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This content will become publicly available on April 5, 2019

Title: Advances and prospects in metabolic engineering of Zymomonas mobilis

Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for commercial production of desirable bioproducts through metabolic engineering. In this paper, we summarize the metabolic engineering progress achieved in Z. mobilis to expand its substrate and product ranges as well as to enhance its robustness against stressful conditions such as inhibitory compounds within the lignocellulosic hydrolysates and slurries. We also discuss a few metabolic engineering strategies that can be applied in Z. mobilis to further develop it as a robust workhorse for economic lignocellulosic bioproducts. In addition, we briefly review the progress of metabolic engineering in Z. mobilis related to the classical synthetic biology cycle of “Design-Build-Test-Learn”, as well as the progress and potential to develop Z. mobilis as a model chassis for biorefinery practices in the synthetic biology era.
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [1] ;  [3] ;  [3] ;  [4] ;  [5] ;  [2] ;  [1]
  1. Hubei Univ., Wuhan (China). College of Life Sciences, Hubei Collaborative Innovation Center for Green Transformation of Bioresources, Environmental Microbial Technology Center and Hubei Key Lab. of Industrial Biotechnology
  2. Univ. of Texas, Austin, TX (United States). Cockrell School of Engineering, Dept. of Chemical Engineering and Inst. for Cellular and Molecular Biology
  3. Biogas Inst. of the Ministry of Agriculture (BIOMA), Chengdu (China). Biomass Energy Technology Research Centre and Key Lab. of Development and Application of Rural Renewable Energy
  4. Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center (GLBRC)
  5. East China Univ. of Science and Technology, Shanghai (China). State Key Lab. of Bioreactor Engineering
Publication Date:
Grant/Contract Number:
SC0018409; CBET-1254754; F-1756; DGE-1610403; FC02-07ER64494
Accepted Manuscript
Journal Name:
Metabolic Engineering
Additional Journal Information:
Journal Name: Metabolic Engineering; Journal ID: ISSN 1096-7176
Research Org:
Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center (GLBRC)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF); Welch Foundation
Country of Publication:
United States
09 BIOMASS FUELS; Zymomonas mobilis; Industrial chassis; Biorefinery; Biochemical production; Metabolic engineering; Synthetic biology
OSTI Identifier: