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Title: CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes

Abstract

In recent years CRISPR-Cas technologies have revolutionized microbial engineering approaches. Genome editing and non-editing applications of various CRISPR-Cas systems have expanded the throughput and scale of engineering efforts, as well as opened up new avenues for manipulating genomes of non-model organisms. As we expand the range of organisms used for biotechnological applications, we need to develop better, more versatile tools for manipulation of these systems. Here we summarize the current advances in microbial gene editing using CRISPR-Cas based tools, and highlight state-of-the-art methods for high-throughput, efficient genome-scale engineering in model organisms Escherichia coli and Saccharomyces cerevisiae. We also review non-editing CRISPR-Cas applications available for gene expression manipulation, epigenetic remodeling, RNA editing, labeling and synthetic gene circuit design. Finally, we point out the areas of research that need further development in order to expand the range of applications and increase the utility of these new methods.

Authors:
 [1];  [1];  [2];  [1]
  1. Univ. of Colorado, Boulder, CO (United States)
  2. Univ. of Colorado, Boulder, CO (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1458905
Alternate Identifier(s):
OSTI ID: 1557854
Report Number(s):
NREL/JA-2700-71862
Journal ID: ISSN 1860-6768
Grant/Contract Number:  
AC36-08GO28308; SC0018368
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology Journal
Additional Journal Information:
Journal Volume: 13; Journal Issue: 9; Journal ID: ISSN 1860-6768
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CRISPR editing; CRISPR gene regulation; CRISPR tools for microbes; CRISPRa; CRISPRi

Citation Formats

Tarasava, Katia, Oh, Eun Joong, Eckert, Carrie A., and Gill, Ryan T. CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes. United States: N. p., 2018. Web. doi:10.1002/biot.201700586.
Tarasava, Katia, Oh, Eun Joong, Eckert, Carrie A., & Gill, Ryan T. CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes. United States. doi:10.1002/biot.201700586.
Tarasava, Katia, Oh, Eun Joong, Eckert, Carrie A., and Gill, Ryan T. Tue . "CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes". United States. doi:10.1002/biot.201700586. https://www.osti.gov/servlets/purl/1458905.
@article{osti_1458905,
title = {CRISPR-Enabled Tools for Engineering Microbial Genomes and Phenotypes},
author = {Tarasava, Katia and Oh, Eun Joong and Eckert, Carrie A. and Gill, Ryan T.},
abstractNote = {In recent years CRISPR-Cas technologies have revolutionized microbial engineering approaches. Genome editing and non-editing applications of various CRISPR-Cas systems have expanded the throughput and scale of engineering efforts, as well as opened up new avenues for manipulating genomes of non-model organisms. As we expand the range of organisms used for biotechnological applications, we need to develop better, more versatile tools for manipulation of these systems. Here we summarize the current advances in microbial gene editing using CRISPR-Cas based tools, and highlight state-of-the-art methods for high-throughput, efficient genome-scale engineering in model organisms Escherichia coli and Saccharomyces cerevisiae. We also review non-editing CRISPR-Cas applications available for gene expression manipulation, epigenetic remodeling, RNA editing, labeling and synthetic gene circuit design. Finally, we point out the areas of research that need further development in order to expand the range of applications and increase the utility of these new methods.},
doi = {10.1002/biot.201700586},
journal = {Biotechnology Journal},
number = 9,
volume = 13,
place = {United States},
year = {2018},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
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Cited by: 8 works
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Figures / Tables:

Table 1. Table 1.: CRISPR-enabled genome editing in non-model microbes.

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    Works referencing / citing this record:

    CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals
    journal, July 2019

    • Mitsui, Ryosuke; Yamada, Ryosuke; Ogino, Hiroyasu
    • World Journal of Microbiology and Biotechnology, Vol. 35, Issue 7
    • DOI: 10.1007/s11274-019-2688-8

    CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals
    journal, July 2019

    • Mitsui, Ryosuke; Yamada, Ryosuke; Ogino, Hiroyasu
    • World Journal of Microbiology and Biotechnology, Vol. 35, Issue 7
    • DOI: 10.1007/s11274-019-2688-8

      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.