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Title: A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion

Abstract

Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Here, structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.

Authors:
 [1]; ORCiD logo [2];  [3]; ORCiD logo [4];  [5]; ORCiD logo [6];  [1]; ORCiD logo [7]; ORCiD logo [6]; ORCiD logo [8];  [4]; ORCiD logo [6];  [9]; ORCiD logo [1]
  1. Univ. of Portsmouth, Portsmouth (United Kingdom)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States); Montana State Univ., Bozeman, MT (United States)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Campinas, Sao Paulo (Brazil)
  4. Univ. of California at Los Angeles, Los Angeles, CA (United States)
  5. National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of California at Los Angeles, Los Angeles, CA (United States)
  6. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  7. Univ. of Campinas, Sao Paulo (Brazil)
  8. Univ. of Georgia, Athens, GA (United States)
  9. Montana State Univ., Bozeman, MT (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1458818
Report Number(s):
NREL/JA-5100-71206
Journal ID: ISSN 2041-1723
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; enzyme discovery; protein family; enzyme characterization; lignin conversion

Citation Formats

Mallinson, Sam J. B., Machovina, Melodie M., Silveira, Rodrigo L., Garcia-Borras, Marc, Gallup, Nathan, Johnson, Christopher W., Allen, Mark D., Skaf, Munir S., Crowley, Michael F., Neidle, Ellen L., Houk, Kendall N., Beckham, Gregg T., DuBois, Jennifer L., and McGeehan, John E. A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion. United States: N. p., 2018. Web. https://doi.org/10.1038/s41467-018-04878-2.
Mallinson, Sam J. B., Machovina, Melodie M., Silveira, Rodrigo L., Garcia-Borras, Marc, Gallup, Nathan, Johnson, Christopher W., Allen, Mark D., Skaf, Munir S., Crowley, Michael F., Neidle, Ellen L., Houk, Kendall N., Beckham, Gregg T., DuBois, Jennifer L., & McGeehan, John E. A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion. United States. https://doi.org/10.1038/s41467-018-04878-2
Mallinson, Sam J. B., Machovina, Melodie M., Silveira, Rodrigo L., Garcia-Borras, Marc, Gallup, Nathan, Johnson, Christopher W., Allen, Mark D., Skaf, Munir S., Crowley, Michael F., Neidle, Ellen L., Houk, Kendall N., Beckham, Gregg T., DuBois, Jennifer L., and McGeehan, John E. Wed . "A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion". United States. https://doi.org/10.1038/s41467-018-04878-2. https://www.osti.gov/servlets/purl/1458818.
@article{osti_1458818,
title = {A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion},
author = {Mallinson, Sam J. B. and Machovina, Melodie M. and Silveira, Rodrigo L. and Garcia-Borras, Marc and Gallup, Nathan and Johnson, Christopher W. and Allen, Mark D. and Skaf, Munir S. and Crowley, Michael F. and Neidle, Ellen L. and Houk, Kendall N. and Beckham, Gregg T. and DuBois, Jennifer L. and McGeehan, John E.},
abstractNote = {Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Here, structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.},
doi = {10.1038/s41467-018-04878-2},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {6}
}

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