Outer membrane vesicles for vaccination and targeted drug delivery

Wang, Sihan; Gao, Jin; Wang, Zhenjia

doi:10.1002/wnan.1523

Title: Outer membrane vesicles for vaccination and targeted drug delivery

Abstract

Extracellular vesicles (EVs) are cell membrane‐derived compartments that spontaneously secrete from a wide range of cells and tissues. EVs have shown to be the carriers in delivering drugs and small interfering RNA. Among EVs, bacterial outer membrane vesicles (OMVs) recently have gained the interest in vaccine development and targeted drug delivery. In this review, we summarize the current discoveries of OMVs and their functions. In particular, we focus on the biogenesis of OMVs and their functions in bacterial virulence and pathogenesis. Furthermore, we discuss the applications of OMVs in vaccination and targeted drug delivery. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Therapeutic Approaches and Drug Discovery > Emerging Technologies Biology‐Inspired Nanomaterials > Lipid‐Based Structures

Authors:

Wang, Sihan ^[1]; Gao, Jin ^[1]; Wang, Zhenjia ^[1]

Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences Washington State University Spokane Washington

Publication Date:: Thu Apr 26 00:00:00 EDT 2018

Sponsoring Org.:: USDOE

OSTI Identifier:: 1460579

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology

Additional Journal Information:: Journal Name: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology Journal Volume: 11 Journal Issue: 2; Journal ID: ISSN 1939-5116

Publisher:: Wiley Blackwell (John Wiley & Sons)

Country of Publication:: United States

Language:: English

Citation Formats


                    Wang, Sihan, Gao, Jin, and Wang, Zhenjia. Outer membrane vesicles for vaccination and targeted drug delivery.  United States: N. p., 2018. 
Web.  doi:10.1002/wnan.1523.

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                    Wang, Sihan, Gao, Jin, & Wang, Zhenjia. Outer membrane vesicles for vaccination and targeted drug delivery.  United States.  https://doi.org/10.1002/wnan.1523

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                    Wang, Sihan, Gao, Jin, and Wang, Zhenjia. Thu .  
"Outer membrane vesicles for vaccination and targeted drug delivery".  United States.  https://doi.org/10.1002/wnan.1523.

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@article{osti_1460579,

  title        = {Outer membrane vesicles for vaccination and targeted drug delivery},

  author       = {Wang, Sihan and Gao, Jin and Wang, Zhenjia},

  abstractNote = {Extracellular vesicles (EVs) are cell membrane‐derived compartments that spontaneously secrete from a wide range of cells and tissues. EVs have shown to be the carriers in delivering drugs and small interfering RNA. Among EVs, bacterial outer membrane vesicles (OMVs) recently have gained the interest in vaccine development and targeted drug delivery. In this review, we summarize the current discoveries of OMVs and their functions. In particular, we focus on the biogenesis of OMVs and their functions in bacterial virulence and pathogenesis. Furthermore, we discuss the applications of OMVs in vaccination and targeted drug delivery. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Therapeutic Approaches and Drug Discovery > Emerging Technologies Biology‐Inspired Nanomaterials > Lipid‐Based Structures},

  doi          = {10.1002/wnan.1523},

  journal      = {Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology},

  number       = 2,

  volume       = 11,

  place        = {United States},

  year         = {Thu Apr 26 00:00:00 EDT 2018},

  month        = {Thu Apr 26 00:00:00 EDT 2018}

}

Copy to clipboard

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Wang, Zhenjia; Malik, Asrar B.
Therapeutic Delivery, Vol. 4, Issue 2
DOI: 10.4155/tde.12.140

Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection
journal, October 2013

Bottero, D.; Gaillard, M. E.; Errea, A.
Vaccine, Vol. 31, Issue 45
DOI: 10.1016/j.vaccine.2013.08.059

Vesicle-Mediated Export and Assembly of Pore-Forming Oligomers of the Enterobacterial ClyA Cytotoxin
journal, October 2003

Wai, Sun Nyunt; Lindmark, Barbro; Söderblom, Tomas
Cell, Vol. 115, Issue 1
DOI: 10.1016/S0092-8674(03)00754-2

Recombinant outer membrane vesicles carrying Chlamydia muridarum HtrA induce antibodies that neutralize chlamydial infection in vitro
journal, January 2013

Bartolini, Erika; Ianni, Elvira; Frigimelica, Elisabetta
Journal of Extracellular Vesicles, Vol. 2, Issue 1
DOI: 10.3402/jev.v2i0.20181

Activated T cells recruit exosomes secreted by dendritic cells via LFA-1
journal, February 2009

Nolte-‘t Hoen, Esther N. M.; Buschow, Sonja I.; Anderton, Stephen M.
Blood, Vol. 113, Issue 9
DOI: 10.1182/blood-2008-08-174094

Modulating Antibacterial Immunity via Bacterial Membrane-Coated Nanoparticles
journal, January 2015

Gao, Weiwei; Fang, Ronnie H.; Thamphiwatana, Soracha
Nano Letters, Vol. 15, Issue 2
DOI: 10.1021/nl504798g

Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells
journal, November 2004

Kesty, Nicole C.; Mason, Kevin M.; Reedy, Mary
The EMBO Journal, Vol. 23, Issue 23
DOI: 10.1038/sj.emboj.7600471

Bioengineered Bacterial Outer Membrane Vesicles as Cell-Specific Drug-Delivery Vehicles for Cancer Therapy
journal, January 2014

Gujrati, Vipul; Kim, Sunghyun; Kim, Sang-Hyun
ACS Nano, Vol. 8, Issue 2
DOI: 10.1021/nn405724x

Helicobacter pylori Outer Membrane Vesicles Modulate Proliferation and Interleukin-8 Production by Gastric Epithelial Cells
journal, September 2003

Ismail, S.; Hampton, M. B.; Keenan, J. I.
Infection and Immunity, Vol. 71, Issue 10
DOI: 10.1128/IAI.71.10.5670-5675.2003

Nanoparticle biointerfacing by platelet membrane cloaking
journal, September 2015

Hu, Che-Ming J.; Fang, Ronnie H.; Wang, Kuei-Chun
Nature, Vol. 526, Issue 7571
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Target-responsive DNA/RNA nanomaterials for microRNA sensing and inhibition: The jack-of-all-trades in cancer nanotheranostics?
journal, January 2015

Conde, João; Edelman, Elazer R.; Artzi, Natalie
Advanced Drug Delivery Reviews, Vol. 81
DOI: 10.1016/j.addr.2014.09.003

Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice
journal, March 2016

Rappazzo, C. Garrett; Watkins, Hannah C.; Guarino, Cassandra M.
Vaccine, Vol. 34, Issue 10
DOI: 10.1016/j.vaccine.2016.01.028

Porphyromonas gingivalis Outer Membrane Vesicles Enter Human Epithelial Cells via an Endocytic Pathway and Are Sorted to Lysosomal Compartments
journal, August 2009

Furuta, N.; Tsuda, K.; Omori, H.
Infection and Immunity, Vol. 77, Issue 10
DOI: 10.1128/IAI.00009-09

Universal Vaccine Based on Ectodomain of Matrix Protein 2 of Influenza A: Fc Receptors and Alveolar Macrophages Mediate Protection
journal, December 2010

El Bakkouri, Karim; Descamps, Francis; De Filette, Marina
The Journal of Immunology, Vol. 186, Issue 2
DOI: 10.4049/jimmunol.0902147

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa
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Choi, Dong-Sic; Kim, Dae-Kyum; Choi, Seng Jin
PROTEOMICS, Vol. 11, Issue 16
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Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy
journal, August 2012

Lee, Y.; EL Andaloussi, S.; Wood, M. J. A.
Human Molecular Genetics, Vol. 21, Issue R1
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Vesiculation from Pseudomonas aeruginosa under SOS
journal, January 2012

Maredia, Reshma; Devineni, Navya; Lentz, Peter
The Scientific World Journal, Vol. 2012
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Preferential release of new outer membrane fragments by exponentially growing Escherichia coli
journal, April 1978

Mug-Opstelten, Davine; Witholt, Bernard
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 508, Issue 2
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Release of outer membrane fragments from normally growing Escherichia coli
journal, December 1976

Hoekstra, Dick; van der Laan, Jan Willem; de Leij, Loe
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 455, Issue 3
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Outer Membrane Vesicles (OMVs) of Gram-negative Bacteria: A Perspective Update
journal, June 2017

Jan, Arif Tasleem
Frontiers in Microbiology, Vol. 8
DOI: 10.3389/fmicb.2017.01053

Surface display of a borrelial lipoprotein on meningococcal outer membrane vesicles
journal, February 2016

Salverda, Merijn L. M.; Meinderts, Sanne M.; Hamstra, Hendrik-Jan
Vaccine, Vol. 34, Issue 8
DOI: 10.1016/j.vaccine.2016.01.019

Membrane vesicles are immunogenic facsimiles of Salmonella typhimurium that potently activate dendritic cells, prime B and T cell responses, and stimulate protective immunity in vivo
journal, February 2008

Alaniz, R. C.; Deatherage, B. L.; Lara, J. C.
The Journal of Immunology, Vol. 180, Issue 5
DOI: 10.4049/jimmunol.180.5.3612-b

Immunospecific Responses to Bacterial Elongation Factor Tu during Burkholderia Infection and Immunization
journal, December 2010

Nieves, Wildaliz; Heang, Julie; Asakrah, Saja
PLoS ONE, Vol. 5, Issue 12
DOI: 10.1371/journal.pone.0014361

Bacteriolytic effect of membrane vesicles from Pseudomonas aeruginosa on other bacteria including pathogens: conceptually new antibiotics.
journal, May 1996

Kadurugamuwa, J. L.; Beveridge, T. J.
Journal of Bacteriology, Vol. 178, Issue 10
DOI: 10.1128/jb.178.10.2767-2774.1996

Biological properties of extracellular vesicles and their physiological functions
journal, January 2015

Yáñez-Mó, María; Siljander, Pia R. -M.; Andreu, Zoraida
Journal of Extracellular Vesicles, Vol. 4, Issue 1
DOI: 10.3402/jev.v4.27066

Cell membrane-formed nanovesicles for disease-targeted delivery
journal, February 2016

Gao, Jin; Chu, Dafeng; Wang, Zhenjia
Journal of Controlled Release, Vol. 224
DOI: 10.1016/j.jconrel.2016.01.024

Gram-positive bacteria produce membrane vesicles: Proteomics-based characterization of Staphylococcus aureus-derived membrane vesicles
journal, December 2009

Lee, Eun-Young; Choi, Do-Young; Kim, Dae-Kyum
PROTEOMICS, Vol. 9, Issue 24
DOI: 10.1002/pmic.200900338

Principles of vaccine design—Lessons from nature
journal, August 2010

Zepp, Fred
Vaccine, Vol. 28
DOI: 10.1016/j.vaccine.2010.07.020

Human Lung Tissue Explants Reveal Novel Interactions during Legionella pneumophila Infections
journal, October 2013

Jäger, Jens; Marwitz, Sebastian; Tiefenau, Jana
Infection and Immunity, Vol. 82, Issue 1
DOI: 10.1128/IAI.00703-13

The Pseudomonas aeruginosa Lipid A Deacylase: Selection for Expression and Loss within the Cystic Fibrosis Airway
journal, December 2005

Ernst, R. K.; Adams, K. N.; Moskowitz, S. M.
Journal of Bacteriology, Vol. 188, Issue 1
DOI: 10.1128/JB.188.1.191-201.2006

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Haemophilus parasuis is a Gram-negative bacterium that colonizes the upper respiratory tract of swine and is capable of causing a systemic infection, resulting in high morbidity and mortality. H. parasuis isolates display a wide range of virulence and virulence factors are largely unknown. Commercial bacterins are often used to vaccinate swine against H. parasuis, though strain variability and lack of cross-reactivity can make this an ineffective means of protection. Outer membrane vesicles (OMV) are spherical structures naturally released from the membrane of bacteria and OMV are often enriched in toxins, signaling molecules and other bacterial components. Examination of OMV structuresmore »« less
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Title: Outer membrane vesicles for vaccination and targeted drug delivery

Abstract

Citation Formats

A Meningococcal Outer Membrane Vesicle Vaccine Incorporating Genetically Attenuated Endotoxin Dissociates Inflammation from Immunogenicity journal, December 2016

Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor journal, July 1983

Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles journal, April 2016

Enterotoxigenic Escherichia coli Secretes Active Heat-labile Enterotoxin via Outer Membrane Vesicles journal, April 2000

High yield, scalable and remotely drug-loaded neutrophil-derived extracellular vesicles (EVs) for anti-inflammation therapy journal, August 2017

Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi journal, September 2015

Size and Dynamics of Caveolae Studied Using Nanoparticles in Living Endothelial Cells journal, November 2009

Mechanisms of outer membrane vesicle entry into host cells: MicroReview - OMV entry into host cells journal, September 2016

Pulmonary Inflammation Induced by Bacteria-Free Outer Membrane Vesicles from Pseudomonas aeruginosa journal, October 2013

Salmonella outer membrane vesicles displaying high densities of pneumococcal antigen at the surface offer protection against colonization journal, April 2015

Immunization with Salmonella enterica Serovar Typhimurium-Derived Outer Membrane Vesicles Delivering the Pneumococcal Protein PspA Confers Protection against Challenge with Streptococcus pneumoniae journal, November 2010

Exosomes: immune properties and potential clinical implementations journal, December 2010

Release of a special fraction of the outer membrane from both growing and phage T4-infected Escherichia coli B journal, December 1978

Role of Toll-like receptor 5 in the innate immune response to acute P. aeruginosa pneumonia journal, December 2009

Inactivated and subunit vaccines to prevent shigellosis journal, December 2009

A novel meningococcal outer membrane vesicle vaccine with constitutive expression of FetA: A phase I clinical trial journal, September 2015

Vaccine Adjuvants: Putting Innate Immunity to Work journal, October 2010

Extracellular vesicles for drug delivery journal, November 2016

Outer-Membrane Vesicles Released by Normally Growing Escherichia coli Contain Very Little Lipoprotein journal, May 1981

Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen journal, January 2014

Entry of Porphyromonas gingivalis Outer Membrane Vesicles into Epithelial Cells Causes Cellular Functional Impairment journal, September 2009

Elastase and the LasA Protease of Pseudomonas aeruginosa Are Secreted with Their Propeptides journal, November 1998

Intranasal immunisation with inactivated RSV and bacterial adjuvants induces mucosal protection and abrogates eosinophilia upon challenge journal, May 2006

A Multi-Component Meningococcal Serogroup B Vaccine (4CMenB): The Clinical Development Program journal, December 2013

Bio-inspired, bioengineered and biomimetic drug delivery carriers journal, July 2011

Identification and optimization of critical process parameters for the production of NOMV vaccine against Neisseria meningitidis journal, May 2012

A holistic approach to targeting disease with polymeric nanoparticles journal, January 2015

Vesicle-Mediated Transfer of Virulence Genes from Escherichia coli O157:H7 to Other Enteric Bacteria journal, October 2000

Outer membrane-like vesicles secreted by Actinobacillus actinomycetemcomitans are enriched in leukotoxin journal, January 2002

Free-soluble and outer membrane vesicle-associated VacA from Helicobacter pylori: Two forms of release, a different activity journal, November 2005

Some Characteristics of the Outer Membrane Material Released by Growing Enterotoxigenic Escherichia coli journal, August 1980

Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response: Outer membrane vesicles relieve envelope stress journal, November 2006

Immunogenicity and safety of a hexavalent meningococcal outer-membrane-vesicle vaccine in children of 2–3 and 7–8 years of age journal, February 2000

Delivery of foreign antigens by engineered outer membrane vesicle vaccines journal, January 2010

Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticles journal, June 2004

Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response journal, August 2006

Nanoparticle Delivery of Cancer Drugs journal, February 2012

In vivo Kinetic Biodistribution of Nano-Sized Outer Membrane Vesicles Derived from Bacteria journal, September 2014

Immunogenicity and efficacy of oral or intranasal Shigella flexneri 2a and Shigella sonnei proteosome-lipopolysaccharide vaccines in animal models. journal, January 1993

Serum bactericidal activity and isotype distribution of antibodies in toddlers and schoolchildren after vaccination with RIVM hexavalent PorA vesicle vaccine journal, November 2001

Structural modifications of outer membrane vesicles to refine them as vaccine delivery vehicles journal, October 2009

Structure, Function, and Assembly of Cell Walls of Gram-Positive Bacteria journal, October 1983

Immunization with Escherichia coli Outer Membrane Vesicles Protects Bacteria - Induced Lethality via Th1 and Th17 Cell Responses journal, March 2013

Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell–cell contacts and induce histone H1 binding to ATP in epithelial cells journal, May 2015

Vaccines against meningococcal serogroup B disease containing outer membrane vesicles (OMV): Lessons from past programs and implications for the future journal, June 2013

Recent advances in pneumococcal peptidoglycan biosynthesis suggest new vaccine and antimicrobial targets journal, April 2012

Outer membrane vesicles as acellular vaccine against pertussis journal, August 2008

Identification of Proteins Associated with the Pseudomonas aeruginosa Biofilm Extracellular Matrix journal, September 2012

Delivery of nanoparticle-complexed drugs across the vascular endothelial barrier via caveolae journal, July 2011

Bacterial aminopeptidases: Properties and functions journal, July 1996

Virulence and Immunomodulatory Roles of Bacterial Outer Membrane Vesicles journal, March 2010

Neutrophil-Mediated Delivery of Therapeutic Nanoparticles across Blood Vessel Barrier for Treatment of Inflammation and Infection journal, October 2015

Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β 2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism journal, January 2015

Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms journal, August 2015

Bacterial Nanobioreactors–Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles journal, October 2015

Self-assembled micelles based on pH-sensitive PAE-g-MPEG-cholesterol block copolymer for anticancer drug delivery journal, October 2014

Anti-HER2 Treatment and Breast Cancer: State of the Art, Recent Patents, and New Strategies journal, January 2009

Lewis Epitopes on Outer Membrane Vesicles of Relevance to Helicobacter pylori Pathogenesis journal, April 2005

Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns journal, October 2010

Immune modulation by bacterial outer membrane vesicles journal, May 2015

A Novel Virulence Strategy for Pseudomonas aeruginosa Mediated by an Autotransporter with Arginine-Specific Aminopeptidase Activity journal, August 2012

Subtilase SprP exerts pleiotropic effects in Pseudomonas aeruginosa journal, December 2013

Decoration of Outer Membrane Vesicles with Multiple Antigens by Using an Autotransporter Approach journal, July 2014

DNA Is Packaged within Membrane-Derived Vesicles of Gram-Negative but Not Gram-Positive Bacteria journal, January 1990

Biogenesis of the Gram-Negative Bacterial Outer Membrane journal, October 2007

Horizontal Transfer of the OXA-24 Carbapenemase Gene via Outer Membrane Vesicles: a New Mechanism of Dissemination of Carbapenem Resistance Genes in Acinetobacter baumannii journal, April 2011

Structures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles journal, August 1999

Incorporation of Heterologous Outer Membrane and Periplasmic Proteins into Escherichia coli Outer Membrane Vesicles journal, October 2003

Outer membrane protein A (OmpA) from Shigella flexneri 2a: A promising subunit vaccine candidate journal, August 2013

Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis journal, June 2009

Uptake of Helicobacter pylori Outer Membrane Vesicles by Gastric Epithelial Cells journal, September 2010

Porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets journal, December 2000

Campylobacter jejuni Outer Membrane Vesicles Play an Important Role in Bacterial Interactions with Human Intestinal Epithelial Cells journal, September 2012

Long-Distance Delivery of Bacterial Virulence Factors by Pseudomonas aeruginosa Outer Membrane Vesicles journal, April 2009

The Flat-Ribbon Configuration of the Periplasmic Flagella of Borrelia burgdorferi and Its Relationship to Motility and Morphology journal, November 2008

Flagella proteins contribute to the production of outer membrane vesicles from Escherichia coli W3110 journal, November 2013

Comprehensive proteomic profiling of outer membrane vesicles from Campylobacter jejuni journal, February 2014

A Meningococcal Outer Membrane Vesicle Vaccine Incorporating Genetically Attenuated Endotoxin Dissociates Inflammation from Immunogenicity
journal, December 2016

Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor
journal, July 1983

Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles
journal, April 2016

Enterotoxigenic Escherichia coli Secretes Active Heat-labile Enterotoxin via Outer Membrane Vesicles
journal, April 2000

High yield, scalable and remotely drug-loaded neutrophil-derived extracellular vesicles (EVs) for anti-inflammation therapy
journal, August 2017

Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi
journal, September 2015

Size and Dynamics of Caveolae Studied Using Nanoparticles in Living Endothelial Cells
journal, November 2009

Mechanisms of outer membrane vesicle entry into host cells: MicroReview - OMV entry into host cells
journal, September 2016

Pulmonary Inflammation Induced by Bacteria-Free Outer Membrane Vesicles from Pseudomonas aeruginosa
journal, October 2013

Salmonella outer membrane vesicles displaying high densities of pneumococcal antigen at the surface offer protection against colonization
journal, April 2015

Immunization with Salmonella enterica Serovar Typhimurium-Derived Outer Membrane Vesicles Delivering the Pneumococcal Protein PspA Confers Protection against Challenge with Streptococcus pneumoniae
journal, November 2010

Exosomes: immune properties and potential clinical implementations
journal, December 2010

Release of a special fraction of the outer membrane from both growing and phage T4-infected Escherichia coli B
journal, December 1978

Role of Toll-like receptor 5 in the innate immune response to acute P. aeruginosa pneumonia
journal, December 2009

Inactivated and subunit vaccines to prevent shigellosis
journal, December 2009

A novel meningococcal outer membrane vesicle vaccine with constitutive expression of FetA: A phase I clinical trial
journal, September 2015

Vaccine Adjuvants: Putting Innate Immunity to Work
journal, October 2010

Extracellular vesicles for drug delivery
journal, November 2016

Outer-Membrane Vesicles Released by Normally Growing Escherichia coli Contain Very Little Lipoprotein
journal, May 1981

Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen
journal, January 2014

Entry of Porphyromonas gingivalis Outer Membrane Vesicles into Epithelial Cells Causes Cellular Functional Impairment
journal, September 2009

Elastase and the LasA Protease of Pseudomonas aeruginosa Are Secreted with Their Propeptides
journal, November 1998

Intranasal immunisation with inactivated RSV and bacterial adjuvants induces mucosal protection and abrogates eosinophilia upon challenge
journal, May 2006

A Multi-Component Meningococcal Serogroup B Vaccine (4CMenB): The Clinical Development Program
journal, December 2013

Bio-inspired, bioengineered and biomimetic drug delivery carriers
journal, July 2011

Identification and optimization of critical process parameters for the production of NOMV vaccine against Neisseria meningitidis
journal, May 2012

A holistic approach to targeting disease with polymeric nanoparticles
journal, January 2015

Vesicle-Mediated Transfer of Virulence Genes from Escherichia coli O157:H7 to Other Enteric Bacteria
journal, October 2000

Outer membrane-like vesicles secreted by Actinobacillus actinomycetemcomitans are enriched in leukotoxin
journal, January 2002

Free-soluble and outer membrane vesicle-associated VacA from Helicobacter pylori: Two forms of release, a different activity
journal, November 2005

Some Characteristics of the Outer Membrane Material Released by Growing Enterotoxigenic Escherichia coli
journal, August 1980

Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response: Outer membrane vesicles relieve envelope stress
journal, November 2006

Immunogenicity and safety of a hexavalent meningococcal outer-membrane-vesicle vaccine in children of 2–3 and 7–8 years of age
journal, February 2000

Delivery of foreign antigens by engineered outer membrane vesicle vaccines
journal, January 2010

Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticles
journal, June 2004

Purification of outer membrane vesicles from Pseudomonas aeruginosa and their activation of an IL-8 response
journal, August 2006

Nanoparticle Delivery of Cancer Drugs
journal, February 2012

In vivo Kinetic Biodistribution of Nano-Sized Outer Membrane Vesicles Derived from Bacteria
journal, September 2014

Immunogenicity and efficacy of oral or intranasal Shigella flexneri 2a and Shigella sonnei proteosome-lipopolysaccharide vaccines in animal models.
journal, January 1993

Serum bactericidal activity and isotype distribution of antibodies in toddlers and schoolchildren after vaccination with RIVM hexavalent PorA vesicle vaccine
journal, November 2001

Structural modifications of outer membrane vesicles to refine them as vaccine delivery vehicles
journal, October 2009

Structure, Function, and Assembly of Cell Walls of Gram-Positive Bacteria
journal, October 1983

Immunization with Escherichia coli Outer Membrane Vesicles Protects Bacteria - Induced Lethality via Th1 and Th17 Cell Responses
journal, March 2013

Helicobacter pylori vesicles carrying CagA localize in the vicinity of cell–cell contacts and induce histone H1 binding to ATP in epithelial cells
journal, May 2015

Vaccines against meningococcal serogroup B disease containing outer membrane vesicles (OMV): Lessons from past programs and implications for the future
journal, June 2013

Recent advances in pneumococcal peptidoglycan biosynthesis suggest new vaccine and antimicrobial targets
journal, April 2012

Outer membrane vesicles as acellular vaccine against pertussis
journal, August 2008

Identification of Proteins Associated with the Pseudomonas aeruginosa Biofilm Extracellular Matrix
journal, September 2012

Delivery of nanoparticle-complexed drugs across the vascular endothelial barrier via caveolae
journal, July 2011

Bacterial aminopeptidases: Properties and functions
journal, July 1996

Virulence and Immunomodulatory Roles of Bacterial Outer Membrane Vesicles
journal, March 2010

Neutrophil-Mediated Delivery of Therapeutic Nanoparticles across Blood Vessel Barrier for Treatment of Inflammation and Infection
journal, October 2015

Helicobacter pylori Outer Membrane Vesicle Proteins Induce Human Eosinophil Degranulation via a β 2 Integrin CD11/CD18- and ICAM-1-Dependent Mechanism
journal, January 2015

Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms
journal, August 2015

Bacterial Nanobioreactors–Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles
journal, October 2015

Self-assembled micelles based on pH-sensitive PAE-g-MPEG-cholesterol block copolymer for anticancer drug delivery
journal, October 2014

Anti-HER2 Treatment and Breast Cancer: State of the Art, Recent Patents, and New Strategies
journal, January 2009

Lewis Epitopes on Outer Membrane Vesicles of Relevance to Helicobacter pylori Pathogenesis
journal, April 2005

Vaccine delivery: a matter of size, geometry, kinetics and molecular patterns
journal, October 2010

Immune modulation by bacterial outer membrane vesicles
journal, May 2015

A Novel Virulence Strategy for Pseudomonas aeruginosa Mediated by an Autotransporter with Arginine-Specific Aminopeptidase Activity
journal, August 2012

Subtilase SprP exerts pleiotropic effects in Pseudomonas aeruginosa
journal, December 2013

Decoration of Outer Membrane Vesicles with Multiple Antigens by Using an Autotransporter Approach
journal, July 2014

DNA Is Packaged within Membrane-Derived Vesicles of Gram-Negative but Not Gram-Positive Bacteria
journal, January 1990

Biogenesis of the Gram-Negative Bacterial Outer Membrane
journal, October 2007

Horizontal Transfer of the OXA-24 Carbapenemase Gene via Outer Membrane Vesicles: a New Mechanism of Dissemination of Carbapenem Resistance Genes in Acinetobacter baumannii
journal, April 2011

Structures of Gram-Negative Cell Walls and Their Derived Membrane Vesicles
journal, August 1999

Incorporation of Heterologous Outer Membrane and Periplasmic Proteins into Escherichia coli Outer Membrane Vesicles
journal, October 2003

Outer membrane protein A (OmpA) from Shigella flexneri 2a: A promising subunit vaccine candidate
journal, August 2013

Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis
journal, June 2009

Uptake of Helicobacter pylori Outer Membrane Vesicles by Gastric Epithelial Cells
journal, September 2010

Porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets
journal, December 2000

Campylobacter jejuni Outer Membrane Vesicles Play an Important Role in Bacterial Interactions with Human Intestinal Epithelial Cells
journal, September 2012

Long-Distance Delivery of Bacterial Virulence Factors by Pseudomonas aeruginosa Outer Membrane Vesicles
journal, April 2009

The Flat-Ribbon Configuration of the Periplasmic Flagella of Borrelia burgdorferi and Its Relationship to Motility and Morphology
journal, November 2008

Flagella proteins contribute to the production of outer membrane vesicles from Escherichia coli W3110
journal, November 2013

Comprehensive proteomic profiling of outer membrane vesicles from Campylobacter jejuni
journal, February 2014

Virulence factors are released from Pseudomonas aeruginosa in association with membrane vesicles during normal growth and exposure to gentamicin: a novel mechanism of enzyme secretion.
journal, July 1995

Comparison of Intranasal Outer Membrane Vesicles with Cholera Toxin and Injected MF59C.1 as Adjuvants for Malaria Transmission Blocking Antigens AnAPN1 and Pfs48/45
journal, January 2016

Characterization of the osmotically inducible gene osmE of Escherichia coli K-12
journal, May 1995