Widespread nitrogen fixation in sediments from diverse deep‐sea sites of elevated carbon loading

Dekas, Anne E.; Fike, David A.; Chadwick, Grayson L.; Green‐Saxena, Abigail; Fortney, Julian; Connon, Stephanie A.; Dawson, Katherine S.; Orphan, Victoria J.

doi:10.1111/1462-2920.14342

Title: Widespread nitrogen fixation in sediments from diverse deep‐sea sites of elevated carbon loading

Journal Article · 25 July 2018 · Environmental Microbiology

DOI: https://doi.org/10.1111/1462-2920.14342 · OSTI ID:1461897

^[1]; Fike, David A. ^[2]; Chadwick, Grayson L. ^[3]; Green‐Saxena, Abigail ^[3]; Fortney, Julian ^[4]; Connon, Stephanie A. ^[3]; Dawson, Katherine S. ^[3]; Orphan, Victoria J. ^[3]

Department of Earth System Science Stanford University Stanford CA 94305 USA, Division of Geological and Planetary Sciences California Institute of Technology Pasadena CA 91125 USA
Department of Earth &, Planetary Sciences Washington University in St. Louis St. Louis MO 63130 USA
Division of Geological and Planetary Sciences California Institute of Technology Pasadena CA 91125 USA
Department of Earth System Science Stanford University Stanford CA 94305 USA

Summary Nitrogen fixation, the biological conversion of N ₂ to NH ₃ , is critical to alleviating nitrogen limitation in many marine ecosystems. To date, few measurements exist of N ₂ fixation in deep‐sea sediments. Here, we conducted > 400 bottle incubations with sediments from methane seeps, whale falls and background sites off the western coast of the United States from 600 to 2893 m water depth to investigate the potential rates, spatial distribution and biological mediators of benthic N ₂ fixation. We found that N ₂ fixation was widespread, yet heterogeneously distributed with sediment depth at all sites. In some locations, rates exceeded previous measurements by > 10×, and provided up to 30% of the community anabolic growth requirement for nitrogen. Diazotrophic activity appeared to be inhibited by pore water ammonium: N ₂ fixation was only observed if incubation ammonium concentrations were ≤ 25 μM, and experimental additions of ammonium reduced diazotrophy. In seep sediments, N ₂ fixation was dependent on CH ₄ and coincident with sulphate reduction, consistent with previous work showing diazotrophy by microorganisms mediating sulphate‐coupled methane oxidation. However, the pattern of diazotrophy was different in whale‐fall and associated reference sediments, where it was largely unaffected by CH ₄ , suggesting catabolically different diazotrophs at these sites.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: NONE; SC0003940; SC0004949

OSTI ID:: 1461897

Alternate ID(s):: OSTI ID: 1610736

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 12 Vol. 20; ISSN 1462-2912

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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