- Bioprocess and Biosystems Engineering, Vol. 39, Issue 1
Factors affecting the specificity of β-glucosidase from Fusarium oxysporum in enzymatic synthesis of alkyl-β-d-glucosides
journal, April 1998
- Makropoulou, M.; Christakopoulos, P.; Tsitsimpikou, C.
- International Journal of Biological Macromolecules, Vol. 22, Issue 2
A simple method to determine the water activity of ethanol-containing samples
journal, January 1999
- Hallsworth, J. E.; Nomura, Y.
- Biotechnology and Bioengineering, Vol. 62, Issue 2
Simultaneous Utilization of Cellobiose, Xylose, and Acetic Acid from Lignocellulosic Biomass for Biofuel Production by an Engineered Yeast Platform
journal, November 2014
- Wei, Na; Oh, Eun Joong; Million, Gyver
- ACS Synthetic Biology, Vol. 4, Issue 6
Lactose fermentation by engineered Saccharomyces cerevisiae capable of fermenting cellobiose
journal, September 2016
- Liu, Jing-Jing; Zhang, Guo-Chang; Oh, Eun Joong
- Journal of Biotechnology, Vol. 234
Simultaneous co-fermentation of mixed sugars: a promising strategy for producing cellulosic ethanol
journal, May 2012
- Kim, Soo Rin; Ha, Suk-Jin; Wei, Na
- Trends in Biotechnology, Vol. 30, Issue 5
Synergistic Saccharification, and Direct Fermentation to Ethanol, of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplaying Three Types of Cellulolytic Enzyme
journal, February 2004
- Fujita, Y.; Ito, J.; Ueda, M.
- Applied and Environmental Microbiology, Vol. 70, Issue 2, p. 1207-1212
The use of octyl β-d-glucoside as detergent for hog kidney brush border membrane
journal, October 1979
- Lin, J. T.; Riedel, S.; Kinne, R.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 557, Issue 1
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds
journal, April 1995
- Mumberg, Dominik; Müller, Rolf; Funk, Martin
- Gene, Vol. 156, Issue 1
Transglycosylation: A Key Reaction to Access Alkylpolyglycosides from Lignocellulosic Biomass
journal, April 2018
- Jérôme, François; Marinkovic, Sinisa; Estrine, Boris
- ChemSusChem, Vol. 11, Issue 9
The Techno-Economic Basis for Coproduct Manufacturing To Enable Hydrocarbon Fuel Production from Lignocellulosic Biomass
journal, May 2016
- Biddy, Mary J.; Davis, Ryan; Humbird, David
- ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 6
Mass spectrometry in the structural analysis of flavonoids
journal, January 2004
- Cuyckens, Filip; Claeys, Magda
- Journal of Mass Spectrometry, Vol. 39, Issue 1
Gene Amplification on Demand Accelerates Cellobiose Utilization in Engineered Saccharomyces cerevisiae
journal, April 2016
- Oh, Eun Joong; Skerker, Jeffrey M.; Kim, Soo Rin
- Applied and Environmental Microbiology, Vol. 82, Issue 12
Cellulose: Fascinating Biopolymer and Sustainable Raw Material
journal, May 2005
- Klemm, Dieter; Heublein, Brigitte; Fink, Hans-Peter
- Angewandte Chemie International Edition, Vol. 44, Issue 22
Effect of Reduction in Yeast and Enzyme Concentrations in a Simultaneous- Saccharification-and-Fermentation–Based Bioethanol Process: Technical and Economic Evaluation
journal, January 2005
- Wingren, Anders; Galbe, Mats; Roslander, Christian
- Applied Biochemistry and Biotechnology, Vol. 122, Issue 1-3
A review of cellulosic biofuel commercial-scale projects in the United States
journal, February 2013
- Brown, Tristan R.; Brown, Robert C.
- Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 3
Cellulose conversion into alkylglycosides in the ionic liquid 1-butyl-3-methylimidazolium chloride
journal, January 2010
- Ignatyev, Igor A.; Mertens, Pascal G. N.; Van Doorslaer, Charlie
- Green Chemistry, Vol. 12, Issue 10
β-Glucosidase catalyzed synthesis of octyl-β-d-glucopyranoside using whole cells of Pichia etchellsii in micro aqueous media
journal, December 2010
- Rather, Mohd. Younis; Mishra, Saroj; Chand, Subhash
- Journal of Biotechnology, Vol. 150, Issue 4
Glycosidases: a key to tailored carbohydrates
journal, April 2009
- Bojarová, Pavla; Křen, Vladimír
- Trends in Biotechnology, Vol. 27, Issue 4
Comparison of Conventional and Lignin-Rich Microcrystalline Cellulose
journal, February 2016
- Vanhatalo, Kari Mikael; Maximova, Natalia; Perander, Anna-Maija
- BioResources, Vol. 11, Issue 2
Purification and Characterization of an Extracellular β-Glucosidase II with High Hydrolysis and Transglucosylation Activities from Aspergillus niger
journal, February 1998
- Yan, Tsong-Rong; Lin, Yi-Hui; Lin, Chun-Leih
- Journal of Agricultural and Food Chemistry, Vol. 46, Issue 2
Derivation and synthesis of renewable surfactants
journal, January 2012
- Foley, Patrick; Kermanshahi pour, Azadeh; Beach, Evan S.
- Chem. Soc. Rev., Vol. 41, Issue 4
Biotransformation of methyl-β-d-glucopyranoside to higher chain alkyl glucosides by cell bound β-glucosidase of Pichia etchellsii
journal, March 2012
- Younis Rather, Mohd.; Mishra, Saroj; Verma, Vijeshwar
- Bioresource Technology, Vol. 107
Introduction of a Series of Alkyl Thiomaltosides, Useful New Non-Ionic Detergents, to Membrane Biochemistry1
journal, May 1993
- Izawa, Satoko; Sakai-Tomita, Yuki; Kinomura, Keisuke
- The Journal of Biochemistry, Vol. 113, Issue 5
Production of alkyl glucoside from cellooligosaccharides using yeast strains displaying Aspergillus aculeatus β-glucosidase 1
journal, November 2007
- Ito, Junji; Ebe, Takumi; Shibasaki, Seiji
- Journal of Molecular Catalysis B: Enzymatic, Vol. 49, Issue 1-4, p. 92-97
Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion
journal, December 2015
- Zhang, Guo-Chang; Liu, Jing-Jing; Kong, In Iok
- Current Opinion in Chemical Biology, Vol. 29
Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005
- Mosier, Nathan; Wyman, Charles; Dale, Bruce
- Bioresource Technology, Vol. 96, Issue 6, p. 673-686
Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process
journal, April 1993
- Philippidis, George P.; Smith, Tammy K.; Wyman, Charles E.
- Biotechnology and Bioengineering, Vol. 41, Issue 9, p. 846-853
Bio-ethanol – the fuel of tomorrow from the residues of today
journal, December 2006
- Hahn-Hägerdal, B.; Galbe, M.; Gorwa-Grauslund, M. F.
- Trends in Biotechnology, Vol. 24, Issue 12, p. 549-556
Microbial Cellulases and Their Industrial Applications
journal, January 2011
- Kuhad, Ramesh Chander; Gupta, Rishi; Singh, Ajay
- Enzyme Research, Vol. 2011
Glycosidase-catalysed synthesis of alkyl glycosides
journal, June 1999
- van Rantwijk, F.; Woudenberg-van Oosterom, M.; Sheldon, R. A.
- Journal of Molecular Catalysis B: Enzymatic, Vol. 6, Issue 6
Microbial production of fatty-acid-derived fuels and chemicals from plant biomass
journal, January 2010
- Steen, Eric J.; Kang, Yisheng; Bokinsky, Gregory
- Nature, Vol. 463, Issue 7280, p. 559-562
Ethyl glucoside in human urine following dietary exposure: detection by 1H NMR spectroscopy as a result of metabonomic screening of humans
journal, January 2004
- Teague, Claire; Holmes, Elaine; Maibaum, Elaine
- The Analyst, Vol. 129, Issue 3
Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels
journal, August 2013
- Balan, Venkatesh; Chiaramonti, David; Kumar, Sandeep
- Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 6
Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014
- Meng, Xianzhi; Ragauskas, Arthur Jonas
- Current Opinion in Biotechnology, Vol. 27
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
journal, December 2010
- Ha, S. -J.; Galazka, J. M.; Rin Kim, S.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 2
Structural Determination of Glucosylceramides in the Distillation Remnants of Shochu, the Japanese Traditional Liquor, and Its Production by Aspergillus kawachii
journal, November 2012
- Hirata, Miyo; Tsuge, Keisuke; Jayakody, Lahiru N.
- Journal of Agricultural and Food Chemistry, Vol. 60, Issue 46
Direct Conversion of Cellulose into Alkyl Glycoside Surfactants
journal, March 2017
- Puga, Alberto V.; Corma, Avelino
- ChemistrySelect, Vol. 2, Issue 8
Preparation of Long-Chain Alkyl Glucoside Surfactants by One-Step Direct Fischer Glucosidation, and by Transacetalation of Butyl Glucosides, on Beta Zeolite Catalysts
journal, December 1998
- Corma, A.; Iborra, S.; Miquel, S.
- Journal of Catalysis, Vol. 180, Issue 2
Cellulosic Biofuels--Got Gasoline?
journal, August 2009
- Regalbuto, J. R.
- Science, Vol. 325, Issue 5942
Outlook for cellulase improvement: Screening and selection strategies
journal, September 2006
- Percival Zhang, Y.-H.; Himmel, Michael E.; Mielenz, Jonathan R.
- Biotechnology Advances, Vol. 24, Issue 5, p. 452-481
Glucosylceramide Contained in Koji Mold-Cultured Cereal Confers Membrane and Flavor Modification and Stress Tolerance to Saccharomyces cerevisiae during Coculture Fermentation
journal, March 2015
- Sawada, Kazutaka; Sato, Tomoya; Hamajima, Hiroshi
- Applied and Environmental Microbiology, Vol. 81, Issue 11
Techno-Economic Evaluation of Producing Ethanol from Softwood: Comparison of SSF and SHF and Identification of Bottlenecks
journal, January 2003
- Wingren, Anders; Galbe, Mats; Zacchi, Guido
- Biotechnology Progress, Vol. 19, Issue 4
Optimization of enzyme complexes for lignocellulose hydrolysis
journal, January 2007
- Berlin, Alex; Maximenko, Vera; Gilkes, Neil
- Biotechnology and Bioengineering, Vol. 97, Issue 2, p. 287-296
Improved ethanol production by engineered Saccharomyces cerevisiae expressing a mutated cellobiose transporter during simultaneous saccharification and fermentation
journal, March 2017
- Lee, Won-Heong; Jin, Yong-Su
- Journal of Biotechnology, Vol. 245
Sugar-based surfactants for consumer products and technical applications
journal, January 1999
- Hill, Karlheinz; Rhode, Oliver
- Lipid - Fett, Vol. 101, Issue 1
Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production
journal, January 2013
- Singhania, Reeta Rani; Patel, Anil Kumar; Sukumaran, Rajeev K.
- Bioresource Technology, Vol. 127
Production of first and second generation biofuels: A comprehensive review
journal, February 2010
- Naik, S. N.; Goud, Vaibhav V.; Rout, Prasant K.
- Renewable and Sustainable Energy Reviews, Vol. 14, Issue 2
Efficient Production and Separation of Biodegradable Surfactants from Cellulose in 1-Butyl-3-Methylimidazolium Chloride
journal, October 2014
- Puga, Alberto V.; Corma, Avelino
- ChemSusChem, Vol. 7, Issue 12
Transformation of Cellulose into Biodegradable Alkyl Glycosides by Following Two Different Chemical Routes
journal, March 2011
- Villandier, Nicolas; Corma, Avelino
- ChemSusChem, Vol. 4, Issue 4
Foreign gene expression in yeast: a review
journal, June 1992
- Romanos, Michael A.; Scorer, Carol A.; Clare, Jeffrey J.
- Yeast, Vol. 8, Issue 6
Review of US and EU initiatives toward development, demonstration, and commercialization of lignocellulosic biofuels
journal, November 2013
- Balan, Venkatesh; Chiaramonti, David; Kumar, Sandeep
- Biofuels, Bioproducts and Biorefining, Vol. 7, Issue 6
Mass spectrometry in the structural analysis of flavonoids
journal, April 2004
- Cuyckens, Filip; Claeys, Magda
- Journal of Mass Spectrometry, Vol. 39, Issue 4
Cellulose: Fascinating Biopolymer and Sustainable Raw Material
journal, September 2005
- Klemm, Dieter; Heublein, Brigitte; Fink, Hans-Peter
- ChemInform, Vol. 36, Issue 36
Works referencing / citing this record:
Deficiency of β-Glucosidase Beneficial for the Simultaneous Saccharification and Lipid Production by the Oleaginous Yeast Lipomyces starkeyi
journal, September 2019
- Gou, Qingling; Tang, Mou; Wang, Yanan
- Applied Biochemistry and Biotechnology, Vol. 190, Issue 2
Selective radical depolymerization of cellulose to glucose induced by high frequency ultrasound
journal, January 2020
- Haouache, Somia; Karam, Ayman; Chave, Tony
- Chemical Science, Vol. 11, Issue 10