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Title: In the grass species Brachypodium distachyon, the production of mixed-linkage (1,3;1,4)-β-glucan (MLG) occurs in the Golgi apparatus

Abstract

Summary Mixed‐linkage (1,3;1,4)‐β‐glucan ( MLG ) is a glucose polymer with beneficial effects on human health and high potential for the agricultural industry. MLG is present predominantly in the cell wall of grasses and is synthesized by cellulose synthase‐like F or H families of proteins, with CSLF 6 being the best‐characterized MLG synthase. Although the function of this enzyme in MLG production has been established, the site of MLG synthesis in the cell is debated. It has been proposed that MLG is synthesized at the plasma membrane, as occurs for cellulose and callose; in contrast, it has also been proposed that MLG is synthesized in the Golgi apparatus, as occurs for other matrix polysaccharides of the cell wall. Testing these conflicting possibilities is fundamentally important in the general understanding of the biosynthesis of the plant cell wall. Using immuno‐localization analyses with MLG ‐specific antibody in Brachypodium and in barley, we found MLG present in the Golgi, in post‐Golgi structures and in the cell wall. Accordingly, analyses of a functional fluorescent protein fusion of CSLF 6 stably expressed in Brachypodium demonstrated that the enzyme is localized in the Golgi. We also established that overproduction of MLG causes developmental and growth defectsmore » in Brachypodium as also occur in barley. Our results indicated that MLG production occurs in the Golgi similarly to other cell wall matrix polysaccharides, and supports the broadly applicable model in grasses that tight mechanisms control optimal MLG accumulation in the cell wall during development and growth. This work addresses the fundamental question of where mixed linkage (1,3;1,4)‐β‐glucan (MLG) is synthesized in plant cells. By analyzing the subcellular localization of MLG and MLG synthase in an endogenous system, we demonstrated that MLG synthesis occurs at the Golgi in Brachypodium and barley. A growth inhibition due to overproduced MLG in Brachypodium supports the general applicability of the model that a tight control of the cell wall polysaccharides accumulation is needed to maintain growth homeostasis during development.« less

Authors:
ORCiD logo [1];  [1];  [2];  [3];  [4];  [5]
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  1. Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center
  2. Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center; Michigan State Univ., East Lansing, MI (United States). Dept. of Plant Biology
  3. Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center; Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry & Molecular Biology; Michigan State Univ., East Lansing, MI (United States). Dept. of Plant Biology
  4. Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center; Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry & Molecular Biology
  5. Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center; Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Michigan State Univ., East Lansing, MI (United States). Dept. of Plant Biology
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States). Great Lakes Bioenergy Research Center
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1459435
Alternate Identifier(s):
OSTI ID: 1424806
Grant/Contract Number:  
SC0018409; FC02-07ER64494; FG02-91ER20021; DE‐FG02‐91ER20021
Resource Type:
Accepted Manuscript
Journal Name:
The Plant Journal
Additional Journal Information:
Journal Volume: 93; Journal Issue: 6; Journal ID: ISSN 0960-7412
Publisher:
Society for Experimental Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CSLF6; mixed-linkage glucan; Golgi; Brachypodium distachyon; Brefeldin A

Citation Formats

Kim, Sang-Jin, Zemelis-Durfee, Starla, Jensen, Jacob Krüger, Wilkerson, Curtis G., Keegstra, Kenneth, and Brandizzi, Federica. In the grass species Brachypodium distachyon, the production of mixed-linkage (1,3;1,4)-β-glucan (MLG) occurs in the Golgi apparatus. United States: N. p., 2018. Web. doi:10.1111/tpj.13830.
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Kim, Sang-Jin, Zemelis-Durfee, Starla, Jensen, Jacob Krüger, Wilkerson, Curtis G., Keegstra, Kenneth, & Brandizzi, Federica. In the grass species Brachypodium distachyon, the production of mixed-linkage (1,3;1,4)-β-glucan (MLG) occurs in the Golgi apparatus. United States. https://doi.org/10.1111/tpj.13830
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Kim, Sang-Jin, Zemelis-Durfee, Starla, Jensen, Jacob Krüger, Wilkerson, Curtis G., Keegstra, Kenneth, and Brandizzi, Federica. Sat . "In the grass species Brachypodium distachyon, the production of mixed-linkage (1,3;1,4)-β-glucan (MLG) occurs in the Golgi apparatus". United States. https://doi.org/10.1111/tpj.13830. https://www.osti.gov/servlets/purl/1459435.
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@article{osti_1459435,
title = {In the grass species Brachypodium distachyon, the production of mixed-linkage (1,3;1,4)-β-glucan (MLG) occurs in the Golgi apparatus},
author = {Kim, Sang-Jin and Zemelis-Durfee, Starla and Jensen, Jacob Krüger and Wilkerson, Curtis G. and Keegstra, Kenneth and Brandizzi, Federica},
abstractNote = {Summary Mixed‐linkage (1,3;1,4)‐β‐glucan ( MLG ) is a glucose polymer with beneficial effects on human health and high potential for the agricultural industry. MLG is present predominantly in the cell wall of grasses and is synthesized by cellulose synthase‐like F or H families of proteins, with CSLF 6 being the best‐characterized MLG synthase. Although the function of this enzyme in MLG production has been established, the site of MLG synthesis in the cell is debated. It has been proposed that MLG is synthesized at the plasma membrane, as occurs for cellulose and callose; in contrast, it has also been proposed that MLG is synthesized in the Golgi apparatus, as occurs for other matrix polysaccharides of the cell wall. Testing these conflicting possibilities is fundamentally important in the general understanding of the biosynthesis of the plant cell wall. Using immuno‐localization analyses with MLG ‐specific antibody in Brachypodium and in barley, we found MLG present in the Golgi, in post‐Golgi structures and in the cell wall. Accordingly, analyses of a functional fluorescent protein fusion of CSLF 6 stably expressed in Brachypodium demonstrated that the enzyme is localized in the Golgi. We also established that overproduction of MLG causes developmental and growth defects in Brachypodium as also occur in barley. Our results indicated that MLG production occurs in the Golgi similarly to other cell wall matrix polysaccharides, and supports the broadly applicable model in grasses that tight mechanisms control optimal MLG accumulation in the cell wall during development and growth. This work addresses the fundamental question of where mixed linkage (1,3;1,4)‐β‐glucan (MLG) is synthesized in plant cells. By analyzing the subcellular localization of MLG and MLG synthase in an endogenous system, we demonstrated that MLG synthesis occurs at the Golgi in Brachypodium and barley. A growth inhibition due to overproduced MLG in Brachypodium supports the general applicability of the model that a tight control of the cell wall polysaccharides accumulation is needed to maintain growth homeostasis during development.},
doi = {10.1111/tpj.13830},
journal = {The Plant Journal},
number = 6,
volume = 93,
place = {United States},
year = {Sat Jan 27 00:00:00 EST 2018},
month = {Sat Jan 27 00:00:00 EST 2018}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1111/tpj.13830
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Cited by: 15 works
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Figures / Tables:

Table 1 Table 1: Number of gold particles (MLG) in the designated components in the cell
All figures and tables (13 total)
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    Works referencing / citing this record:

    Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling in Arabidopsis
    journal, December 2019

    Post-Golgi Trafficking and Transport of Cell Wall Components
    journal, December 2018

    • Sinclair, Rosalie; Rosquete, Michel Ruiz; Drakakaki, Georgia
    • Frontiers in Plant Science, Vol. 9
    • DOI: 10.3389/fpls.2018.01784
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      Figures / Tables found in this record:

      Table 1 (p. 26)table
      Table 2 (p. 27)table
      Figure 1 (p. 31)figure
      Figure 2 (p. 32)figure
      Figure 3 (p. 33)figure
      Figure 4 (p. 34)figure
      Figure 5 (p. 35)figure
      Table 1 (p. 36)table
      Figure S1 (p. 37)figure
      Figure S2 (p. 38)figure
      Figure S3 (p. 40)figure
      Table 2 (p. 41)table
      Table S1 (p. 42)table
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