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Title: A Hypothetical Bottleneck in the Plant Microbiome

Abstract

The plant microbiome may be bottlenecked at the level of endophytes of individual seeds. Strong defense of developing seeds is predicted by optimal defense theory, and we have experimentally demonstrated exclusionary interactions among endophytic microbes infecting individual seeds of Centaurea stoebe. Having found a single, PDA-culturable microbe per seed or none in an exploratory study with Centaurea stoebe, we completed a more extensive survey of an additional 98 plant species representing 39 families. We again found that individual, surface-sterilized seeds of all species hosted only one PDA-culturable bacterial or fungal endophyte per seed, or none. PDA- unculturables were not determined but we expect them to also be bottlenecked in individual seeds, as they too should be governed by exclusionary interactions. If the bottleneck were confirmed with high-throughput sequencing of individual seeds then it would make sense to further investigate the Primary Symbiont Hypothesis (PSH). This includes the prediction that primary symbionts (i.e., the winners of the exclusionary battles among seed endophytes) have strong effects on seedlings depending on symbiont identity.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Idaho, Moscow, ID (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1462287
Alternate Identifier(s):
OSTI ID: 1510447
Grant/Contract Number:  
[SC0014547]
Resource Type:
Published Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
[Journal Name: Frontiers in Microbiology Journal Volume: 9]; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Media SA
Country of Publication:
Switzerland
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; seeds; maternal transmission; plant microbiome; priority effects; fungi; bacteria

Citation Formats

Newcombe, George, Harding, Abby, Ridout, Mary, and Busby, Posy E. A Hypothetical Bottleneck in the Plant Microbiome. Switzerland: N. p., 2018. Web. doi:10.3389/fmicb.2018.01645.
Newcombe, George, Harding, Abby, Ridout, Mary, & Busby, Posy E. A Hypothetical Bottleneck in the Plant Microbiome. Switzerland. doi:10.3389/fmicb.2018.01645.
Newcombe, George, Harding, Abby, Ridout, Mary, and Busby, Posy E. Tue . "A Hypothetical Bottleneck in the Plant Microbiome". Switzerland. doi:10.3389/fmicb.2018.01645.
@article{osti_1462287,
title = {A Hypothetical Bottleneck in the Plant Microbiome},
author = {Newcombe, George and Harding, Abby and Ridout, Mary and Busby, Posy E.},
abstractNote = {The plant microbiome may be bottlenecked at the level of endophytes of individual seeds. Strong defense of developing seeds is predicted by optimal defense theory, and we have experimentally demonstrated exclusionary interactions among endophytic microbes infecting individual seeds of Centaurea stoebe. Having found a single, PDA-culturable microbe per seed or none in an exploratory study with Centaurea stoebe, we completed a more extensive survey of an additional 98 plant species representing 39 families. We again found that individual, surface-sterilized seeds of all species hosted only one PDA-culturable bacterial or fungal endophyte per seed, or none. PDA- unculturables were not determined but we expect them to also be bottlenecked in individual seeds, as they too should be governed by exclusionary interactions. If the bottleneck were confirmed with high-throughput sequencing of individual seeds then it would make sense to further investigate the Primary Symbiont Hypothesis (PSH). This includes the prediction that primary symbionts (i.e., the winners of the exclusionary battles among seed endophytes) have strong effects on seedlings depending on symbiont identity.},
doi = {10.3389/fmicb.2018.01645},
journal = {Frontiers in Microbiology},
number = ,
volume = [9],
place = {Switzerland},
year = {2018},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.3389/fmicb.2018.01645

Citation Metrics:
Cited by: 1 work
Citation information provided by
Web of Science

Figures / Tables:

Table 1 Table 1: Proportion of seeds either without (i.e., ‘zero’) or with one or two PDA-culturable endophytes per surface-sterilized seed (70 seeds per species for 98 plant species from 39 families).

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    Figures / Tables found in this record:

      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.