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Title: Protein hyperproduction in fungi by design

Abstract

The secretion of enzymes used by fungi to digest their environment has been exploited by humans for centuries for food and beverage production. More than a century after the first biotechnology patent, we know that the enzyme cocktails secreted by these amazing organisms have tremendous use across a number of industrial processes. Secreting the maximum titer of enzymes is critical to the economic feasibility of these processes. Traditional mutagenesis and screening approaches have generated the vast majority of strains used by industry for the production of enzymes. Until the emergence of economical next generation DNA sequencing platforms, the majority of the genes mutated in these screens remained uncharacterized at the sequence level. In addition, mutagenesis comes with a cost to an organism’s fitness, making tractable rational strain design approaches an attractive alternative. As an alternative to traditional utagenesis and screening, controlled manipulation of multiple genes involved in processes that impact the ability of a fungus to sense its environment, regulate transcription of enzyme-encoding genes, and efficiently secrete these proteins will allow for rational design of improved fungal protein production strains.

Authors:
ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1463032
Alternate Identifier(s):
OSTI ID: 1468613; OSTI ID: 1513534
Report Number(s):
PNNL-SA-137094
Journal ID: ISSN 0175-7598; PII: 9265
Grant/Contract Number:  
AC05-76RL01830; AC02-05CH11231; AC0576RL01830
Resource Type:
Published Article
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Name: Applied Microbiology and Biotechnology; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
Germany
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Enzyme; Protein; Hyperproduction; Secretion; Biodesign; Fungi; Biotechnology; Enzyme, Protein, Hyperproduction, Secretion, Biodesign, Fungi, Biotechnology

Citation Formats

Baker, Scott E. Protein hyperproduction in fungi by design. Germany: N. p., 2018. Web. doi:10.1007/s00253-018-9265-1.
Baker, Scott E. Protein hyperproduction in fungi by design. Germany. https://doi.org/10.1007/s00253-018-9265-1
Baker, Scott E. Sat . "Protein hyperproduction in fungi by design". Germany. https://doi.org/10.1007/s00253-018-9265-1.
@article{osti_1463032,
title = {Protein hyperproduction in fungi by design},
author = {Baker, Scott E.},
abstractNote = {The secretion of enzymes used by fungi to digest their environment has been exploited by humans for centuries for food and beverage production. More than a century after the first biotechnology patent, we know that the enzyme cocktails secreted by these amazing organisms have tremendous use across a number of industrial processes. Secreting the maximum titer of enzymes is critical to the economic feasibility of these processes. Traditional mutagenesis and screening approaches have generated the vast majority of strains used by industry for the production of enzymes. Until the emergence of economical next generation DNA sequencing platforms, the majority of the genes mutated in these screens remained uncharacterized at the sequence level. In addition, mutagenesis comes with a cost to an organism’s fitness, making tractable rational strain design approaches an attractive alternative. As an alternative to traditional utagenesis and screening, controlled manipulation of multiple genes involved in processes that impact the ability of a fungus to sense its environment, regulate transcription of enzyme-encoding genes, and efficiently secrete these proteins will allow for rational design of improved fungal protein production strains.},
doi = {10.1007/s00253-018-9265-1},
journal = {Applied Microbiology and Biotechnology},
number = ,
volume = ,
place = {Germany},
year = {Sat Aug 04 00:00:00 EDT 2018},
month = {Sat Aug 04 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1007/s00253-018-9265-1

Citation Metrics:
Cited by: 8 works
Citation information provided by
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Figures / Tables:

Fig. 1 Fig. 1: Manipulation of genes that encode proteins involved in regulating nutrient sensing, transcription, translation, and secretion is key for rational design of fungal lignocellulosic deconstruction enzyme hypersecretors

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