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Title: Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors

Abstract

Zika virus detection was demonstrated using antibody-functionalized cover glasses externally connected to the gate electrode of an AlGaN/GaN high electron mobility transistor (HEMT). A pulsed bias voltage of 0.5 V was applied to an electrode on the region of the cover glass region functionalized with antibody, and the resulting changes of drain current of the HEMT were employed to determine the presence of Zika virus antigen concentration ranging from 0.1 to 100 ng/ml. The dynamic and static drain current changes as a function of Zika virus concentration were modeled with a spring-like elastic relaxation model and the Langmuir extension model, respectively. Excellent fits to the data were found with relaxation time constants of antibody and antigen molecules in the range of 11 μs and 0.66–24.4 μs, respectively, for the concentration range investigated. The ratio of antibody bound with antigen to the total available antibody on the functionalized contact window was in the range of 0.013–0.84 for the Zika antigen concentration range of 0.1–100 ng/ml. Since the HEMT is not exposed to the bio-solution, it can be used repeatedly. Here, the functionalized glass is the only disposable part in the detection system, showing the potential of this approach for hand-held, lowmore » cost sensor packages for point-of-care applications.« less

Authors:
 [1]; ORCiD logo [1]; ORCiD logo [1];  [2];  [1]; ORCiD logo [1]; ORCiD logo [3]
  1. Univ. of Florida, Gainesville, FL (United States)
  2. U.S. Naval Research Lab, Washington, D.C. (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1461056
Alternate Identifier(s):
OSTI ID: 1460502
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Applied Physics Letters
Additional Journal Information:
Journal Volume: 113; Journal Issue: 3; Journal ID: ISSN 0003-6951
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Yang, Jiancheng, Carey, IV, Patrick, Ren, Fan, Mastro, Michael A., Beers, Kimberly, Pearton, S. J., and Kravchenko, Ivan I. Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors. United States: N. p., 2018. Web. doi:10.1063/1.5029902.
Yang, Jiancheng, Carey, IV, Patrick, Ren, Fan, Mastro, Michael A., Beers, Kimberly, Pearton, S. J., & Kravchenko, Ivan I. Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors. United States. doi:10.1063/1.5029902.
Yang, Jiancheng, Carey, IV, Patrick, Ren, Fan, Mastro, Michael A., Beers, Kimberly, Pearton, S. J., and Kravchenko, Ivan I. Mon . "Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors". United States. doi:10.1063/1.5029902. https://www.osti.gov/servlets/purl/1461056.
@article{osti_1461056,
title = {Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors},
author = {Yang, Jiancheng and Carey, IV, Patrick and Ren, Fan and Mastro, Michael A. and Beers, Kimberly and Pearton, S. J. and Kravchenko, Ivan I.},
abstractNote = {Zika virus detection was demonstrated using antibody-functionalized cover glasses externally connected to the gate electrode of an AlGaN/GaN high electron mobility transistor (HEMT). A pulsed bias voltage of 0.5 V was applied to an electrode on the region of the cover glass region functionalized with antibody, and the resulting changes of drain current of the HEMT were employed to determine the presence of Zika virus antigen concentration ranging from 0.1 to 100 ng/ml. The dynamic and static drain current changes as a function of Zika virus concentration were modeled with a spring-like elastic relaxation model and the Langmuir extension model, respectively. Excellent fits to the data were found with relaxation time constants of antibody and antigen molecules in the range of 11 μs and 0.66–24.4 μs, respectively, for the concentration range investigated. The ratio of antibody bound with antigen to the total available antibody on the functionalized contact window was in the range of 0.013–0.84 for the Zika antigen concentration range of 0.1–100 ng/ml. Since the HEMT is not exposed to the bio-solution, it can be used repeatedly. Here, the functionalized glass is the only disposable part in the detection system, showing the potential of this approach for hand-held, low cost sensor packages for point-of-care applications.},
doi = {10.1063/1.5029902},
journal = {Applied Physics Letters},
number = 3,
volume = 113,
place = {United States},
year = {2018},
month = {7}
}

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