A sorghum NAC gene is associated with variation in biomass properties and yield potential
Abstract
Sorghum bicolor is a C4 grass widely cultivated for grain, forage, sugar, and biomass. The sorghum Dry Stalk (D) locus controls a qualitative difference between juicy green (dd) and dry white (D-) stalks and midribs, and co-localizes with a quantitative trait locus for sugar yield. Here, we apply fine-mapping and genome-wide association study (GWAS) to identify a candidate gene underlying D, and use nearly isogenic lines (NILs) to characterize the transcriptional, compositional, and agronomic effects of variation at the D locus. The D locus was fine-mapped to a 36 kb interval containing four genes. One of these genes is a NAC transcription factor that contains a stop codon in the NAC domain in the recessive (dd) parent. Allelic variation at D affects grain yield, sugar yield, and biomass composition in NILs. Green midrib (dd) NILs show reductions in lignin in stalk tissue and produce higher sugar and grain yields under well-watered field conditions. Increased yield potential in dd NILs is associated with increased stalk mass and moisture, higher biomass digestibility, and an extended period of grain filling. Transcriptome profiling of midrib tissue at the 4–6 leaf stages, when NILs first become phenotypically distinct, reveals that dd NILs have increased expressionmore »
- Authors:
-
- Department of Crop Sciences University of Illinois at Urbana Champaign Urbana Illinois
- Department of Plant and Microbial Biology University of California, Berkeley Berkeley California
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1461217
- Alternate Identifier(s):
- OSTI ID: 1461218; OSTI ID: 1575207; OSTI ID: 1575208
- Grant/Contract Number:
- DE‐SC0012400; SC0012400
- Resource Type:
- Published Article
- Journal Name:
- Plant Direct
- Additional Journal Information:
- Journal Name: Plant Direct Journal Volume: 2 Journal Issue: 7; Journal ID: ISSN 2475-4455
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biomass composition; biomass moisture; grain‐filling; secondary cell wall
Citation Formats
Xia, Jingnu, Zhao, Yunjun, Burks, Payne, Pauly, Markus, and Brown, Patrick J. A sorghum NAC gene is associated with variation in biomass properties and yield potential. United Kingdom: N. p., 2018.
Web. doi:10.1002/pld3.70.
Xia, Jingnu, Zhao, Yunjun, Burks, Payne, Pauly, Markus, & Brown, Patrick J. A sorghum NAC gene is associated with variation in biomass properties and yield potential. United Kingdom. https://doi.org/10.1002/pld3.70
Xia, Jingnu, Zhao, Yunjun, Burks, Payne, Pauly, Markus, and Brown, Patrick J. Mon .
"A sorghum NAC gene is associated with variation in biomass properties and yield potential". United Kingdom. https://doi.org/10.1002/pld3.70.
@article{osti_1461217,
title = {A sorghum NAC gene is associated with variation in biomass properties and yield potential},
author = {Xia, Jingnu and Zhao, Yunjun and Burks, Payne and Pauly, Markus and Brown, Patrick J.},
abstractNote = {Sorghum bicolor is a C4 grass widely cultivated for grain, forage, sugar, and biomass. The sorghum Dry Stalk (D) locus controls a qualitative difference between juicy green (dd) and dry white (D-) stalks and midribs, and co-localizes with a quantitative trait locus for sugar yield. Here, we apply fine-mapping and genome-wide association study (GWAS) to identify a candidate gene underlying D, and use nearly isogenic lines (NILs) to characterize the transcriptional, compositional, and agronomic effects of variation at the D locus. The D locus was fine-mapped to a 36 kb interval containing four genes. One of these genes is a NAC transcription factor that contains a stop codon in the NAC domain in the recessive (dd) parent. Allelic variation at D affects grain yield, sugar yield, and biomass composition in NILs. Green midrib (dd) NILs show reductions in lignin in stalk tissue and produce higher sugar and grain yields under well-watered field conditions. Increased yield potential in dd NILs is associated with increased stalk mass and moisture, higher biomass digestibility, and an extended period of grain filling. Transcriptome profiling of midrib tissue at the 4–6 leaf stages, when NILs first become phenotypically distinct, reveals that dd NILs have increased expression of a miniature zinc finger (MIF) gene. MIF genes dimerize with and suppress zinc finger homeodomain (ZF-HD) transcription factors, and a ZF-HD gene is associated with midrib color variation in a GWAS analysis across 1,694 diverse sorghum inbreds. A premature stop codon in a NAC gene is the most likely candidate polymorphism underlying the sorghum D locus. More detailed understanding of the sorghum D locus could help improve agronomic potential in cereals.},
doi = {10.1002/pld3.70},
journal = {Plant Direct},
number = 7,
volume = 2,
place = {United Kingdom},
year = {Mon Jul 23 00:00:00 EDT 2018},
month = {Mon Jul 23 00:00:00 EDT 2018}
}
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