Protein hyperproduction in fungi by design
Abstract
The secretion of enzymes used by fungi to digest their environment has been exploited by humans for centuries for food and beverage production. More than a century after the first biotechnology patent, we know that the enzyme cocktails secreted by these amazing organisms have tremendous use across a number of industrial processes. Secreting the maximum titer of enzymes is critical to the economic feasibility of these processes. Traditional mutagenesis and screening approaches have generated the vast majority of strains used by industry for the production of enzymes. Until the emergence of economical next generation DNA sequencing platforms, the majority of the genes mutated in these screens remained uncharacterized at the sequence level. In addition, mutagenesis comes with a cost to an organism’s fitness, making tractable rational strain design approaches an attractive alternative. As an alternative to traditional utagenesis and screening, controlled manipulation of multiple genes involved in processes that impact the ability of a fungus to sense its environment, regulate transcription of enzyme-encoding genes, and efficiently secrete these proteins will allow for rational design of improved fungal protein production strains.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1463032
- Alternate Identifier(s):
- OSTI ID: 1468613; OSTI ID: 1513534
- Report Number(s):
- PNNL-SA-137094
Journal ID: ISSN 0175-7598; PII: 9265
- Grant/Contract Number:
- AC05-76RL01830; AC02-05CH11231; AC0576RL01830
- Resource Type:
- Published Article
- Journal Name:
- Applied Microbiology and Biotechnology
- Additional Journal Information:
- Journal Name: Applied Microbiology and Biotechnology; Journal ID: ISSN 0175-7598
- Publisher:
- Springer
- Country of Publication:
- Germany
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Enzyme; Protein; Hyperproduction; Secretion; Biodesign; Fungi; Biotechnology; Enzyme, Protein, Hyperproduction, Secretion, Biodesign, Fungi, Biotechnology
Citation Formats
Baker, Scott E. Protein hyperproduction in fungi by design. Germany: N. p., 2018.
Web. doi:10.1007/s00253-018-9265-1.
Baker, Scott E. Protein hyperproduction in fungi by design. Germany. https://doi.org/10.1007/s00253-018-9265-1
Baker, Scott E. Sat .
"Protein hyperproduction in fungi by design". Germany. https://doi.org/10.1007/s00253-018-9265-1.
@article{osti_1463032,
title = {Protein hyperproduction in fungi by design},
author = {Baker, Scott E.},
abstractNote = {The secretion of enzymes used by fungi to digest their environment has been exploited by humans for centuries for food and beverage production. More than a century after the first biotechnology patent, we know that the enzyme cocktails secreted by these amazing organisms have tremendous use across a number of industrial processes. Secreting the maximum titer of enzymes is critical to the economic feasibility of these processes. Traditional mutagenesis and screening approaches have generated the vast majority of strains used by industry for the production of enzymes. Until the emergence of economical next generation DNA sequencing platforms, the majority of the genes mutated in these screens remained uncharacterized at the sequence level. In addition, mutagenesis comes with a cost to an organism’s fitness, making tractable rational strain design approaches an attractive alternative. As an alternative to traditional utagenesis and screening, controlled manipulation of multiple genes involved in processes that impact the ability of a fungus to sense its environment, regulate transcription of enzyme-encoding genes, and efficiently secrete these proteins will allow for rational design of improved fungal protein production strains.},
doi = {10.1007/s00253-018-9265-1},
journal = {Applied Microbiology and Biotechnology},
number = ,
volume = ,
place = {Germany},
year = {Sat Aug 04 00:00:00 EDT 2018},
month = {Sat Aug 04 00:00:00 EDT 2018}
}
https://doi.org/10.1007/s00253-018-9265-1
Web of Science
Figures / Tables:
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