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Title: Tuning antimicrobial properties of biomimetic nanopatterned surfaces

Abstract

Here, nature has amassed an impressive array of structures that afford protection from microbial colonization/infection when displayed on the exterior surfaces of organisms. Here, controlled variation of the features of mimetics derived from etched silicon allows for tuning of their antimicrobial efficacy. Materials with nanopillars up to 7 μm in length are extremely effective against a wide range of microbial species and exceed the performance of natural surfaces; in contrast, materials with shorter/blunter nanopillars (<2 μm) selectively killed specific species. Using a combination of microscopies, the mechanisms by which bacteria are killed are demonstrated, emphasizing the dependence upon pillar density and tip geometry. Additionally, real-time imaging reveals how cells are immobilized and killed rapidly. Generic or selective protection from microbial colonization could be conferred to surfaces [for, e.g., internal medicine, implants (joint, dental, and cosmetic), food preparation, and the agricultural industry] patterned with these materials as coatings.

Authors:
ORCiD logo [1];  [1];  [1];  [2]; ORCiD logo [1];  [1]; ORCiD logo [1]
  1. Argonne National Lab. (ANL), Argonne, IL (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States); Pennsylvania State Univ., University Park, PA (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1460974
Alternate Identifier(s):
OSTI ID: 1434121
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Nanoscale
Additional Journal Information:
Journal Volume: 10; Journal Issue: 14; Journal ID: ISSN 2040-3364
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bactericidal; biomimetic surface; bioselectivity; black silicon; nanofabrication; nanotopography

Citation Formats

Michalska, Martyna, Gambacorta, Francesca, Divan, Ralu, Aranson, Igor S., Sokolov, Andrey, Noirot, Philippe, and Laible, Philip D. Tuning antimicrobial properties of biomimetic nanopatterned surfaces. United States: N. p., 2018. Web. doi:10.1039/c8nr00439k.
Michalska, Martyna, Gambacorta, Francesca, Divan, Ralu, Aranson, Igor S., Sokolov, Andrey, Noirot, Philippe, & Laible, Philip D. Tuning antimicrobial properties of biomimetic nanopatterned surfaces. United States. doi:10.1039/c8nr00439k.
Michalska, Martyna, Gambacorta, Francesca, Divan, Ralu, Aranson, Igor S., Sokolov, Andrey, Noirot, Philippe, and Laible, Philip D. Tue . "Tuning antimicrobial properties of biomimetic nanopatterned surfaces". United States. doi:10.1039/c8nr00439k. https://www.osti.gov/servlets/purl/1460974.
@article{osti_1460974,
title = {Tuning antimicrobial properties of biomimetic nanopatterned surfaces},
author = {Michalska, Martyna and Gambacorta, Francesca and Divan, Ralu and Aranson, Igor S. and Sokolov, Andrey and Noirot, Philippe and Laible, Philip D.},
abstractNote = {Here, nature has amassed an impressive array of structures that afford protection from microbial colonization/infection when displayed on the exterior surfaces of organisms. Here, controlled variation of the features of mimetics derived from etched silicon allows for tuning of their antimicrobial efficacy. Materials with nanopillars up to 7 μm in length are extremely effective against a wide range of microbial species and exceed the performance of natural surfaces; in contrast, materials with shorter/blunter nanopillars (<2 μm) selectively killed specific species. Using a combination of microscopies, the mechanisms by which bacteria are killed are demonstrated, emphasizing the dependence upon pillar density and tip geometry. Additionally, real-time imaging reveals how cells are immobilized and killed rapidly. Generic or selective protection from microbial colonization could be conferred to surfaces [for, e.g., internal medicine, implants (joint, dental, and cosmetic), food preparation, and the agricultural industry] patterned with these materials as coatings.},
doi = {10.1039/c8nr00439k},
journal = {Nanoscale},
number = 14,
volume = 10,
place = {United States},
year = {2018},
month = {3}
}

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Figures / Tables:

Fig. 1 Fig. 1: (a) The temporal evolution of nanopillar morphology (length and density) during the black silicon fabrication process spanning 1.5 to 30 min, and (b) schematic representation of resulting nanostructures. The etching rate is ca. 220 nm · min-1. The observed increase in nanopillar length over time is presented asmore » a cross view (main), and the corresponding nanopillar densities with scale-conserved base diameters as a top view on the surface of 1 μm2 (top). Total pillar length (L) is a sum of core plus tip ($L$tip). In the inset (right), a visualization of twin-pillars sharing a common base is shown, demonstrating how length and base diameter were measured in these cases. Note: when the etching time increases, the presence of these irregular structures also increases contributing to slight underestimation of pillar density at times > 20 min. Relevant sizes of the bacterial cells used for viability experiments are depicted (left; for details, see Table S1).« less

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