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Title: Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus

Abstract

Here, Domain of Unknown Function 231-containing proteins (DUF231) are plant specific and their function is largely unknown. Studies in the model plants Arabidopsis and rice suggested that some DUF231 proteins act in the process of O-acetyl substitution of hemicellulose and esterification of pectin. However, little is known about the function of DUF231 proteins in woody plant species. This study provides evidence supporting that one member of DUF231 family proteins in the woody perennial plant Populus deltoides (genotype WV94), PdDUF231A, has a role in the acetylation of xylan and affects cellulose biosynthesis. A total of 52 DUF231-containing proteins were identified in the Populus genome. In P. deltoides transgenic lines overexpressing PdDUF231A ( OXPdDUF231A), glucose and cellulose contents were increased. Consistent with these results, the transcript levels of cellulose biosynthesis-related genes were increased in the OXPdDUF231A transgenic lines. Furthermore, the relative content of total acetylated xylan was increased in the OXPdDUF231A transgenic lines. Enzymatic saccharification assays revealed that the rate of glucose release increased in OXPdDUF231A transgenic lines. Plant biomass productivity was also increased in OXPdDUF231A transgenic lines. In conclusion, these results suggest that PdDUF231A affects cellulose biosynthesis and plays a role in the acetylation of xylan. PdDUF231A is a promising targetmore » for genetic modification for biofuel production because biomass productivity and compositional quality can be simultaneously improved through overexpression.« less

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2];  [2];  [2];  [1]; ORCiD logo [1];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [1]; ORCiD logo [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. ArborGen Inc., Ridgeville, SC (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1460243
Alternate Identifier(s):
OSTI ID: 1463990
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Acetylation; Cell wall; Cellulose; DUF231; Populus; Sugar release; Xylan

Citation Formats

Yang, Yongil, Yoo, Chang Geun, Winkeler, Kimberly A., Collins, Cassandra M., Hinchee, Maud A. W., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Pu, Yunqiao Joseph, Yang, Xiaohan, Tschaplinski, Timothy J., Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin -Gui. Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0998-3.
Yang, Yongil, Yoo, Chang Geun, Winkeler, Kimberly A., Collins, Cassandra M., Hinchee, Maud A. W., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Pu, Yunqiao Joseph, Yang, Xiaohan, Tschaplinski, Timothy J., Ragauskas, Arthur J., Tuskan, Gerald A., & Chen, Jin -Gui. Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus. United States. doi:10.1186/s13068-017-0998-3.
Yang, Yongil, Yoo, Chang Geun, Winkeler, Kimberly A., Collins, Cassandra M., Hinchee, Maud A. W., Jawdy, Sara S., Gunter, Lee E., Engle, Nancy L., Pu, Yunqiao Joseph, Yang, Xiaohan, Tschaplinski, Timothy J., Ragauskas, Arthur J., Tuskan, Gerald A., and Chen, Jin -Gui. Wed . "Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus". United States. doi:10.1186/s13068-017-0998-3. https://www.osti.gov/servlets/purl/1460243.
@article{osti_1460243,
title = {Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus},
author = {Yang, Yongil and Yoo, Chang Geun and Winkeler, Kimberly A. and Collins, Cassandra M. and Hinchee, Maud A. W. and Jawdy, Sara S. and Gunter, Lee E. and Engle, Nancy L. and Pu, Yunqiao Joseph and Yang, Xiaohan and Tschaplinski, Timothy J. and Ragauskas, Arthur J. and Tuskan, Gerald A. and Chen, Jin -Gui},
abstractNote = {Here, Domain of Unknown Function 231-containing proteins (DUF231) are plant specific and their function is largely unknown. Studies in the model plants Arabidopsis and rice suggested that some DUF231 proteins act in the process of O-acetyl substitution of hemicellulose and esterification of pectin. However, little is known about the function of DUF231 proteins in woody plant species. This study provides evidence supporting that one member of DUF231 family proteins in the woody perennial plant Populus deltoides (genotype WV94), PdDUF231A, has a role in the acetylation of xylan and affects cellulose biosynthesis. A total of 52 DUF231-containing proteins were identified in the Populus genome. In P. deltoides transgenic lines overexpressing PdDUF231A (OXPdDUF231A), glucose and cellulose contents were increased. Consistent with these results, the transcript levels of cellulose biosynthesis-related genes were increased in the OXPdDUF231A transgenic lines. Furthermore, the relative content of total acetylated xylan was increased in the OXPdDUF231A transgenic lines. Enzymatic saccharification assays revealed that the rate of glucose release increased in OXPdDUF231A transgenic lines. Plant biomass productivity was also increased in OXPdDUF231A transgenic lines. In conclusion, these results suggest that PdDUF231A affects cellulose biosynthesis and plays a role in the acetylation of xylan. PdDUF231A is a promising target for genetic modification for biofuel production because biomass productivity and compositional quality can be simultaneously improved through overexpression.},
doi = {10.1186/s13068-017-0998-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {12}
}

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Works referenced in this record:

MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004

  • Edgar, R. C.
  • Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
  • DOI: 10.1093/nar/gkh340