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Title: Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels

Achieving low cost and high efficiency lignocellulose deconstruction is a critical step towards widespread adoption of lignocellulosic biofuels. Certain ionic liquid (IL)-based pretreatment processes effectively reduce recalcitrance of lignocellulose to enzymatic degradation but require either costly separations following pretreatment or novel IL compatible processes to mitigate downstream toxicity. Here we demonstrate at benchtop and pilot bioreactor scales a separation-free, intensified process for IL pretreatment, saccharification, and fermentation of sorghum biomass to produce the sesquiterpene bisabolene, a precursor to the renewable diesel and jet fuel bisabolane. The deconstruction process employs the IL cholinium lysinate ([Ch][Lys]), followed by enzymatic saccharification with the commercial enzyme cocktails Cellic CTec2 and HTec2. Glucose yields above 80% and xylose yields above 60% are observed at all scales tested. Unfiltered hydrolysate is fermented directly by Rhodosporidium toruloides – with glucose, xylose, acetate and lactate fully consumed during fermentation at all scales tested. Bisabolene titers improved with scale from 1.3 g L –1 in 30 mL shake flasks to 2.2 g L –1 in 20 L fermentation. The combined process enables conversion of saccharified IL-pretreated biomass directly to advanced biofuels with no separations or washing, minimal additions to facilitate fermentation, no loss of performance due to IL toxicity,more » and simplified fuel recovery via phase separation. In conclusion, this study is the first to demonstrate a separation-free IL based process for conversion of biomass to an advanced biofuel and is the first to demonstrate full consumption of glucose, xylose, acetate, and lactic acid in the presence of [Ch][Lys].« less
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [3] ;  [4] ;  [1] ; ORCiD logo [1] ; ORCiD logo [1] ;  [1] ;  [1] ; ORCiD logo [4] ; ORCiD logo [3] ;  [1] ;  [5] ; ORCiD logo [4]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Joint BioEnergy Institute, Emeryville, CA (United States)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute, Emeryville, CA (United States)
  4. Joint BioEnergy Institute, Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  5. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Grant/Contract Number:
AC02-05CH11231; AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Green Chemistry
Additional Journal Information:
Journal Volume: 20; Journal Issue: 12; Journal ID: ISSN 1463-9262
Publisher:
Royal Society of Chemistry
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
OSTI Identifier:
1458771

Sundstrom, Eric, Yaegashi, Junko, Yan, Jipeng, Masson, Fabrice, Papa, Gabriella, Rodriguez, Alberto, Mirsiaghi, Mona, Liang, Ling, He, Qian, Tanjore, Deepti, Pray, Todd R., Singh, Seema, Simmons, Blake, Sun, Ning, Magnuson, Jon, and Gladden, John. Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels. United States: N. p., Web. doi:10.1039/c8gc00518d.
Sundstrom, Eric, Yaegashi, Junko, Yan, Jipeng, Masson, Fabrice, Papa, Gabriella, Rodriguez, Alberto, Mirsiaghi, Mona, Liang, Ling, He, Qian, Tanjore, Deepti, Pray, Todd R., Singh, Seema, Simmons, Blake, Sun, Ning, Magnuson, Jon, & Gladden, John. Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels. United States. doi:10.1039/c8gc00518d.
Sundstrom, Eric, Yaegashi, Junko, Yan, Jipeng, Masson, Fabrice, Papa, Gabriella, Rodriguez, Alberto, Mirsiaghi, Mona, Liang, Ling, He, Qian, Tanjore, Deepti, Pray, Todd R., Singh, Seema, Simmons, Blake, Sun, Ning, Magnuson, Jon, and Gladden, John. 2018. "Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels". United States. doi:10.1039/c8gc00518d. https://www.osti.gov/servlets/purl/1458771.
@article{osti_1458771,
title = {Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels},
author = {Sundstrom, Eric and Yaegashi, Junko and Yan, Jipeng and Masson, Fabrice and Papa, Gabriella and Rodriguez, Alberto and Mirsiaghi, Mona and Liang, Ling and He, Qian and Tanjore, Deepti and Pray, Todd R. and Singh, Seema and Simmons, Blake and Sun, Ning and Magnuson, Jon and Gladden, John},
abstractNote = {Achieving low cost and high efficiency lignocellulose deconstruction is a critical step towards widespread adoption of lignocellulosic biofuels. Certain ionic liquid (IL)-based pretreatment processes effectively reduce recalcitrance of lignocellulose to enzymatic degradation but require either costly separations following pretreatment or novel IL compatible processes to mitigate downstream toxicity. Here we demonstrate at benchtop and pilot bioreactor scales a separation-free, intensified process for IL pretreatment, saccharification, and fermentation of sorghum biomass to produce the sesquiterpene bisabolene, a precursor to the renewable diesel and jet fuel bisabolane. The deconstruction process employs the IL cholinium lysinate ([Ch][Lys]), followed by enzymatic saccharification with the commercial enzyme cocktails Cellic CTec2 and HTec2. Glucose yields above 80% and xylose yields above 60% are observed at all scales tested. Unfiltered hydrolysate is fermented directly by Rhodosporidium toruloides – with glucose, xylose, acetate and lactate fully consumed during fermentation at all scales tested. Bisabolene titers improved with scale from 1.3 g L–1 in 30 mL shake flasks to 2.2 g L–1 in 20 L fermentation. The combined process enables conversion of saccharified IL-pretreated biomass directly to advanced biofuels with no separations or washing, minimal additions to facilitate fermentation, no loss of performance due to IL toxicity, and simplified fuel recovery via phase separation. In conclusion, this study is the first to demonstrate a separation-free IL based process for conversion of biomass to an advanced biofuel and is the first to demonstrate full consumption of glucose, xylose, acetate, and lactic acid in the presence of [Ch][Lys].},
doi = {10.1039/c8gc00518d},
journal = {Green Chemistry},
number = 12,
volume = 20,
place = {United States},
year = {2018},
month = {6}
}

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