Advances and prospects in metabolic engineering of Zymomonas mobilis

Wang, Xia; He, Qiaoning; Yang, Yongfu; Wang, Jingwen; Haning, Katie; Hu, Yun; Wu, Bo; He, Mingxiong; Zhang, Yaoping; Bao, Jie; Contreras, Lydia M.; Yang, Shihui

doi:10.1016/j.ymben.2018.04.001

Title: Advances and prospects in metabolic engineering of Zymomonas mobilis

Abstract

Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for commercial production of desirable bioproducts through metabolic engineering. In this paper, we summarize the metabolic engineering progress achieved in Z. mobilis to expand its substrate and product ranges as well as to enhance its robustness against stressful conditions such as inhibitory compounds within the lignocellulosic hydrolysates and slurries. We also discuss a few metabolic engineering strategies that can be applied in Z. mobilis to further develop it as a robust workhorse for economic lignocellulosic bioproducts. In addition, we briefly review the progress of metabolic engineering in Z. mobilis related to the classical synthetic biology cycle of “Design-Build-Test-Learn”, as well as the progress and potential to develop Z. mobilis as a model chassis for biorefinery practices in the synthetic biology era.

Authors:

Wang, Xia ^[1]; He, Qiaoning ^[1]; Yang, Yongfu ^[1]; Wang, Jingwen ^[1]; Haning, Katie ^[2]; Hu, Yun ^[1]; Wu, Bo ^[3]; He, Mingxiong ^[3]; Zhang, Yaoping ^[4]; Bao, Jie ^[5]; Contreras, Lydia M. ^[2]; Yang, Shihui ^[1]

Hubei Univ., Wuhan (China). College of Life Sciences, Hubei Collaborative Innovation Center for Green Transformation of Bioresources, Environmental Microbial Technology Center and Hubei Key Lab. of Industrial Biotechnology
Univ. of Texas, Austin, TX (United States). Cockrell School of Engineering, Dept. of Chemical Engineering and Inst. for Cellular and Molecular Biology
Biogas Inst. of the Ministry of Agriculture (BIOMA), Chengdu (China). Biomass Energy Technology Research Centre and Key Lab. of Development and Application of Rural Renewable Energy
Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center (GLBRC)
East China Univ. of Science and Technology, Shanghai (China). State Key Lab. of Bioreactor Engineering

Publication Date:: Thu Apr 05 00:00:00 EDT 2018

Research Org.:: Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center (GLBRC)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Welch Foundation

OSTI Identifier:: 1459643

Alternate Identifier(s):: OSTI ID: 1819008

Grant/Contract Number:: SC0018409; CBET-1254754; F-1756; DGE-1610403; FC02-07ER64494; BER DE-FC02-07ER64494

Resource Type:: Accepted Manuscript

Journal Name:: Metabolic Engineering

Additional Journal Information:: Journal Volume: 50; Journal ID: ISSN 1096-7176

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; Zymomonas mobilis; Industrial chassis; Biorefinery; Biochemical production; Metabolic engineering; Synthetic biology

Citation Formats


                    Wang, Xia, He, Qiaoning, Yang, Yongfu, Wang, Jingwen, Haning, Katie, Hu, Yun, Wu, Bo, He, Mingxiong, Zhang, Yaoping, Bao, Jie, Contreras, Lydia M., and Yang, Shihui. Advances and prospects in metabolic engineering of Zymomonas mobilis.  United States: N. p., 2018. 
Web.  doi:10.1016/j.ymben.2018.04.001.

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                    Wang, Xia, He, Qiaoning, Yang, Yongfu, Wang, Jingwen, Haning, Katie, Hu, Yun, Wu, Bo, He, Mingxiong, Zhang, Yaoping, Bao, Jie, Contreras, Lydia M., & Yang, Shihui. Advances and prospects in metabolic engineering of Zymomonas mobilis.  United States.  https://doi.org/10.1016/j.ymben.2018.04.001

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                    Wang, Xia, He, Qiaoning, Yang, Yongfu, Wang, Jingwen, Haning, Katie, Hu, Yun, Wu, Bo, He, Mingxiong, Zhang, Yaoping, Bao, Jie, Contreras, Lydia M., and Yang, Shihui. Thu .  
"Advances and prospects in metabolic engineering of Zymomonas mobilis".  United States.  https://doi.org/10.1016/j.ymben.2018.04.001.  https://www.osti.gov/servlets/purl/1459643.

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@article{osti_1459643,

  title        = {Advances and prospects in metabolic engineering of Zymomonas mobilis},

  author       = {Wang, Xia and He, Qiaoning and Yang, Yongfu and Wang, Jingwen and Haning, Katie and Hu, Yun and Wu, Bo and He, Mingxiong and Zhang, Yaoping and Bao, Jie and Contreras, Lydia M. and Yang, Shihui},

  abstractNote = {Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen with many desirable characteristics, which makes it an ideal industrial microbial biocatalyst for commercial production of desirable bioproducts through metabolic engineering. In this paper, we summarize the metabolic engineering progress achieved in Z. mobilis to expand its substrate and product ranges as well as to enhance its robustness against stressful conditions such as inhibitory compounds within the lignocellulosic hydrolysates and slurries. We also discuss a few metabolic engineering strategies that can be applied in Z. mobilis to further develop it as a robust workhorse for economic lignocellulosic bioproducts. In addition, we briefly review the progress of metabolic engineering in Z. mobilis related to the classical synthetic biology cycle of “Design-Build-Test-Learn”, as well as the progress and potential to develop Z. mobilis as a model chassis for biorefinery practices in the synthetic biology era.},

  doi          = {10.1016/j.ymben.2018.04.001},

  journal      = {Metabolic Engineering},

  number       = ,

  volume       = 50,

  place        = {United States},

  year         = {Thu Apr 05 00:00:00 EDT 2018},

  month        = {Thu Apr 05 00:00:00 EDT 2018}

}

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Works referencing / citing this record:

Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering
journal, October 2019

Zheng, Yanli; Han, Jiamei; Wang, Baiyang
Nucleic Acids Research, Vol. 47, Issue 21
DOI: 10.1093/nar/gkz940

Applying a New REFINE Approach in Zymomonas mobilis Identifies Novel sRNAs That Confer Improved Stress Tolerance Phenotypes
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Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering
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Nucleic Acids Research, Vol. 47, Issue 21
DOI: 10.1093/nar/gkz940

Engineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis
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Wu, Bo; Qin, Han; Yang, Yiwei
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Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era
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Yang, Yongfu; Shen, Wei; Huang, Ju
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A Markerless Method for Genome Engineering in Zymomonas mobilis ZM4
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Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case of Zymomonas mobilis
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Kalnenieks, Uldis; Balodite, Elina; Rutkis, Reinis
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DOI: 10.3389/fbioe.2019.00327

A Markerless Method for Genome Engineering in Zymomonas mobilis ZM4
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Transcriptomic Profiles of Zymomonas mobilis 8b to Furfural Acute and Long-Term Stress in Both Glucose and Xylose Conditions
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Yang, Shihui; Franden, Mary Ann; Wang, Xia
Frontiers in Microbiology, Vol. 11
DOI: 10.3389/fmicb.2020.00013

Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis
journal, October 2019

Shen, Wei; Zhang, Jun; Geng, Binan
Microbial Cell Factories, Vol. 18, Issue 1
DOI: 10.1186/s12934-019-1219-5

Identification and characterization of ethanol‐inducible promoters of Zymomonas mobilis based on omics data and dual reporter–gene system
journal, December 2019

Yang, Yongfu; Rong, Ziyue; Song, Haoyue
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DOI: 10.1002/bab.1838

Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production
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Utilization of both C5 and C6 sugars to produce biofuels and bioproducts is a key goal for the development of integrated lignocellulosic biorefineries. Previously we found that although engineered Zymomonas mobilis 2032 was able to ferment glucose to ethanol when fermenting highly concentrated hydrolyzates such as 9% glucan-loading AFEX-pretreated corn stover hydrolyzate (9% ACSH), xylose conversion after glucose depletion was greatly impaired. We hypothesized that impaired xylose conversion was caused by lignocellulose-derived inhibitors (LDIs) in hydrolyzates. To investigate the effects of LDIs on the cellular physiology of Z. mobilis during fermentation of hydrolyzates, including impacts on xylose utilization, we generatedmore »« less
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Zymomonas mobilis is an ethanologenic alphaproteobacterium with promise for the industrial conversion of renewable plant biomass into fuels and chemical bioproducts. Limited functional annotation of the Z. mobilis genome is a current barrier to both fundamental studies of Z. mobilis and its development as a synthetic biology chassis. To gain insight, we collected sample-matched multiomics data, including RNA sequencing (RNA-seq), transcription start site (TSS) sequencing (TSS-seq), termination sequencing (term-seq), ribosome profiling, and label-free shotgun proteomic mass spectrometry, across different growth conditions and used these data to improve annotation and assign functional sites in the Z. mobilis genome. Proteomics and ribosomemore »« less
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Title: Advances and prospects in metabolic engineering of Zymomonas mobilis

Abstract

Citation Formats

Production of Acetaldehyde by Zymomonas mobilis journal, January 1987

Genetics and genetic engineering ofZymomonas mobilis journal, January 1993

Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment journal, September 2015

A versatile framework for microbial engineering using synthetic non-coding RNAs journal, April 2014

Modular pathway engineering of Bacillus subtilis for improved N-acetylglucosamine production journal, May 2014

Complete Genome Sequence of the Ethanol Producer Zymomonas mobilis NCIMB 11163 journal, September 2009

Engineering lactic acid bacteria with pyruvate decarboxylase and alcohol dehydrogenase genes for ethanol production from Zymomonas mobilis journal, May 2003

Design and construction of improved new vectors for Zymomonas mobilis recombinants journal, March 2011

Reconstruction of a charge balanced genome-scale metabolic model to study the energy-uncoupled growth of Zymomonas mobilis ZM1 journal, January 2016

Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations journal, January 2009

Zymomonas mobilis: a novel platform for future biorefineries journal, January 2014

Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates journal, January 2013

Consolidated bioprocessing of cellulosic biomass: an update journal, October 2005

Expression of Zymomonas mobilis adhB (encoding alcohol dehydrogenase II) and adhB-lacZ operon fusions in recombinant Z. mobilis. journal, September 1989

Small regulatory non-coding RNAs in bacteria: physiology and mechanistic aspects journal, February 2009

Enhancement of 1,3-propanediol production by expression of pyruvate decarboxylase and aldehyde dehydrogenase from Zymomonas mobilis in the acetolactate-synthase-deficient mutant of Klebsiella pneumoniae journal, August 2014

Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. mobilis Lectotype Strain ATCC 10988 journal, July 2011

Using global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis journal, January 2016

Studies on cell-free metabolism: Ethanol production by extracts of Zymomonas mobilis journal, July 1985

Very high gravity ethanol and fatty acid production of Zymomonas mobilis without amino acid and vitamin journal, March 2016

Sorbitol production by Zymomonas mobilis journal, October 1984

Cellulosic ethanol production by Zymomonas mobilis harboring an endoglucanase gene from Enterobacter cloacae journal, February 2011

Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering. journal, January 1996

Comparison of glucose/xylose co-fermentation by recombinant Zymomonas mobilis under different genetic and environmental conditions journal, March 2012

Protein engineering for metabolic engineering: Current and next-generation tools journal, April 2013

Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis journal, January 2015

Secretive expression of heterologous β-glucosidase in Zymomonas mobilis journal, June 2015

Small RNAs in Bacteria journal, April 2002

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs journal, January 2013

Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis journal, January 1995

Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032 journal, May 2018

Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine journal, May 2005

The Listeria transcriptional landscape from saprophytism to virulence journal, May 2009

pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis journal, January 2014

Draft Genome Sequence of the Flocculating Zymomonas mobilis Strain ZM401 (ATCC 31822) journal, December 2012

Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains journal, December 1992

Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production journal, January 2010

Phenotype MicroArray Profiling of Zymomonas mobilis ZM4 journal, December 2009

The Zymomonas mobilis regulator hfq contributes to tolerance against multiple lignocellulosic pretreatment inhibitors journal, January 2010

Expression of the Escherichia coli pmi gene, encoding phosphomannose-isomerase in Zymomonas mobilis, leads to utilization of mannose as a novel growth substrate, which can be used as a selective marker journal, November 1996

The biology of Zymomonas. journal, January 1977

Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis journal, December 2009

d -Glucose Transport System of Zymomonas mobilis journal, January 1985

Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis journal, January 2012

Small RNAs, 5′ UTR elements and RNA-binding proteins in intracellular bacteria: impact on metabolism and virulence journal, May 2015

Genome-scale modeling and in silico analysis of ethanologenic bacteria Zymomonas mobilis journal, November 2010

Microbial production strategies and applications of lycopene and other terpenoids journal, December 2015

The Replacement of Trp392 by Alanine Influences the Decarboxylase/Carboligase Activity and Stability of Pyruvate Decarboxylase from Zymomonas mobilis journal, December 1995

Elucidation of Zymomonas mobilis physiology and stress responses by quantitative proteomics and transcriptomics journal, May 2014

Global transcription machinery engineering: A new approach for improving cellular phenotype journal, May 2007

Modularization of genetic elements promotes synthetic metabolic engineering journal, November 2015

Production of beta-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora. journal, January 1991

Using the CRISPR/Cas9 system to eliminate native plasmids of Zymomonas mobilis ZM4 journal, November 2016

Regulatory RNAs in Bacteria journal, February 2009

Thermostable Variants ofZymomonas mobilisAlcohol Dehydrogenase Obtained Using PCR-Mediated Random Mutagenesis journal, February 1998

Expression of an L-alanine dehydrogenase gene in Zymomonas mobilis and excretion of L-alanine journal, May 1991

Riboswitch regulates RNA journal, August 2014

Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli journal, June 2015

Sucrose utilization by Zymomonas mobilis: formation of a levan journal, March 1966

Construction of expression vectors for the gram-negative bacterium Zymomonas mobilis journal, September 1990

Spanning high-dimensional expression space using ribosome-binding site combinatorics journal, March 2013

Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. pomaceae Lectotype Strain ATCC 29192 journal, July 2011

Tuning genetic control through promoter engineering journal, August 2005

Preparative, in Vitro Biocatalysis of Triketide Lactone Chiral Building Blocks journal, September 2012

Kinetic modelling of the Zymomonas mobilis Entner-Doudoroff pathway: insights into control and functionality journal, October 2013

Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates journal, January 2013

Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis journal, February 2005

irrE, an Exogenous Gene from Deinococcus radiodurans, improves the Growth of and Ethanol Production by a Zymomonas mobilis Strain under Ethanol and Acid Stresses journal, July 2010

Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control journal, July 2010

Coordination of metabolic pathways: Enhanced carbon conservation in 1,3-propanediol production by coupling with optically pure lactate biosynthesis journal, May 2017

Effects of substitution of aspartate-440 and tryptophan-487 in the thiamin diphosphate binding region of pyruvate decarboxylase from Zymomonas mobilis journal, January 1992

Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4 journal, July 2011

Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis journal, March 2010

CRISPR — a widespread system that provides acquired resistance against phages in bacteria and archaea journal, March 2008

DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4 journal, October 2010

The application of microfluidic-based technologies in the cycle of metabolic engineering journal, September 2016

Creating small transcription activating RNAs journal, February 2015

Production of Acetaldehyde by Zymomonas mobilis
journal, January 1987

Genetics and genetic engineering ofZymomonas mobilis
journal, January 1993

Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment
journal, September 2015

A versatile framework for microbial engineering using synthetic non-coding RNAs
journal, April 2014

Modular pathway engineering of Bacillus subtilis for improved N-acetylglucosamine production
journal, May 2014

Complete Genome Sequence of the Ethanol Producer Zymomonas mobilis NCIMB 11163
journal, September 2009

Engineering lactic acid bacteria with pyruvate decarboxylase and alcohol dehydrogenase genes for ethanol production from Zymomonas mobilis
journal, May 2003

Design and construction of improved new vectors for Zymomonas mobilis recombinants
journal, March 2011

Reconstruction of a charge balanced genome-scale metabolic model to study the energy-uncoupled growth of Zymomonas mobilis ZM1
journal, January 2016

Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations
journal, January 2009

Zymomonas mobilis: a novel platform for future biorefineries
journal, January 2014

Inhibition of growth of Zymomonas mobilis by model compounds found in lignocellulosic hydrolysates
journal, January 2013

Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005

Expression of Zymomonas mobilis adhB (encoding alcohol dehydrogenase II) and adhB-lacZ operon fusions in recombinant Z. mobilis.
journal, September 1989

Small regulatory non-coding RNAs in bacteria: physiology and mechanistic aspects
journal, February 2009

Enhancement of 1,3-propanediol production by expression of pyruvate decarboxylase and aldehyde dehydrogenase from Zymomonas mobilis in the acetolactate-synthase-deficient mutant of Klebsiella pneumoniae
journal, August 2014

Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. mobilis Lectotype Strain ATCC 10988
journal, July 2011

Using global transcription machinery engineering (gTME) to improve ethanol tolerance of Zymomonas mobilis
journal, January 2016

Studies on cell-free metabolism: Ethanol production by extracts of Zymomonas mobilis
journal, July 1985

Very high gravity ethanol and fatty acid production of Zymomonas mobilis without amino acid and vitamin
journal, March 2016

Sorbitol production by Zymomonas mobilis
journal, October 1984

Cellulosic ethanol production by Zymomonas mobilis harboring an endoglucanase gene from Enterobacter cloacae
journal, February 2011

Development of an arabinose-fermenting Zymomonas mobilis strain by metabolic pathway engineering.
journal, January 1996

Comparison of glucose/xylose co-fermentation by recombinant Zymomonas mobilis under different genetic and environmental conditions
journal, March 2012

Protein engineering for metabolic engineering: Current and next-generation tools
journal, April 2013

Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis
journal, January 2015

Secretive expression of heterologous β-glucosidase in Zymomonas mobilis
journal, June 2015

Small RNAs in Bacteria
journal, April 2002

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs
journal, January 2013

Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995

Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032
journal, May 2018

Metabolic engineering of Escherichia coli for industrial production of glucosamine and N-acetylglucosamine
journal, May 2005

The Listeria transcriptional landscape from saprophytism to virulence
journal, May 2009

pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis
journal, January 2014

Draft Genome Sequence of the Flocculating Zymomonas mobilis Strain ZM401 (ATCC 31822)
journal, December 2012

Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains
journal, December 1992

Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production
journal, January 2010

Phenotype MicroArray Profiling of Zymomonas mobilis ZM4
journal, December 2009

The Zymomonas mobilis regulator hfq contributes to tolerance against multiple lignocellulosic pretreatment inhibitors
journal, January 2010

Expression of the Escherichia coli pmi gene, encoding phosphomannose-isomerase in Zymomonas mobilis, leads to utilization of mannose as a novel growth substrate, which can be used as a selective marker
journal, November 1996

The biology of Zymomonas.
journal, January 1977

Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis
journal, December 2009

d -Glucose Transport System of Zymomonas mobilis
journal, January 1985

Cellulosic Ethanol Production by Recombinant Cellulolytic Bacteria Harbouring pdc and adh II Genes of Zymomonas mobilis
journal, January 2012

Small RNAs, 5′ UTR elements and RNA-binding proteins in intracellular bacteria: impact on metabolism and virulence
journal, May 2015

Genome-scale modeling and in silico analysis of ethanologenic bacteria Zymomonas mobilis
journal, November 2010

Microbial production strategies and applications of lycopene and other terpenoids
journal, December 2015

The Replacement of Trp392 by Alanine Influences the Decarboxylase/Carboligase Activity and Stability of Pyruvate Decarboxylase from Zymomonas mobilis
journal, December 1995

Elucidation of Zymomonas mobilis physiology and stress responses by quantitative proteomics and transcriptomics
journal, May 2014

Global transcription machinery engineering: A new approach for improving cellular phenotype
journal, May 2007

Modularization of genetic elements promotes synthetic metabolic engineering
journal, November 2015

Production of beta-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora.
journal, January 1991

Using the CRISPR/Cas9 system to eliminate native plasmids of Zymomonas mobilis ZM4
journal, November 2016

Regulatory RNAs in Bacteria
journal, February 2009

Thermostable Variants ofZymomonas mobilisAlcohol Dehydrogenase Obtained Using PCR-Mediated Random Mutagenesis
journal, February 1998

Expression of an L-alanine dehydrogenase gene in Zymomonas mobilis and excretion of L-alanine
journal, May 1991

Riboswitch regulates RNA
journal, August 2014

Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
journal, June 2015

Sucrose utilization by Zymomonas mobilis: formation of a levan
journal, March 1966

Construction of expression vectors for the gram-negative bacterium Zymomonas mobilis
journal, September 1990

Spanning high-dimensional expression space using ribosome-binding site combinatorics
journal, March 2013

Genome Sequence of the Ethanol-Producing Zymomonas mobilis subsp. pomaceae Lectotype Strain ATCC 29192
journal, July 2011

Tuning genetic control through promoter engineering
journal, August 2005

Preparative, in Vitro Biocatalysis of Triketide Lactone Chiral Building Blocks
journal, September 2012

Kinetic modelling of the Zymomonas mobilis Entner-Doudoroff pathway: insights into control and functionality
journal, October 2013

Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates
journal, January 2013

Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis
journal, February 2005

irrE, an Exogenous Gene from Deinococcus radiodurans, improves the Growth of and Ethanol Production by a Zymomonas mobilis Strain under Ethanol and Acid Stresses
journal, July 2010

Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control
journal, July 2010

Coordination of metabolic pathways: Enhanced carbon conservation in 1,3-propanediol production by coupling with optically pure lactate biosynthesis
journal, May 2017

Effects of substitution of aspartate-440 and tryptophan-487 in the thiamin diphosphate binding region of pyruvate decarboxylase from Zymomonas mobilis
journal, January 1992

Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4
journal, July 2011

Enhanced production of ethanol from glycerol by engineered Hansenula polymorpha expressing pyruvate decarboxylase and aldehyde dehydrogenase genes from Zymomonas mobilis
journal, March 2010

CRISPR — a widespread system that provides acquired resistance against phages in bacteria and archaea
journal, March 2008

DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4
journal, October 2010

The application of microfluidic-based technologies in the cycle of metabolic engineering
journal, September 2016

Creating small transcription activating RNAs
journal, February 2015

3′-UTRs as a source of regulatory RNAs in bacteria: 3′-UTRs as a source of regulatory RNAs in bacteria
journal, September 2012

Zymomonas mobilis as a model system for production of biofuels and biochemicals
journal, September 2016

GLYCOLYTIC ENZYMES IN ZYMOMONAS MOBILIS
journal, July 1962