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Title: Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging

Abstract

Metal cofactors are required for many enzymes in anaerobic microbial respiration. This paper examined iron, cobalt, nickel, copper, and zinc in cellular and abiotic phases at the single-cell scale for a sulfate-reducing bacterium (Desulfococcus multivorans) and a methanogenic archaeon (Methanosarcina acetivorans) using synchrotron X-ray fluorescence microscopy. Relative abundances of cellular metals were also measured by inductively coupled plasma mass spectrometry. Finally, for both species, zinc and iron were consistently the most abundant cellular metals. M. acetivorans contained higher nickel and cobalt content than D. multivorans, likely due to elevated metal requirements for methylotrophic methanogenesis. Cocultures contained spheroid zinc sulfides and cobalt/copper sulfides.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3];  [1]; ORCiD logo [2]; ORCiD logo [1]; ORCiD logo [4]; ORCiD logo [3]
  1. Georgia Inst. of Technology, Atlanta, GA (United States). School of Earth and Atmospheric Sciences
  2. Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source
  3. California Inst. of Technology (CalTech), Pasadena, CA (United States). Division of Geological and Planetary Sciences
  4. Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States); California Inst. of Technology (CalTech), Pasadena, CA (United States); Georgia Inst. of Technology, Atlanta, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Aeronautic and Space Administration (NASA); National Science Foundation (NSF); Michigan Economic Development Corporation (United States); Michigan Technology Tri-Corridor (United States)
OSTI Identifier:
1461422
Grant/Contract Number:  
AC02-06CH11357; SC0004949; NNX14AJ87G; OCE-0939564; 085P1000817
Resource Type:
Accepted Manuscript
Journal Name:
Geomicrobiology Journal
Additional Journal Information:
Journal Volume: 35; Journal Issue: 1; Journal ID: ISSN 0149-0451
Publisher:
Taylor & Francis
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; metals; methanogen; methanol; sulfate reduction; synchrotron X-ray fluorescence

Citation Formats

Glass, Jennifer B., Chen, Si, Dawson, Katherine S., Horton, Damian R., Vogt, Stefan, Ingall, Ellery D., Twining, Benjamin S., and Orphan, Victoria J. Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging. United States: N. p., 2017. Web. doi:10.1080/01490451.2017.1321068.
Glass, Jennifer B., Chen, Si, Dawson, Katherine S., Horton, Damian R., Vogt, Stefan, Ingall, Ellery D., Twining, Benjamin S., & Orphan, Victoria J. Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging. United States. doi:10.1080/01490451.2017.1321068.
Glass, Jennifer B., Chen, Si, Dawson, Katherine S., Horton, Damian R., Vogt, Stefan, Ingall, Ellery D., Twining, Benjamin S., and Orphan, Victoria J. Fri . "Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging". United States. doi:10.1080/01490451.2017.1321068. https://www.osti.gov/servlets/purl/1461422.
@article{osti_1461422,
title = {Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging},
author = {Glass, Jennifer B. and Chen, Si and Dawson, Katherine S. and Horton, Damian R. and Vogt, Stefan and Ingall, Ellery D. and Twining, Benjamin S. and Orphan, Victoria J.},
abstractNote = {Metal cofactors are required for many enzymes in anaerobic microbial respiration. This paper examined iron, cobalt, nickel, copper, and zinc in cellular and abiotic phases at the single-cell scale for a sulfate-reducing bacterium (Desulfococcus multivorans) and a methanogenic archaeon (Methanosarcina acetivorans) using synchrotron X-ray fluorescence microscopy. Relative abundances of cellular metals were also measured by inductively coupled plasma mass spectrometry. Finally, for both species, zinc and iron were consistently the most abundant cellular metals. M. acetivorans contained higher nickel and cobalt content than D. multivorans, likely due to elevated metal requirements for methylotrophic methanogenesis. Cocultures contained spheroid zinc sulfides and cobalt/copper sulfides.},
doi = {10.1080/01490451.2017.1321068},
journal = {Geomicrobiology Journal},
number = 1,
volume = 35,
place = {United States},
year = {2017},
month = {4}
}

Journal Article:
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Figures / Tables:

Table 1 Table 1: Mean and standard error (in parentheses) of elemental contents normalized per cellular volume and per cell as measured by SXRF. Monocultures were prepared without chemical fixation, and cocultures were prepared with paraformaldehyde and glutaraldehyde fixation, followed by spotting onto silicon nitride wafers as described in the text. A,more » B and C superscripts indicate statistically different elemental contents (p < 0.05 based on Tukey-Kramer HSD test).« less

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    Works referencing / citing this record:

    Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging [Supplemental Data]
    dataset, May 2017

    • Glass, Jennifer; Chen, Si; Dawson, Katherine
    • figshare-Supplementary information for journal article at DOI: 10.1080/01490451.2017.1321068, 1 PDF file (837.71 kB)
    • DOI: 10.6084/m9.figshare.4900607

    Trace metal imaging of sulfate-reducing bacteria and methanogenic archaea at single-cell resolution by synchrotron X-ray fluorescence imaging [Supplemental Data]
    dataset, April 2017

    • Glass, Jennifer; Chen, Si; Dawson, Katherine
    • figshare-Supplementary information for journal article at DOI: 10.1080/01490451.2017.1321068, 1 PDF file (837.71 kB)
    • DOI: 10.6084/m9.figshare.4900607.v1

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