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Title: Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Abstract

Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. In conclusion, we anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2];  [2]; ORCiD logo [3];  [4];  [5];  [6];  [5];  [2];  [2]; ORCiD logo [7];  [8];  [9]; ORCiD logo [8]; ORCiD logo [2];  [10]; ORCiD logo [4];  [11];  [11] more »; ORCiD logo [11];  [11];  [12];  [6]; ORCiD logo [5];  [2]; ORCiD logo [13] « less
  1. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Centre for Ultrafast Imaging, Hamburg (Germany)
  2. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  3. Univ. of Zurich, Zurich (Switzerland)
  4. Univ. of Basel, Basel (Switzerland)
  5. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  6. Univ. of Canterbury, Christchurch (New Zealand)
  7. Dept. of Chemistry and Molecular Biology, Gothenburg (Sweden)
  8. Univ. de Bordeaux, Pessac (France)
  9. Univ. of Copenhagen, Copenhagen (Denmark)
  10. ETH Zurich, Zurich (Switzerland)
  11. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  12. Univ. of Oxford, Oxfordshire (United Kingdom); Diamond Light Source, Oxfordshire (United Kingdom)
  13. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Centre for Ultrafast Imaging, Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1458736
Grant/Contract Number:  
AC02-76SF00515
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Seuring, Carolin, Ayyer, Kartik, Filippaki, Eleftheria, Barthelmess, Miriam, Longchamp, Jean -Nicolas, Ringler, Philippe, Pardini, Tommaso, Wojtas, David H., Coleman, Matthew A., Dorner, Katerina, Fuglerud, Silje, Hammarin, Greger, Habenstein, Birgit, Langkilde, Annette E., Loquet, Antoine, Meents, Alke, Riek, Roland, Stahlberg, Henning, Boutet, Sebastien, Hunter, Mark S., Koglin, Jason, Liang, Mengning, Ginn, Helen M., Millane, Rick P., Frank, Matthias, Barty, Anton, and Chapman, Henry N. Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. United States: N. p., 2018. Web. doi:10.1038/s41467-018-04116-9.
Seuring, Carolin, Ayyer, Kartik, Filippaki, Eleftheria, Barthelmess, Miriam, Longchamp, Jean -Nicolas, Ringler, Philippe, Pardini, Tommaso, Wojtas, David H., Coleman, Matthew A., Dorner, Katerina, Fuglerud, Silje, Hammarin, Greger, Habenstein, Birgit, Langkilde, Annette E., Loquet, Antoine, Meents, Alke, Riek, Roland, Stahlberg, Henning, Boutet, Sebastien, Hunter, Mark S., Koglin, Jason, Liang, Mengning, Ginn, Helen M., Millane, Rick P., Frank, Matthias, Barty, Anton, & Chapman, Henry N. Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. United States. doi:10.1038/s41467-018-04116-9.
Seuring, Carolin, Ayyer, Kartik, Filippaki, Eleftheria, Barthelmess, Miriam, Longchamp, Jean -Nicolas, Ringler, Philippe, Pardini, Tommaso, Wojtas, David H., Coleman, Matthew A., Dorner, Katerina, Fuglerud, Silje, Hammarin, Greger, Habenstein, Birgit, Langkilde, Annette E., Loquet, Antoine, Meents, Alke, Riek, Roland, Stahlberg, Henning, Boutet, Sebastien, Hunter, Mark S., Koglin, Jason, Liang, Mengning, Ginn, Helen M., Millane, Rick P., Frank, Matthias, Barty, Anton, and Chapman, Henry N. Wed . "Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene". United States. doi:10.1038/s41467-018-04116-9. https://www.osti.gov/servlets/purl/1458736.
@article{osti_1458736,
title = {Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene},
author = {Seuring, Carolin and Ayyer, Kartik and Filippaki, Eleftheria and Barthelmess, Miriam and Longchamp, Jean -Nicolas and Ringler, Philippe and Pardini, Tommaso and Wojtas, David H. and Coleman, Matthew A. and Dorner, Katerina and Fuglerud, Silje and Hammarin, Greger and Habenstein, Birgit and Langkilde, Annette E. and Loquet, Antoine and Meents, Alke and Riek, Roland and Stahlberg, Henning and Boutet, Sebastien and Hunter, Mark S. and Koglin, Jason and Liang, Mengning and Ginn, Helen M. and Millane, Rick P. and Frank, Matthias and Barty, Anton and Chapman, Henry N.},
abstractNote = {Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. In conclusion, we anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.},
doi = {10.1038/s41467-018-04116-9},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {5}
}

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Cited by: 6 works
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Figures / Tables:

Fig. 1 Fig. 1: Experimental overview. a Silicon chips covered with a monolayer of graphene and a layer of fibrils were mounted in vacuum at the CXI beamline at LCLS and scanned through the XFEL focus. b Fixed-targets were made of a square silicon frame patterned with an array of 12 ×more » 13 windows. Scalebar is 5 mm. c A single window contained 81 holes of either 20 or 30 μm in diameter arrayed on a hexagonal lattice, resulting in 12,636 holes over a 2.54 × 2.54 cm area. Scalebar is 100 μm. d An atomic force microscope image of a hole covered with graphene and fibrils is shown. Fibrils were imaged next to the hole on the silicon frame, as tapping without the support destroys the graphene layer. Scalebar is 20 nm« less

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