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Title: Programmed loading and rapid purification of engineered bacterial microcompartment shells

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Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States). DOE Plant Research Laboratory; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
Alternate Identifier(s):
OSTI ID: 1478349; OSTI ID: 1603659
Grant/Contract Number:  
FG02-91ER20021; AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Nature Communications
Additional Journal Information:
Journal Name: Nature Communications Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 2041-1723
Nature Publishing Group
Country of Publication:
United Kingdom

Citation Formats

Hagen, Andrew, Sutter, Markus, Sloan, Nancy, and Kerfeld, Cheryl A. Programmed loading and rapid purification of engineered bacterial microcompartment shells. United Kingdom: N. p., 2018. Web. doi:10.1038/s41467-018-05162-z.
Hagen, Andrew, Sutter, Markus, Sloan, Nancy, & Kerfeld, Cheryl A. Programmed loading and rapid purification of engineered bacterial microcompartment shells. United Kingdom. doi:10.1038/s41467-018-05162-z.
Hagen, Andrew, Sutter, Markus, Sloan, Nancy, and Kerfeld, Cheryl A. Mon . "Programmed loading and rapid purification of engineered bacterial microcompartment shells". United Kingdom. doi:10.1038/s41467-018-05162-z.
title = {Programmed loading and rapid purification of engineered bacterial microcompartment shells},
author = {Hagen, Andrew and Sutter, Markus and Sloan, Nancy and Kerfeld, Cheryl A.},
abstractNote = {},
doi = {10.1038/s41467-018-05162-z},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United Kingdom},
year = {2018},
month = {7}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1038/s41467-018-05162-z

Citation Metrics:
Cited by: 11 works
Citation information provided by
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Works referenced in this record:

Biogenesis of a Bacterial Organelle: The Carboxysome Assembly Pathway
journal, November 2013

A Simple Tagging System for Protein Encapsulation
journal, April 2006

  • Seebeck, Florian P.; Woycechowsky, Kenneth J.; Zhuang, Wei
  • Journal of the American Chemical Society, Vol. 128, Issue 14
  • DOI: 10.1021/ja058363s

A systems-level model reveals that 1,2-Propanediol utilization microcompartments enhance pathway flux through intermediate sequestration
journal, May 2017

  • Jakobson, Christopher M.; Tullman-Ercek, Danielle; Slininger, Marilyn F.
  • PLOS Computational Biology, Vol. 13, Issue 5
  • DOI: 10.1371/journal.pcbi.1005525

Stochastic reaction–diffusion simulation of enzyme compartmentalization reveals improved catalytic efficiency for a synthetic metabolic pathway
journal, July 2007

The Carboxysome Shell Is Permeable to Protons
journal, September 2010

  • Menon, B. B.; Heinhorst, S.; Shively, J. M.
  • Journal of Bacteriology, Vol. 192, Issue 22
  • DOI: 10.1128/JB.00903-10

A Catalytic Nanoreactor Based on in Vivo Encapsulation of Multiple Enzymes in an Engineered Protein Nanocompartment
journal, September 2016

Bacterial microcompartment assembly: The key role of encapsulation peptides
journal, May 2015

  • Aussignargues, Clément; Paasch, Bradley C.; Gonzalez-Esquer, Raul
  • Communicative & Integrative Biology, Vol. 8, Issue 3
  • DOI: 10.1080/19420889.2015.1039755

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%
journal, January 2012

  • Goedhart, Joachim; von Stetten, David; Noirclerc-Savoye, Marjolaine
  • Nature Communications, Vol. 3, Issue 1
  • DOI: 10.1038/ncomms1738

Molecular genetic analysis of a plant virus polyprotein cleavage site: A model
journal, August 1989

Microcompartments for B12-Dependent 1,2-Propanediol Degradation Provide Protection from DNA and Cellular Damage by a Reactive Metabolic Intermediate
journal, February 2008

  • Sampson, E. M.; Bobik, T. A.
  • Journal of Bacteriology, Vol. 190, Issue 8, p. 2966-2971
  • DOI: 10.1128/JB.01925-07

Engineering nanoscale protein compartments for synthetic organelles
journal, August 2013

Cyan and Yellow Super Fluorescent Proteins with Improved Brightness, Protein Folding, and FRET Förster Radius ,
journal, May 2006

  • Kremers, Gert-Jan; Goedhart, Joachim; van Munster, Erik B.
  • Biochemistry, Vol. 45, Issue 21
  • DOI: 10.1021/bi0516273

Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
journal, February 2012

  • Zakeri, B.; Fierer, J. O.; Celik, E.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 12
  • DOI: 10.1073/pnas.1115485109

Engineered Protein Nano-Compartments for Targeted Enzyme Localization
journal, March 2012

A Taxonomy of Bacterial Microcompartment Loci Constructed by a Novel Scoring Method
journal, October 2014

Short N-terminal sequences package proteins into bacterial microcompartments
journal, March 2010

  • Fan, C.; Cheng, S.; Liu, Y.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 16
  • DOI: 10.1073/pnas.0913199107

The Structure of CcmP, a Tandem Bacterial Microcompartment Domain Protein from the β-Carboxysome, Forms a Subcompartment Within a Microcompartment
journal, April 2013

  • Cai, Fei; Sutter, Markus; Cameron, Jeffrey C.
  • Journal of Biological Chemistry, Vol. 288, Issue 22
  • DOI: 10.1074/jbc.M113.456897

Assembly principles and structure of a 6.5-MDa bacterial microcompartment shell
journal, June 2017

The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle
journal, March 2016

Engineering Bacterial Microcompartment Shells: Chimeric Shell Proteins and Chimeric Carboxysome Shells
journal, July 2014

  • Cai, Fei; Sutter, Markus; Bernstein, Susan L.
  • ACS Synthetic Biology, Vol. 4, Issue 4
  • DOI: 10.1021/sb500226j

Ferritin, cellular iron storage and regulation: FERRITIN AND IRON STORAGE
journal, March 2017

  • Arosio, Paolo; Elia, Leonardo; Poli, Maura
  • IUBMB Life, Vol. 69, Issue 6
  • DOI: 10.1002/iub.1621

Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis
journal, March 2008

  • Parsons, Joshua B.; Dinesh, Sriramulu D.; Deery, Evelyne
  • Journal of Biological Chemistry, Vol. 283, Issue 21
  • DOI: 10.1074/jbc.M709214200

Assembly of Robust Bacterial Microcompartment Shells Using Building Blocks from an Organelle of Unknown Function
journal, May 2014

  • Lassila, Jonathan K.; Bernstein, Susan L.; Kinney, James N.
  • Journal of Molecular Biology, Vol. 426, Issue 11
  • DOI: 10.1016/j.jmb.2014.02.025

Bacterial microcompartments
journal, March 2018

  • Kerfeld, Cheryl A.; Aussignargues, Clement; Zarzycki, Jan
  • Nature Reviews Microbiology, Vol. 16, Issue 5
  • DOI: 10.1038/nrmicro.2018.10

Atomic-Level Models of the Bacterial Carboxysome Shell
journal, February 2008

Engineering nanoreactors using bacterial microcompartment architectures
journal, June 2018

Crystal structures of β-carboxysome shell protein CcmP: ligand binding correlates with the closed or open central pore
journal, March 2017

  • Larsson, Anna M.; Hasse, Dirk; Valegård, Karin
  • Journal of Experimental Botany, Vol. 68, Issue 14
  • DOI: 10.1093/jxb/erx070

A synthetic system for expression of components of a bacterial microcompartment
journal, September 2013

Molecular Interaction Between the Strep-tag Affinity Peptide and its Cognate Target, Streptavidin
journal, February 1996

  • Schmidt, Thomas G. M.; Koepke, Jürgen; Frank, Ronald
  • Journal of Molecular Biology, Vol. 255, Issue 5
  • DOI: 10.1006/jmbi.1996.0061

Identification and Structural Analysis of a Novel Carboxysome Shell Protein with Implications for Metabolite Transport
journal, September 2009

  • Klein, Michael G.; Zwart, Peter; Bagby, Sarah C.
  • Journal of Molecular Biology, Vol. 392, Issue 2
  • DOI: 10.1016/j.jmb.2009.03.056

De novo design of signal sequences to localize cargo to the 1,2-propanediol utilization microcompartment : Design of Signal Sequences
journal, April 2017

  • Jakobson, Christopher M.; Slininger Lee, Marilyn F.; Tullman-Ercek, Danielle
  • Protein Science, Vol. 26, Issue 5
  • DOI: 10.1002/pro.3144

Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998

Evidence for Improved Encapsulated Pathway Behavior in a Bacterial Microcompartment through Shell Protein Engineering
journal, June 2017

  • Slininger Lee, Marilyn F.; Jakobson, Christopher M.; Tullman-Ercek, Danielle
  • ACS Synthetic Biology, Vol. 6, Issue 10
  • DOI: 10.1021/acssynbio.7b00042

Protein Structures Forming the Shell of Primitive Bacterial Organelles
journal, August 2005

Elucidating Essential Role of Conserved Carboxysomal Protein CcmN Reveals Common Feature of Bacterial Microcompartment Assembly
journal, March 2012

  • Kinney, James N.; Salmeen, Annette; Cai, Fei
  • Journal of Biological Chemistry, Vol. 287, Issue 21
  • DOI: 10.1074/jbc.M112.355305

The Pentameric Vertex Proteins Are Necessary for the Icosahedral Carboxysome Shell to Function as a CO2 Leakage Barrier
journal, October 2009

The Distribution of Polyhedral Bacterial Microcompartments Suggests Frequent Horizontal Transfer and Operon Reassembly
journal, January 2013