Title: Temperature sensitivity of extracellular enzymes differs with peat depth but not with season in an ombrotrophic bog

Abstract

Peatlands contain a large portion of Earth's terrestrial soil organic matter in part due to a reduction in decomposition rates. Organic matter decomposition is initially mediated by extracellular enzyme activity, which is in turn controlled by temperature, moisture, and substrate availability; and all are subject to seasonal variation. As depth increases in peatlands, temperature variability and labile carbon inputs decrease. We hypothesized that the more stable recalcitrant subsurface would contain a smaller less diverse enzyme pool, that is better adapted to a narrow temperature range. Thus temperature dependence would be diminished at depth compared to superficial peat. Potential enzyme activity rates were determined across seasons and with depth in peat samples collected from the Marcell Experimental Forest in northern Minnesota, USA. The temperature dependence, assessed by activation energy, was quantified for three hydrolytic enzymes involved in nutrient cycling at up to 15 temperature points ranging from 2 °C to 65 °C. Potential enzyme activity decreased with peat depth as expected and corresponded with changes in peat composition and microbial biomass from the acrotelm to the catotelm. In an environmentally relevant temperature range (2–23 °C), activation energy decreased with depth for β-glucosidase as predicted and leucine amino peptidase activation energy wasmore » the lowest of all enzymes. Stable temperatures at depth appear to result in a microbial community containing enzymes that have lower sensitivity to temperature increases. Surprisingly, there was no significant seasonal effect on enzyme temperature dependence observed in our study. Based on these results, and without shifts in microbial community composition, warming of peat could result in increased carbon and phosphorus cycling at the surface but little change at depth. As a result, differences in enzyme temperature sensitivity suggest nitrogen cycling could remain constant with warming, potentially resulting in proteolytic nitrogen cycling being decoupled from carbon and phosphorus cycling.« less

Authors:

Steinweg, Jessica Megan ^[1]; Kostka, Joel E. ^[2]; Hanson, Paul J.  ^[3];

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• Search DOE PAGES for ORCID "0000-0001-7293-3561"

Schadt, Christopher Warren  ^[4]

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• Search DOE PAGES for ORCID "0000-0001-8759-2448"

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+ Show Author Affiliations

1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Roanoke College, Salem, VA (United States)
2. Georgia Inst. of Technology, Atlanta, GA (United States)
3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Publication Date:

Thu Jul 26 00:00:00 EDT 2018

Research Org.:

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:

1462891

Alternate Identifier(s):

OSTI ID: 1548003

Grant/Contract Number:  

AC05-00OR22725; SC0012088

Resource Type:

Accepted Manuscript

Journal Name:

Soil Biology and Biochemistry

Additional Journal Information:

Journal Volume: 125; Journal Issue: C; Journal ID: ISSN 0038-0717

Publisher:

Elsevier

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; Peat depth; Extracellular enzyme activity; Decomposition; Nutrient cycling; Seasonality; Activation energy