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Title: Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells

Miniaturized multidimensional peptide separations can greatly increase the coverage in proteome profiling for trace samples.
Authors:
ORCiD logo [1] ; ORCiD logo [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [2] ; ORCiD logo [1] ; ORCiD logo [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Univ. of Florida, Gainesville, FL (United States)
Publication Date:
Report Number(s):
PNNL-SA-133217
Journal ID: ISSN 2041-6520; CSHCBM
Grant/Contract Number:
DP3DK110844; UC4DK104167; R33CA225248; R21EB020976; AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Chemical Science
Additional Journal Information:
Journal Name: Chemical Science; Journal ID: ISSN 2041-6520
Publisher:
Royal Society of Chemistry
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1461650