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Title: Expression of Gre2p improves tolerance of engineered xylose-fermenting Saccharomyces cerevisiae to glycolaldehyde under xylose metabolism

Journal Article · · Applied Microbiology and Biotechnology
 [1];  [2];  [3];  [3];  [3];  [3]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Illinois, Urbana-Champaign, IL (United States)
  2. Univ. of Illinois, Urbana-Champaign, IL (United States); Northwestern Univ. Feinberg School of Medicine, Chicago, IL (United States)
  3. Univ. of Illinois, Urbana-Champaign, IL (United States)
+ Show Author Affiliations

Engineered S. cerevisiae employing the xylose reductase pathway enables efficient xylose valorization to fuels and chemicals. However, toxicity of thermochemically pretreated biomass hydrolysate on S. cerevisiae is one of the key technical challenges to upgrade biomass-derived sugars including xylose and glucose into high-value products. We investigated the effect of glycolaldehyde, one of the biomass-derived highly toxic aldehyde compounds, and its combinatorial inhibitory effect with other major fermentation inhibitors commonly found in plant hydrolysate such as methylglyoxal, 5-HMF, furfural, vanillin, and acetic acid on engineered xylose-fermenting S. cerevisiae in xylose and/or glucose media. We elucidated that glycolaldehyde and methylglyoxal are the key inhibitory short-aliphatic aldehydes on engineered xylose-fermenting S. cerevisiae in xylose-containing medium. Indeed, the degree of toxicity of these tested fermentation inhibitors varies with the sole carbon source of the medium. We demonstrate that genome integration of an extra copy of autologous GRE2 with its native promotor substantially improved the toxic tolerance of engineered xylose-fermenting S. cerevisiae to major inhibitory compounds including glycolaldehyde in the xylose-containing medium, and xylose-rich, lignocellulosic hydrolysate derived from Miscanthus giganteus, and concurrently improved the ethanol fermentation profile. In conclusion, outcomes of this study will aid the development of next-generation robust S. cerevisiae strains for efficient fermentation of hexose and pentose sugars found in biomass hydrolysate.

Research Organization:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1462470
Report Number(s):
NREL/JA--5100-72078
Journal Information:
Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology Journal Issue: 18 Vol. 102; ISSN 0175-7598
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (7)

Molecular and physiological basis of Saccharomyces cerevisiae tolerance to adverse lignocellulose-based process conditions journal November 2018
Engineered Saccharomyces cerevisiae for lignocellulosic valorization: a review and perspectives on bioethanol production journal January 2020
Sodium Acetate Responses in Saccharomyces cerevisiae and the Ubiquitin Ligase Rsp5 journal October 2018
Molecular and physiological basis of Saccharomyces cerevisiae tolerance to adverse lignocellulose-based process conditions journal November 2018
PKA and HOG signaling contribute separable roles to anaerobic xylose fermentation in yeast engineered for biofuel production posted_content February 2019
PKA and HOG signaling contribute separable roles to anaerobic xylose fermentation in yeast engineered for biofuel production journal May 2019
High Gravity Fermentation of Sugarcane Bagasse Hydrolysate by Saccharomyces pastorianus to Produce Economically Distillable Ethanol Concentrations: Necessity of Medium Components Examined journal January 2020

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09 BIOMASS FUELS
Gre2p
S. cerevisiae
aldehydes toxicity
glycolaldehyde
xylose