2'-O-methylation in mRNA disrupts tRNA decoding during translation elongation

Choi, Junhong; Indrisiunaite, Gabriele; DeMirci, Hasan; Ieong, Ka -Weng; Wang, Jinfan; Petrov, Alexey; Prabhakar, Arjun; Rechavi, Gideon; Dominissini, Dan; He, Chuan; Ehrenberg, Mans; Puglisi, Joseph D.

doi:10.1038/s41594-018-0030-z

Title: 2'-O-methylation in mRNA disrupts tRNA decoding during translation elongation

Journal Article · 19 February 2018 · Nature Structural & Molecular Biology

DOI: https://doi.org/10.1038/s41594-018-0030-z · OSTI ID:1460737

^[1]; Indrisiunaite, Gabriele ^[2];

^[3]; Ieong, Ka -Weng ^[2];

^[4]; Petrov, Alexey ^[5]; Prabhakar, Arjun ^[1]; Rechavi, Gideon ^[6]; Dominissini, Dan ^[6];

^[7]; Ehrenberg, Mans ^[2];

^[4]

Stanford Univ. School of Medicine, Stanford, CA (United States); Stanford Univ., Stanford, CA (United States)
Uppsala Univ., Uppsala (Sweden)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Stanford Univ. School of Medicine, Stanford, CA (United States)
Stanford Univ. School of Medicine, Stanford, CA (United States); Auburn Univ., Auburn, AL (United States)
Chaim Sheba Medical Center, Tel-Hashomer (Israel); Tel Aviv Univ., Tel Aviv (Israel)
The Univ. of Chicago, Chicago, IL (United States)

Chemical modifications of mRNA may regulate many aspects of mRNA processing and protein synthesis. Recently, 2'-O-methylation of nucleotides was identified as a frequent modification in translated regions of human mRNA, showing enrichment in codons for certain amino acids. Here, using single-molecule, bulk kinetics and structural methods, we show that 2'-O-methylation within coding regions of mRNA disrupts key steps in codon reading during cognate tRNA selection. Our results suggest that 2'-O-methylation sterically perturbs interactions of ribosomal-monitoring bases (G530, A1492 and A1493) with cognate codon–anticodon helices, thereby inhibiting downstream GTP hydrolysis by elongation factor Tu (EF-Tu) and A-site tRNA accommodation, leading to excessive rejection of cognate aminoacylated tRNAs in initial selection and proofreading. In conclusion, our current and prior findings highlight how chemical modifications of mRNA tune the dynamics of protein synthesis at different steps of translation elongation.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1460737

Journal Information:: Nature Structural & Molecular Biology, Vol. 25, Issue 3; ISSN 1545-9993

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 68 works

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