Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification
Abstract
Summary p ‐Coumaroyl ester 3‐hydroxylase (C3′H) is a key enzyme involved in the biosynthesis of lignin, a phenylpropanoid polymer that is the major constituent of secondary cell walls in vascular plants. Although the crucial role of C3′H in lignification and its manipulation to upgrade lignocellulose have been investigated in eudicots, limited information is available in monocotyledonous grass species, despite their potential as biomass feedstocks. Here we address the pronounced impacts of C3 ′ H deficiency on the structure and properties of grass cell walls. C3 ′ H ‐knockdown lines generated via RNA interference ( RNA i)‐mediated gene silencing, with about 0.5% of the residual expression levels, reached maturity and set seeds. In contrast, C3 ′ H ‐knockout rice mutants generated via CRISPR /Cas9‐mediated mutagenesis were severely dwarfed and sterile. Cell wall analysis of the mature C3 ′ H ‐knockdown RNA i lines revealed that their lignins were largely enriched in p ‐hydroxyphenyl (H) units while being substantially reduced in the normally dominant guaiacyl (G) and syringyl (S) units. Interestingly, however, the enrichment of H units was limited to within the non‐acylated lignin units, with grass‐specific γ‐ p ‐coumaroylated lignin units remaining apparently unchanged. Suppression of C3 ′ H also resultedmore »
- Authors:
-
- Kyoto Univ. (Japan). Research Inst. for Sustainable Humanosphere
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Tokushima Univ., Kuramoto-cho, Tokushima (Japan). Faculty of Bioscience and Bioindustry
- Kyoto Univ. (Japan). Graduate School of Agriculture
- Kyoto Univ. (Japan). Research Inst. for Sustainable Humanosphere; Kyoto Univ. (Japan). Research Unit for Development of Global Sustainability
- Publication Date:
- Research Org.:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); Japan Science and Technology Agency (JST); Japan International Cooperation Agency; Ministry of Agriculture, Forestry and Fisheries of Japan; Japan Society for the Promotion of Science (JSPS); New Energy and Industrial Technology Development Organization (NEDO)
- OSTI Identifier:
- 1459572
- Alternate Identifier(s):
- OSTI ID: 1459711
- Grant/Contract Number:
- SC0018409; FC02-07ER64494; DE‐FC02‐07ER64494; DE‐SC0018409
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Volume: 95; Journal Issue: 5; Journal ID: ISSN 0960-7412
- Publisher:
- Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; lignin; grass; Oryza sativa; RNAi; CRISPR/Cas9; saccharification; p-coumarate; ferulate; NMR; DFRC
Citation Formats
Takeda, Yuri, Tobimatsu, Yuki, Karlen, Steven D., Koshiba, Taichi, Suzuki, Shiro, Yamamura, Masaomi, Murakami, Shinya, Mukai, Mai, Hattori, Takefumi, Osakabe, Keishi, Ralph, John, Sakamoto, Masahiro, and Umezawa, Toshiaki. Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification. United States: N. p., 2018.
Web. doi:10.1111/tpj.13988.
Takeda, Yuri, Tobimatsu, Yuki, Karlen, Steven D., Koshiba, Taichi, Suzuki, Shiro, Yamamura, Masaomi, Murakami, Shinya, Mukai, Mai, Hattori, Takefumi, Osakabe, Keishi, Ralph, John, Sakamoto, Masahiro, & Umezawa, Toshiaki. Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification. United States. https://doi.org/10.1111/tpj.13988
Takeda, Yuri, Tobimatsu, Yuki, Karlen, Steven D., Koshiba, Taichi, Suzuki, Shiro, Yamamura, Masaomi, Murakami, Shinya, Mukai, Mai, Hattori, Takefumi, Osakabe, Keishi, Ralph, John, Sakamoto, Masahiro, and Umezawa, Toshiaki. Mon .
"Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification". United States. https://doi.org/10.1111/tpj.13988. https://www.osti.gov/servlets/purl/1459572.
@article{osti_1459572,
title = {Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification},
author = {Takeda, Yuri and Tobimatsu, Yuki and Karlen, Steven D. and Koshiba, Taichi and Suzuki, Shiro and Yamamura, Masaomi and Murakami, Shinya and Mukai, Mai and Hattori, Takefumi and Osakabe, Keishi and Ralph, John and Sakamoto, Masahiro and Umezawa, Toshiaki},
abstractNote = {Summary p ‐Coumaroyl ester 3‐hydroxylase (C3′H) is a key enzyme involved in the biosynthesis of lignin, a phenylpropanoid polymer that is the major constituent of secondary cell walls in vascular plants. Although the crucial role of C3′H in lignification and its manipulation to upgrade lignocellulose have been investigated in eudicots, limited information is available in monocotyledonous grass species, despite their potential as biomass feedstocks. Here we address the pronounced impacts of C3 ′ H deficiency on the structure and properties of grass cell walls. C3 ′ H ‐knockdown lines generated via RNA interference ( RNA i)‐mediated gene silencing, with about 0.5% of the residual expression levels, reached maturity and set seeds. In contrast, C3 ′ H ‐knockout rice mutants generated via CRISPR /Cas9‐mediated mutagenesis were severely dwarfed and sterile. Cell wall analysis of the mature C3 ′ H ‐knockdown RNA i lines revealed that their lignins were largely enriched in p ‐hydroxyphenyl (H) units while being substantially reduced in the normally dominant guaiacyl (G) and syringyl (S) units. Interestingly, however, the enrichment of H units was limited to within the non‐acylated lignin units, with grass‐specific γ‐ p ‐coumaroylated lignin units remaining apparently unchanged. Suppression of C3 ′ H also resulted in relative augmentation in tricin residues in lignin as well as a substantial reduction in wall cross‐linking ferulates. Collectively, our data demonstrate that C3 ′ H expression is an important determinant not only of lignin content and composition but also of the degree of cell wall cross‐linking. We also demonstrated that C3 ′ H ‐suppressed rice displays enhanced biomass saccharification.},
doi = {10.1111/tpj.13988},
journal = {The Plant Journal},
number = 5,
volume = 95,
place = {United States},
year = {Mon Jun 11 00:00:00 EDT 2018},
month = {Mon Jun 11 00:00:00 EDT 2018}
}
Web of Science
Figures / Tables:
Works referenced in this record:
Lignin Biosynthesis
journal, June 2003
- Boerjan, Wout; Ralph, John; Baucher, Marie
- Annual Review of Plant Biology, Vol. 54, Issue 1, p. 519-546
Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
journal, July 2015
- Fornalé, Silvia; Rencoret, Jorge; Garcia-Calvo, Laura
- Plant Science, Vol. 236
Tricin, a Flavonoid Monomer in Monocot Lignification
journal, February 2015
- Lan, Wu; Lu, Fachuang; Regner, Matthew
- Plant Physiology, Vol. 167, Issue 4
A polymer of caffeyl alcohol in plant seeds
journal, January 2012
- Chen, F.; Tobimatsu, Y.; Havkin-Frenkel, D.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 5
Feedstocks for Lignocellulosic Biofuels
journal, August 2010
- Somerville, Cris; Youngs, Heather; Taylor, Caroline
- Science, Vol. 329, Issue 5993, p. 790-792
Cell wall fermentation kinetics are impacted more by lignin content and ferulate cross-linking than by lignin composition
journal, January 2009
- Grabber, John H.; Mertens, David R.; Kim, Hoon
- Journal of the Science of Food and Agriculture, Vol. 89, Issue 1
Reductive Cleavage Method for Quantitation of Monolignols and Low-Abundance Monolignol Conjugates
journal, May 2018
- Regner, Matt; Bartuce, Allison; Padmakshan, Dharshana
- ChemSusChem, Vol. 11, Issue 10
Quantitative Molecular Structure–Pyrolytic Energy Correlation for Hardwood Lignins
journal, January 2012
- Silva, Teresa Cristina Fonseca; Santos, Ricardo Baillerini; Jameel, Hasan
- Energy & Fuels, Vol. 26, Issue 2
Changes in secondary metabolism and deposition of an unusual lignin in the ref8 mutant of Arabidopsis
journal, April 2002
- Franke, Rochus; Hemm, Matthew R.; Denault, Jeff W.
- The Plant Journal, Vol. 30, Issue 1, p. 47-59
Clustal W and Clustal X version 2.0
journal, September 2007
- Larkin, M. A.; Blackshields, G.; Brown, N. P.
- Bioinformatics, Vol. 23, Issue 21, p. 2947-2948
GOLD HULL AND INTERNODE2 Encodes a Primarily Multifunctional Cinnamyl-Alcohol Dehydrogenase in Rice
journal, January 2006
- Zhang, Kewei; Qian, Qian; Huang, Zejun
- Plant Physiology, Vol. 140, Issue 3
A comparison of the insoluble residues produced by the Klason lignin and acid detergent lignin procedures
journal, May 1994
- Hatfield, Ronald D.; Jung, Hans-Joachim G.; Ralph, John
- Journal of the Science of Food and Agriculture, Vol. 65, Issue 1, p. 51-58
Derivatization Followed by Reductive Cleavage (DFRC Method), a New Method for Lignin Analysis: Protocol for Analysis of DFRC Monomers
journal, July 1997
- Lu, Fachuang; Ralph, John
- Journal of Agricultural and Food Chemistry, Vol. 45, Issue 7
Loss of function of cinnamyl alcohol dehydrogenase 1 leads to unconventional lignin and a temperature-sensitive growth defect in Medicago truncatula
journal, July 2013
- Zhao, Q.; Tobimatsu, Y.; Zhou, R.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 33
Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility
journal, January 2018
- de Souza, Wagner R.; Martins, Polyana K.; Freeman, Jackie
- New Phytologist, Vol. 218, Issue 1
Down-regulation of four putative arabinoxylan feruloyl transferase genes from family PF02458 reduces ester-linked ferulate content in rice cell walls
journal, December 2009
- Piston, Fernando; Uauy, Cristobal; Fu, Lianhai
- Planta, Vol. 231, Issue 3
Convergent Evolution of Syringyl Lignin Biosynthesis via Distinct Pathways in the Lycophyte Selaginella and Flowering Plants
journal, April 2010
- Weng, Jing-Ke; Akiyama, Takuya; Bonawitz, Nicholas D.
- The Plant Cell, Vol. 22, Issue 4
Ferulate Cross-Links Limit the Enzymatic Degradation of Synthetically Lignified Primary Walls of Maize
journal, July 1998
- Grabber, John H.; Ralph, John; Hatfield, Ronald D.
- Journal of Agricultural and Food Chemistry, Vol. 46, Issue 7, p. 2609-2614
Can Lignin Be Accurately Measured?
journal, January 2005
- Hatfield, Ronald; Fukushima, Romualdo S.
- Crop Science, Vol. 45, Issue 3
Catalytic activity, duplication and evolution of the CYP98 cytochrome P450 family in wheat
journal, November 2006
- Morant, Marc; Schoch, Guillaume A.; Ullmann, Pascaline
- Plant Molecular Biology, Vol. 63, Issue 1
Identification of Grass-specific Enzyme That Acylates Monolignols with p-Coumarate
journal, January 2012
- Withers, Saunia; Lu, Fachuang; Kim, Hoon
- Journal of Biological Chemistry, Vol. 287, Issue 11, p. 8347-8355
Diferulate cross-links impede the enzymatic degradation of non-lignified maize walls
journal, June 1998
- Grabber, J. H.; Hatfield, R. D.; Ralph, J.
- Journal of the Science of Food and Agriculture, Vol. 77, Issue 2
High-throughput determination of thioglycolic acid lignin from rice
journal, January 2009
- Suzuki, Shiro; Suzuki, Yoshie; Yamamoto, Naoki
- Plant Biotechnology, Vol. 26, Issue 3
Downregulation of p-Coumaroyl Quinate/Shikimate 3′-Hydroxylase (C3′H) or Cinnamate-4-hydrolylase (C4H) in Eucalyptus urophylla × Eucalyptus grandis Leads to Increased Extractability
journal, June 2016
- Ziebell, Angela; Gjersing, Erica; Hinchee, Maud
- BioEnergy Research, Vol. 9, Issue 2
Preparative Thioacidolysis of Spruce Lignin: Isolation and Identification of Main Monomeric Products
journal, January 1986
- Lapierre, Catherine; Monties, Bernard; Rolando, Christian
- Holzforschung, Vol. 40, Issue 1
Phytozome: a comparative platform for green plant genomics
journal, November 2011
- Goodstein, David M.; Shu, Shengqiang; Howson, Russell
- Nucleic Acids Research, Vol. 40, Issue D1
CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants
journal, September 2014
- Lei, Yang; Lu, Li; Liu, Hai-Yang
- Molecular Plant, Vol. 7, Issue 9
Metabolic engineering of novel lignin in biomass crops
journal, October 2012
- Vanholme, Ruben; Morreel, Kris; Darrah, Chiarina
- New Phytologist, Vol. 196, Issue 4
Can genetic engineering of lignin deposition be accomplished without an unacceptable yield penalty?
journal, April 2013
- Bonawitz, Nicholas D.; Chapple, Clint
- Current Opinion in Biotechnology, Vol. 24, Issue 2
p -Coumaroyl-CoA:monolignol transferase (PMT) acts specifically in the lignin biosynthetic pathway in Brachypodium distachyon
journal, February 2014
- Petrik, Deborah L.; Karlen, Steven D.; Cass, Cynthia L.
- The Plant Journal, Vol. 77, Issue 5
Coexistence but Independent Biosynthesis of Catechyl and Guaiacyl/Syringyl Lignin Polymers in Seed Coats
journal, July 2013
- Tobimatsu, Y.; Chen, F.; Nakashima, J.
- The Plant Cell, Vol. 25, Issue 7
Syntheses of Lignin-Derived Thioacidolysis Monomers and Their Uses as Quantitation Standards
journal, January 2012
- Yue, Fengxia; Lu, Fachuang; Sun, Run-Cang
- Journal of Agricultural and Food Chemistry, Vol. 60, Issue 4
Highly Decorated Lignins in Leaf Tissues of the Canary Island Date Palm Phoenix canariensis
journal, September 2017
- Karlen, Steven D.; Smith, Rebecca A.; Kim, Hoon
- Plant Physiology, Vol. 175, Issue 3
NMR studies on lignocellulose deconstructions in the digestive system of the lower termite Coptotermes formosanus Shiraki
journal, January 2018
- Tarmadi, Didi; Tobimatsu, Yuki; Yamamura, Masaomi
- Scientific Reports, Vol. 8, Issue 1
Characterization of 5-Hydroxyconiferaldehyde O-Methyltransferase in Oryza sativa
journal, January 2013
- Koshiba, Taichi; Hirose, Norie; Mukai, Mai
- Plant Biotechnology, Vol. 30, Issue 2
Silencing of Hydroxycinnamoyl-Coenzyme A Shikimate/Quinate Hydroxycinnamoyltransferase Affects Phenylpropanoid Biosynthesis
journal, May 2004
- Hoffmann, Laurent; Besseau, Sébastien; Geoffroy, Pierrette
- The Plant Cell, Vol. 16, Issue 6
Genetic engineering of grass cell wall polysaccharides for biorefining
journal, June 2017
- Bhatia, Rakesh; Gallagher, Joe A.; Gomez, Leonardo D.
- Plant Biotechnology Journal, Vol. 15, Issue 9
Tricin-lignins: occurrence and quantitation of tricin in relation to phylogeny
journal, November 2016
- Lan, Wu; Rencoret, Jorge; Lu, Fachuang
- The Plant Journal, Vol. 88, Issue 6
Lignins: Natural polymers from oxidative coupling of 4-hydroxyphenyl- propanoids
journal, January 2004
- Ralph, John; Lundquist, Knut; Brunow, Gösta
- Phytochemistry Reviews, Vol. 3, Issue 1-2, p. 29-60
RiceXPro Version 3.0: expanding the informatics resource for rice transcriptome
journal, November 2012
- Sato, Yutaka; Takehisa, Hinako; Kamatsuki, Kaori
- Nucleic Acids Research, Vol. 41, Issue D1
Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014
- Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
- Science, Vol. 344, Issue 6185, p. 1246843-1246843
Down-regulation of p-coumaroyl quinate/shikimate 3′-hydroxylase (C3′H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla × E. grandis leads to improved sugar release
journal, August 2015
- Sykes, Robert W.; Gjersing, Erica L.; Foutz, Kirk
- Biotechnology for Biofuels, Vol. 8, Issue 1
The DFRC Method for Lignin Analysis. 6. A Simple Modification for Identifying Natural Acetates on Lignins
journal, November 1998
- Ralph, John; Lu, Fachuang
- Journal of Agricultural and Food Chemistry, Vol. 46, Issue 11, p. 4616-4619
Functional testing of a PF02458 homologue of putative rice arabinoxylan feruloyl transferase genes in Brachypodium distachyon
journal, November 2015
- Buanafina, Marcia M. de O.; Fescemyer, Howard W.; Sharma, Mandeep
- Planta, Vol. 243, Issue 3
Early lignin pathway enzymes and routes to chlorogenic acid in switchgrass (Panicum virgatum L.)
journal, November 2013
- Escamilla-Treviño, Luis L.; Shen, Hui; Hernandez, Timothy
- Plant Molecular Biology, Vol. 84, Issue 4-5
Grass Cell Walls: A Story of Cross-Linking
journal, January 2017
- Hatfield, Ronald D.; Rancour, David M.; Marita, Jane M.
- Frontiers in Plant Science, Vol. 7
Disrupting Flavone Synthase II Alters Lignin and Improves Biomass Digestibility
journal, April 2017
- Lam, Pui Ying; Tobimatsu, Yuki; Takeda, Yuri
- Plant Physiology, Vol. 174, Issue 2
Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
journal, July 2015
- Mikami, Masafumi; Toki, Seiichi; Endo, Masaki
- Plant Molecular Biology, Vol. 88, Issue 6
WWW-query: An on-line retrieval system for biological sequence banks
journal, January 1996
- Perrière, G.; Gouy, M.
- Biochimie, Vol. 78, Issue 5
Occurrence of Naturally Acetylated Lignin Units
journal, July 2007
- Del Río, José C.; Marques, Gisela; Rencoret, Jorge
- Journal of Agricultural and Food Chemistry, Vol. 55, Issue 14
RNAi-mediated suppression of p-coumaroyl-CoA 3'-hydroxylase in hybrid poplar impacts lignin deposition and soluble secondary metabolism
journal, March 2008
- Coleman, H. D.; Park, J. -Y.; Nair, R.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 11
Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells
journal, January 2011
- Yamamura, Masaomi; Wada, Shohei; Sakakibara, Norikazu
- Plant Biotechnology, Vol. 28, Issue 1
Functional Characterization of Evolutionarily Divergent 4-Coumarate:Coenzyme A Ligases in Rice
journal, August 2011
- Gui, Jinshan; Shen, Junhui; Li, Laigeng
- Plant Physiology, Vol. 157, Issue 2
Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis
journal, November 2014
- de Oliveira, Dyoni Matias; Finger-Teixeira, Aline; Rodrigues Mota, Thatiane
- Plant Biotechnology Journal, Vol. 13, Issue 9
The Arabidopsis REF8 gene encodes the 3-hydroxylase of phenylpropanoid metabolism
journal, April 2002
- Franke, Rochus; Humphreys, John M.; Hemm, Matthew R.
- The Plant Journal, Vol. 30, Issue 1, p. 33-45
The impact of alterations in lignin deposition on cellulose organization of the plant cell wall
journal, June 2016
- Liu, Jiliang; Kim, Jeong Im; Cusumano, Joanne C.
- Biotechnology for Biofuels, Vol. 9, Issue 1
Whole plant cell wall characterization using solution-state 2D NMR
journal, August 2012
- Mansfield, Shawn D.; Kim, Hoon; Lu, Fachuang
- Nature Protocols, Vol. 7, Issue 9
Monolignol ferulate conjugates are naturally incorporated into plant lignins
journal, October 2016
- Karlen, Steven D.; Zhang, Chengcheng; Peck, Matthew L.
- Science Advances, Vol. 2, Issue 10
Identification and suppression of the p‐ coumaroyl CoA:hydroxycinnamyl alcohol transferase in Zea mays L.
journal, May 2014
- Marita, Jane M.; Hatfield, Ronald D.; Rancour, David M.
- The Plant Journal, Vol. 78, Issue 5
The cinnamate/monolignol pathway
journal, November 2009
- Umezawa, Toshiaki
- Phytochemistry Reviews, Vol. 9, Issue 1
Microscale thioacidolysis method for the rapid analysis of ^|^#946;-O-4 substructures in lignin
journal, January 2012
- Yamamura, Masaomi; Hattori, Takefumi; Suzuki, Shiro
- Plant Biotechnology, Vol. 29, Issue 4
Decreased Polysaccharide Feruloylation Compromises Plant Cell Wall Integrity and Increases Susceptibility to Necrotrophic Fungal Pathogens
journal, May 2016
- Reem, Nathan T.; Pogorelko, Gennady; Lionetti, Vincenzo
- Frontiers in Plant Science, Vol. 7
Building the wall: recent advances in understanding lignin metabolism in grasses
journal, November 2016
- Cesarino, Igor; Simões, Marcella Siqueira; dos Santos Brito, Michael
- Acta Physiologiae Plantarum, Vol. 38, Issue 11
CAD2 deficiency causes both brown midrib and gold hull and internode phenotypes in Oryza sativa L. cv. Nipponbare
journal, January 2013
- Koshiba, Taichi; Murakami, Shinya; Hattori, Takefumi
- Plant Biotechnology, Vol. 30, Issue 4
A coumaroyl-ester-3-hydroxylase Insertion Mutant Reveals the Existence of Nonredundant meta -Hydroxylation Pathways and Essential Roles for Phenolic Precursors in Cell Expansion and Plant Growth
journal, December 2005
- Abdulrazzak, Nawroz; Pollet, Brigitte; Ehlting, Jürgen
- Plant Physiology, Vol. 140, Issue 1
Regulation of CONIFERALDEHYDE 5-HYDROXYLASE expression to modulate cell wall lignin structure in rice
journal, April 2017
- Takeda, Yuri; Koshiba, Taichi; Tobimatsu, Yuki
- Planta, Vol. 246, Issue 2
Effects of Coumarate 3-Hydroxylase Down-regulation on Lignin Structure
journal, January 2006
- Ralph, John; Akiyama, Takuya; Kim, Hoon
- Journal of Biological Chemistry, Vol. 281, Issue 13, p. 8843-8853
The world availability of non-wood lignocellulosic biomass for the production of cellulosic ethanol and potential pretreatments for the enhancement of enzymatic saccharification
journal, July 2016
- Tye, Ying Ying; Lee, Keat Teong; Wan Abdullah, Wan Nadiah
- Renewable and Sustainable Energy Reviews, Vol. 60
Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d6/pyridine-d5
journal, January 2010
- Kim, Hoon; Ralph, John
- Org. Biomol. Chem., Vol. 8, Issue 3
Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa (Medicago sativa L.)
journal, November 2005
- Reddy, M. S. S.; Chen, F.; Shadle, G.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 46
Paving the Way for Lignin Valorisation: Recent Advances in Bioengineering, Biorefining and Catalysis
journal, June 2016
- Rinaldi, Roberto; Jastrzebski, Robin; Clough, Matthew T.
- Angewandte Chemie International Edition, Vol. 55, Issue 29
CYP98A3 from Arabidopsis thaliana Is a 3′-Hydroxylase of Phenolic Esters, a Missing Link in the Phenylpropanoid Pathway
journal, June 2001
- Schoch, Guillaume; Goepfert, Simon; Morant, Marc
- Journal of Biological Chemistry, Vol. 276, Issue 39
Detection and Determination of p-Coumaroylated Units in Lignins
journal, May 1999
- Lu, Fachuang; Ralph, John
- Journal of Agricultural and Food Chemistry, Vol. 47, Issue 5, p. 1988-1992
Rapid analysis of transgenic rice straw using near-infrared spectroscopy
journal, January 2012
- Hattori, Takefumi; Murakami, Shinya; Mukai, Mai
- Plant Biotechnology, Vol. 29, Issue 4
Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014
- Bonawitz, Nicholas D.; Kim, Jeong Im; Tobimatsu, Yuki
- Nature, Vol. 509, Issue 7500, p. 376-380
MYB-mediated upregulation of lignin biosynthesis in <i>Oryza sativa</i> towards biomass refinery
journal, January 2017
- Koshiba, Taichi; Yamamoto, Naoki; Tobimatsu, Yuki
- Plant Biotechnology, Vol. 34, Issue 1
Effects on Lignin Structure of Coumarate 3-Hydroxylase Downregulation in Poplar
journal, May 2012
- Ralph, John; Akiyama, Takuya; Coleman, Heather D.
- BioEnergy Research, Vol. 5, Issue 4
Impact of engineered lignin composition on biomass recalcitrance and ionic liquid pretreatment efficiency
journal, January 2016
- Shi, Jian; Pattathil, Sivakumar; Parthasarathi, Ramakrishnan
- Green Chemistry, Vol. 18, Issue 18
Hydroxycinnamates in lignification
journal, August 2009
- Ralph, John
- Phytochemistry Reviews, Vol. 9, Issue 1, p. 65-83
Suppression of CINNAMOYL-CoA REDUCTASE increases the level of monolignol ferulates incorporated into maize lignins
journal, May 2017
- Smith, Rebecca A.; Cass, Cynthia L.; Mazaheri, Mona
- Biotechnology for Biofuels, Vol. 10, Issue 1
Upgrading of lignin-derived bio-oils by catalytic hydrodeoxygenation
journal, January 2014
- Saidi, Majid; Samimi, Fereshteh; Karimipourfard, Dornaz
- Energy Environ. Sci., Vol. 7, Issue 1
Hot alkali-labile linkages in the walls of the forage grass Phalaris aquatica and Lolium perenne and their relation to in vitro wall digestibility
journal, September 2003
- Lam, Thi Bach-Tuyet; Iiyama, Kenji; Stone, Bruce A.
- Phytochemistry, Vol. 64, Issue 2
A Systems Biology View of Responses to Lignin Biosynthesis Perturbations in Arabidopsis
journal, September 2012
- Vanholme, Ruben; Storme, Véronique; Vanholme, Bartel
- The Plant Cell, Vol. 24, Issue 9
Works referencing / citing this record:
Expression of Eucalyptus globulus LACCASE48 Restores Lignin Content of Arabidopsis thaliana lac17 Mutant
journal, November 2019
- Araújo, Pedro; Tolentino, Felipe Thadeu; Cesarino, Igor
- Plant Molecular Biology Reporter, Vol. 37, Issue 5-6
Os MYB 108 loss‐of‐function enriches p ‐coumaroylated and tricin lignin units in rice cell walls
journal, April 2019
- Miyamoto, Takuji; Takada, Rie; Tobimatsu, Yuki
- The Plant Journal
Low Lignin Mutants and Reduction of Lignin Content in Grasses for Increased Utilisation of Lignocellulose
journal, May 2019
- Christensen, Cecilie S. L.; Rasmussen, Søren K.
- Agronomy, Vol. 9, Issue 5
Recruitment of specific flavonoid B‐ring hydroxylases for two independent biosynthesis pathways of flavone‐derived metabolites in grasses
journal, April 2019
- Lam, Pui Ying; Lui, Andy C. W.; Yamamura, Masaomi
- New Phytologist, Vol. 223, Issue 1
4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase
journal, April 2019
- Barros, Jaime; Escamilla-Trevino, Luis; Song, Luhua
- Nature Communications, Vol. 10, Issue 1
OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls
journal, August 2019
- Lam, Pui Ying; Tobimatsu, Yuki; Matsumoto, Naoyuki
- Scientific Reports, Vol. 9, Issue 1
Lignin characterization of rice CONIFERALDEHYDE 5-HYDROXYLASE loss-of-function mutants generated with the CRISPR/Cas9 system
journal, December 2018
- Takeda, Yuri; Suzuki, Shiro; Tobimatsu, Yuki
- The Plant Journal, Vol. 97, Issue 3
Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice
journal, November 2019
- Martin, Andri Fadillah; Tobimatsu, Yuki; Kusumi, Ryosuke
- Scientific Reports, Vol. 9, Issue 1
Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition
journal, February 2019
- Takeda, Yuri; Tobimatsu, Yuki; Yamamura, Masaomi
- Journal of Wood Science, Vol. 65, Issue 1
Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency in Arabidopsis thaliana
journal, September 2018
- de Vries, Lisanne; Vanholme, Ruben; Van Acker, Rebecca
- Biotechnology for Biofuels, Vol. 11, Issue 1
4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase
journal, April 2019
- Barros, Jaime; Escamilla-Trevino, Luis; Song, Luhua
- Nature Communications, Vol. 10, Issue 1
OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls
journal, August 2019
- Lam, Pui Ying; Tobimatsu, Yuki; Matsumoto, Naoyuki
- Scientific Reports, Vol. 9, Issue 1
Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice
journal, November 2019
- Martin, Andri Fadillah; Tobimatsu, Yuki; Kusumi, Ryosuke
- Scientific Reports, Vol. 9, Issue 1
Figures / Tables found in this record: