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Title: Characterization of plant carbon substrate utilization by Auxenochlorella protothecoides

Abstract

Algae hold great potential as a source of renewable fuel due to their ability to produce refinery-compatible diesel and jet fuel precursors. Significant effort has been made to maximize productivity under photoautotrophic growth conditions; however, little progress has been made to discover and understand reduced carbon assimilation pathways or enzymatic degradation of complex carbon substrates in algae. We purport that utilization of plant-based carbon substrates in addition to photosynthesis (mixotrophic growth) for biochemical assimilation into biomass, biofuels, and bioproducts, can increase cultivation productivity and improve the economic viability of algal-derived biofuels. Herein we report that a freshwater production strain of microalgae, Auxenochlorella protothecoides UTEX 25, is capable of directly degrading and utilizing non-food plant substrates, such as switchgrass, for cell growth. Glycome profiling of plant substrates before and after addition to A. protothecoides cultures demonstrates the utilization of xyloglucans. Genomic, proteomic and transcriptomic analyses revealed the identity of many enzymes that are hypothesized to be involved in complex carbohydrate degradation, including several family 5 and 9 glycosyl hydrolases. This work paves the way for future designer engineering of plant-carbon utilization to further improve productivity of algal production strains.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1460611
Alternate Identifier(s):
OSTI ID: 1463500; OSTI ID: 1466556
Report Number(s):
LA-UR-18-21621; NREL/JA-5100-72243
Journal ID: ISSN 2211-9264; S221192641830136X; PII: S221192641830136X
Grant/Contract Number:  
AC52-06NA25396; EE0003046; 20170533ECR; AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Algal Research
Additional Journal Information:
Journal Name: Algal Research Journal Volume: 34 Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Biological Science; algal biofuel; mixotrophic; plant substrate; genome

Citation Formats

Vogler, Brian W., Starkenburg, Shawn R., Sudasinghe, Nilusha, Schambach, Jenna Y., Rollin, Joseph A., Pattathil, Sivakumar, and Barry, Amanda N. Characterization of plant carbon substrate utilization by Auxenochlorella protothecoides. United States: N. p., 2018. Web. doi:10.1016/j.algal.2018.07.001.
Vogler, Brian W., Starkenburg, Shawn R., Sudasinghe, Nilusha, Schambach, Jenna Y., Rollin, Joseph A., Pattathil, Sivakumar, & Barry, Amanda N. Characterization of plant carbon substrate utilization by Auxenochlorella protothecoides. United States. https://doi.org/10.1016/j.algal.2018.07.001
Vogler, Brian W., Starkenburg, Shawn R., Sudasinghe, Nilusha, Schambach, Jenna Y., Rollin, Joseph A., Pattathil, Sivakumar, and Barry, Amanda N. Tue . "Characterization of plant carbon substrate utilization by Auxenochlorella protothecoides". United States. https://doi.org/10.1016/j.algal.2018.07.001.
@article{osti_1460611,
title = {Characterization of plant carbon substrate utilization by Auxenochlorella protothecoides},
author = {Vogler, Brian W. and Starkenburg, Shawn R. and Sudasinghe, Nilusha and Schambach, Jenna Y. and Rollin, Joseph A. and Pattathil, Sivakumar and Barry, Amanda N.},
abstractNote = {Algae hold great potential as a source of renewable fuel due to their ability to produce refinery-compatible diesel and jet fuel precursors. Significant effort has been made to maximize productivity under photoautotrophic growth conditions; however, little progress has been made to discover and understand reduced carbon assimilation pathways or enzymatic degradation of complex carbon substrates in algae. We purport that utilization of plant-based carbon substrates in addition to photosynthesis (mixotrophic growth) for biochemical assimilation into biomass, biofuels, and bioproducts, can increase cultivation productivity and improve the economic viability of algal-derived biofuels. Herein we report that a freshwater production strain of microalgae, Auxenochlorella protothecoides UTEX 25, is capable of directly degrading and utilizing non-food plant substrates, such as switchgrass, for cell growth. Glycome profiling of plant substrates before and after addition to A. protothecoides cultures demonstrates the utilization of xyloglucans. Genomic, proteomic and transcriptomic analyses revealed the identity of many enzymes that are hypothesized to be involved in complex carbohydrate degradation, including several family 5 and 9 glycosyl hydrolases. This work paves the way for future designer engineering of plant-carbon utilization to further improve productivity of algal production strains.},
doi = {10.1016/j.algal.2018.07.001},
journal = {Algal Research},
number = C,
volume = 34,
place = {United States},
year = {2018},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.algal.2018.07.001

Citation Metrics:
Cited by: 2 works
Citation information provided by
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Figures / Tables:

Fig. 1 Fig. 1: Algae utilization of carboxymethylcellulose (CMC) in the absence of light. Reduction of the red stain indicates CMC consumption. Prestained and stained plates of (A) A. protothecoides and (B) Chlamydomonas reinhardtii are shown.

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Works referencing / citing this record:

Secretion of Acetylxylan Esterase From Chlamydomonas reinhardtii Enables Utilization of Lignocellulosic Biomass as a Carbon Source
journal, February 2019

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