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Title: Microbial Community Structure and Functional Potential Along a Hypersaline Gradient

Salinity is one of the strongest environmental drivers of microbial evolution and community composition. Here we aimed to determine the impact of salt concentrations (2.5, 7.5, and 33.2%) on the microbial community structure of reclaimed saltern ponds near San Francisco, California, and to discover prospective enzymes with potential biotechnological applications. Community compositions were determined by 16S rRNA amplicon sequencing revealing both higher richness and evenness in the pond sediments compared to the water columns. Co-occurrence network analysis additionally uncovered the presence of microbial seed bank communities, potentially primed to respond to rapid changes in salinity. In addition, functional annotation of shotgun metagenomic DNA showed different capabilities if the microbial communities at different salinities for methanogenesis, amino acid metabolism, and carbohydrate-active enzymes. There was an overall shift with increasing salinity in the functional potential for starch degradation, and a decrease in degradation of cellulose and other oligosaccharides. Further, many carbohydrate-active enzymes identified have acidic isoelectric points that have potential biotechnological applications, including deconstruction of biofuel feedstocks under high ionic conditions. Metagenome-assembled genomes (MAGs) of individual halotolerant and halophilic microbes were binned revealing a variety of carbohydrate-degrading potential of individual pond inhabitants.
Authors:
; ; ; ; ; ; ;
Publication Date:
Grant/Contract Number:
AC05-00OR22725; AC02-05CH11231; AC05-76RL01830
Type:
Published Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 9; Journal Issue: n/a; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
OSTI Identifier:
1459672
Alternate Identifier(s):
OSTI ID: 1471857

Kimbrel, Jeffrey A., Ballor, Nicholas, Wu, Yu-Wei, David, Maude M., Hazen, Terry C., Simmons, Blake A., Singer, Steven W., and Jansson, Janet K.. Microbial Community Structure and Functional Potential Along a Hypersaline Gradient. United States: N. p., Web. doi:10.3389/fmicb.2018.01492.
Kimbrel, Jeffrey A., Ballor, Nicholas, Wu, Yu-Wei, David, Maude M., Hazen, Terry C., Simmons, Blake A., Singer, Steven W., & Jansson, Janet K.. Microbial Community Structure and Functional Potential Along a Hypersaline Gradient. United States. doi:10.3389/fmicb.2018.01492.
Kimbrel, Jeffrey A., Ballor, Nicholas, Wu, Yu-Wei, David, Maude M., Hazen, Terry C., Simmons, Blake A., Singer, Steven W., and Jansson, Janet K.. 2018. "Microbial Community Structure and Functional Potential Along a Hypersaline Gradient". United States. doi:10.3389/fmicb.2018.01492.
@article{osti_1459672,
title = {Microbial Community Structure and Functional Potential Along a Hypersaline Gradient},
author = {Kimbrel, Jeffrey A. and Ballor, Nicholas and Wu, Yu-Wei and David, Maude M. and Hazen, Terry C. and Simmons, Blake A. and Singer, Steven W. and Jansson, Janet K.},
abstractNote = {Salinity is one of the strongest environmental drivers of microbial evolution and community composition. Here we aimed to determine the impact of salt concentrations (2.5, 7.5, and 33.2%) on the microbial community structure of reclaimed saltern ponds near San Francisco, California, and to discover prospective enzymes with potential biotechnological applications. Community compositions were determined by 16S rRNA amplicon sequencing revealing both higher richness and evenness in the pond sediments compared to the water columns. Co-occurrence network analysis additionally uncovered the presence of microbial seed bank communities, potentially primed to respond to rapid changes in salinity. In addition, functional annotation of shotgun metagenomic DNA showed different capabilities if the microbial communities at different salinities for methanogenesis, amino acid metabolism, and carbohydrate-active enzymes. There was an overall shift with increasing salinity in the functional potential for starch degradation, and a decrease in degradation of cellulose and other oligosaccharides. Further, many carbohydrate-active enzymes identified have acidic isoelectric points that have potential biotechnological applications, including deconstruction of biofuel feedstocks under high ionic conditions. Metagenome-assembled genomes (MAGs) of individual halotolerant and halophilic microbes were binned revealing a variety of carbohydrate-degrading potential of individual pond inhabitants.},
doi = {10.3389/fmicb.2018.01492},
journal = {Frontiers in Microbiology},
number = n/a,
volume = 9,
place = {United States},
year = {2018},
month = {7}
}

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