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Title: Microbial metal resistance and metabolism across dynamic landscapes: high-throughput environmental microbiology

Abstract

Multidimensional gradients of inorganic compounds influence microbial activity in diverse pristine and anthropogenically perturbed environments. Here, we suggest that high-throughput cultivation and genetics can be systematically applied to generate quantitative models linking gene function, microbial community activity, and geochemical parameters. Metal resistance determinants represent a uniquely universal set of parameters around which to study and evaluate microbial fitness because they represent a record of the environment in which all microbial life evolved. By cultivating microbial isolates and enrichments in laboratory gradients of inorganic ions, we can generate quantitative predictions of limits on microbial range in the environment, obtain more accurate gene annotations, and identify useful strategies for predicting and engineering the trajectory of natural ecosystems.

Authors:
; ; ORCiD logo
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1459229
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
F1000Research
Additional Journal Information:
Journal Name: F1000Research Journal Volume: 6; Journal ID: ISSN 2046-1402
Publisher:
F1000 Research, Ltd.
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Carlson, Hans, Deutschbauer, Adam, and Coates, John. Microbial metal resistance and metabolism across dynamic landscapes: high-throughput environmental microbiology. United Kingdom: N. p., 2017. Web. doi:10.12688/f1000research.10986.1.
Carlson, Hans, Deutschbauer, Adam, & Coates, John. Microbial metal resistance and metabolism across dynamic landscapes: high-throughput environmental microbiology. United Kingdom. doi:10.12688/f1000research.10986.1.
Carlson, Hans, Deutschbauer, Adam, and Coates, John. Thu . "Microbial metal resistance and metabolism across dynamic landscapes: high-throughput environmental microbiology". United Kingdom. doi:10.12688/f1000research.10986.1.
@article{osti_1459229,
title = {Microbial metal resistance and metabolism across dynamic landscapes: high-throughput environmental microbiology},
author = {Carlson, Hans and Deutschbauer, Adam and Coates, John},
abstractNote = {Multidimensional gradients of inorganic compounds influence microbial activity in diverse pristine and anthropogenically perturbed environments. Here, we suggest that high-throughput cultivation and genetics can be systematically applied to generate quantitative models linking gene function, microbial community activity, and geochemical parameters. Metal resistance determinants represent a uniquely universal set of parameters around which to study and evaluate microbial fitness because they represent a record of the environment in which all microbial life evolved. By cultivating microbial isolates and enrichments in laboratory gradients of inorganic ions, we can generate quantitative predictions of limits on microbial range in the environment, obtain more accurate gene annotations, and identify useful strategies for predicting and engineering the trajectory of natural ecosystems.},
doi = {10.12688/f1000research.10986.1},
journal = {F1000Research},
number = ,
volume = 6,
place = {United Kingdom},
year = {2017},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.12688/f1000research.10986.1

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Works referenced in this record:

Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons
journal, May 2015

  • Wetmore, Kelly M.; Price, Morgan N.; Waters, Robert J.
  • mBio, Vol. 6, Issue 3, Article No. e00306-15
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