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Title: Variation in energy sorghum hybrid TX08001 biomass composition and lignin chemistry during development under irrigated and non-irrigated field conditions

Abstract

This study was conducted to document the extent and basis of compositional variation of shoot biomass of the energy Sorghum bicolor hybrid TX08001 during development under field conditions. TX08001 is capable of accumulating ~40 Mg/ha of dry biomass under good growing conditions and this genotype allocates ~80% of its shoot biomass to stems. After 150 days of growth TX08001 stems had a fresh/dry weight ratio of ~3:1 and soluble biomass accounted for ~30% of stem biomass. A panel of diverse energy sorghum genotypes varied ~6-fold in the ratio of stem structural to soluble biomass after 150 days of growth. Near-infrared spectroscopic analysis (NIRS) showed that TX08001 leaves accumulated higher levels of protein, water extractives and ash compared to stems, which have higher sugar, cellulose, and lignin contents. TX08001 stem sucrose content varied during development, whereas the composition of TX08001 stem cell walls, which consisted of ~45–49% cellulose, ~27–30% xylan, and ~15–18% lignin, remained constant after 90 days post emergence until the end of the growing season (180 days). TX08001 and Della stem syringyl (S)/guaiacyl (G) (0.53–0.58) and ferulic acid (FA)/para-coumaric acid (pCA) ratios were similar whereas ratios of pCA/(S+G) differed between these genotypes. Additionally, an analysis of irrigated versus non-irrigatedmore » TX08001 revealed that non-irrigated hybrids exhibited a 50% reduction in total cell wall biomass, an ~2-fold increase in stem sugars, and an ~25% increase in water extractives relative to irrigated hybrids. This study provides a baseline of information to help guide further optimization of energy sorghum composition for various end-uses.« less

Authors:
ORCiD logo [1];  [2];  [2];  [3];  [4];  [5];  [5];  [6];  [1]
  1. Texas A & M Univ., College Station, TX (United States). Department of Biochemistry and Biophysics; Department of Energy Great Lakes Bioenergy Research Center (United States)
  2. Texas A & M Univ., College Station, TX (United States). Department of Biochemistry and Biophysics
  3. Oregon State Univ., Corvallis, OR (United States). Department of Crop and Soil Science
  4. Department of Energy Great Lakes Bioenergy Research Center (United States); Univ. of Wisconsin, Madison, WI (United States). Department of Biochemistry, the Wisconsin Energy Institute
  5. Department of Energy Great Lakes Bioenergy Research Center (United States); Univ. of Wisconsin, Madison, WI (United States). Department of Biochemistry, the Wisconsin Energy Institute
  6. Texas A & M Univ., College Station, TX (United States). Department of Soil and Crop Sciences
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1459514
Grant/Contract Number:  
SC0018409; SC0006930
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 13; Journal Issue: 4; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Sorghum; Lignin; Leaves; Near-infrared spectroscopy; Internodes; Plant cell walls; Crops; Carbohydrates

Citation Formats

McKinley, Brian A., Olson, Sara N., Ritter, Kimberley B., Herb, Dustin W., Karlen, Steven D., Lu, Fachuang, Ralph, John, Rooney, William L., and Mullet, John E. Variation in energy sorghum hybrid TX08001 biomass composition and lignin chemistry during development under irrigated and non-irrigated field conditions. United States: N. p., 2018. Web. doi:10.1371/journal.pone.0195863.
McKinley, Brian A., Olson, Sara N., Ritter, Kimberley B., Herb, Dustin W., Karlen, Steven D., Lu, Fachuang, Ralph, John, Rooney, William L., & Mullet, John E. Variation in energy sorghum hybrid TX08001 biomass composition and lignin chemistry during development under irrigated and non-irrigated field conditions. United States. doi:10.1371/journal.pone.0195863.
McKinley, Brian A., Olson, Sara N., Ritter, Kimberley B., Herb, Dustin W., Karlen, Steven D., Lu, Fachuang, Ralph, John, Rooney, William L., and Mullet, John E. Mon . "Variation in energy sorghum hybrid TX08001 biomass composition and lignin chemistry during development under irrigated and non-irrigated field conditions". United States. doi:10.1371/journal.pone.0195863. https://www.osti.gov/servlets/purl/1459514.
@article{osti_1459514,
title = {Variation in energy sorghum hybrid TX08001 biomass composition and lignin chemistry during development under irrigated and non-irrigated field conditions},
author = {McKinley, Brian A. and Olson, Sara N. and Ritter, Kimberley B. and Herb, Dustin W. and Karlen, Steven D. and Lu, Fachuang and Ralph, John and Rooney, William L. and Mullet, John E.},
abstractNote = {This study was conducted to document the extent and basis of compositional variation of shoot biomass of the energy Sorghum bicolor hybrid TX08001 during development under field conditions. TX08001 is capable of accumulating ~40 Mg/ha of dry biomass under good growing conditions and this genotype allocates ~80% of its shoot biomass to stems. After 150 days of growth TX08001 stems had a fresh/dry weight ratio of ~3:1 and soluble biomass accounted for ~30% of stem biomass. A panel of diverse energy sorghum genotypes varied ~6-fold in the ratio of stem structural to soluble biomass after 150 days of growth. Near-infrared spectroscopic analysis (NIRS) showed that TX08001 leaves accumulated higher levels of protein, water extractives and ash compared to stems, which have higher sugar, cellulose, and lignin contents. TX08001 stem sucrose content varied during development, whereas the composition of TX08001 stem cell walls, which consisted of ~45–49% cellulose, ~27–30% xylan, and ~15–18% lignin, remained constant after 90 days post emergence until the end of the growing season (180 days). TX08001 and Della stem syringyl (S)/guaiacyl (G) (0.53–0.58) and ferulic acid (FA)/para-coumaric acid (pCA) ratios were similar whereas ratios of pCA/(S+G) differed between these genotypes. Additionally, an analysis of irrigated versus non-irrigated TX08001 revealed that non-irrigated hybrids exhibited a 50% reduction in total cell wall biomass, an ~2-fold increase in stem sugars, and an ~25% increase in water extractives relative to irrigated hybrids. This study provides a baseline of information to help guide further optimization of energy sorghum composition for various end-uses.},
doi = {10.1371/journal.pone.0195863},
journal = {PLoS ONE},
number = 4,
volume = 13,
place = {United States},
year = {2018},
month = {4}
}

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