Disentangling the complexity of permafrost soil by using high resolution profiling of microbial community composition, key functions and respiration rates: High resolution permafrost microbial profile

Müller, Oliver; Bang-Andreasen, Toke; White, Richard Allen; Elberling, Bo; Taş, Neslihan; Kneafsey, Timothy; Jansson, Janet K.; Øvreås, Lise

doi:10.1111/1462-2920.14348

Title: Disentangling the complexity of permafrost soil by using high resolution profiling of microbial community composition, key functions and respiration rates: High resolution permafrost microbial profile

Journal Article · 03 July 2018 · Environmental Microbiology

DOI: https://doi.org/10.1111/1462-2920.14348 · OSTI ID:1462122

^[1]; Bang-Andreasen, Toke ^[2]; White, Richard Allen ^[3]; Elberling, Bo ^[4]; Taş, Neslihan ^[5]; Kneafsey, Timothy ^[5]; Jansson, Janet K. ^[3]; Øvreås, Lise ^[6]

Bergen Univ. (Norway). Department of Biological Sciences
Aarhus University, Roskilde (Denmark). Department of Environmental Science; Copenhagen Univ. (Denmark). Department of Biology
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Copenhagen Univ. (Denmark). Department of Geosciences and Natural Resource Management, Center for Permafrost (CENPERM)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Bergen Univ. (Norway). Department of Biological Sciences; University Center in Svalbard, UNIS, Longyearbyen (Norway)

Thawing permafrost can stimulate microbial activity, leading to faster decomposition of formerly preserved organic matter and CO₂ release. Detailed knowledge about the vertical distribution of the responsible microbial community that is changing with increasing soil depth is limited. In this study, we determined the microbial community composition from cores sampled in a high Arctic heath at Svalbard, Norway; spanning from the active layer (AL) into the permafrost layer (PL). A special aim has been on identifying a layer of recently thawed soil, the transition zone (TZ), which might provide new insights into the fate of thawing permafrost. A unique sampling strategy allowed us to observe a diverse and gradually shifting microbial community in the AL, a Bacteroidetes dominated community in the TZ and throughout the PL, a community strongly dominated by a single Actinobacteria family (Intrasporangiaceae). The contrasting abundances of these two taxa caused a community difference of about 60%, just within 3 cm from TZ to PL. We incubated subsamples at about 5°C and measured highest CO₂ production rates under aerobic incubations, yet contrasting for five different layers and correlating to the microbial community composition. This high resolution strategy provides new insights on how microbial communities are structured in permafrost and a better understanding of how they respond to thaw.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830

OSTI ID:: 1462122

Alternate ID(s):: OSTI ID: 1462123; OSTI ID: 1476599; OSTI ID: 1496793

Report Number(s):: PNNL-SA-130170; ark:/13030/qt5mt359nr

Journal Information:: Environmental Microbiology, Vol. 20, Issue 12; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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