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Title: Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. In conclusion, we anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.
Authors:
ORCiD logo [1] ; ORCiD logo [2] ;  [2] ;  [2] ; ORCiD logo [3] ;  [4] ;  [5] ;  [6] ;  [5] ;  [2] ;  [2] ; ORCiD logo [7] ;  [8] ;  [9] ; ORCiD logo [8] ; ORCiD logo [2] ;  [10] ; ORCiD logo [4] ;  [11] ;  [11] more »; ORCiD logo [11] ;  [11] ;  [12] ;  [6] ; ORCiD logo [5] ;  [2] ; ORCiD logo [13] « less
  1. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Centre for Ultrafast Imaging, Hamburg (Germany)
  2. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  3. Univ. of Zurich, Zurich (Switzerland)
  4. Univ. of Basel, Basel (Switzerland)
  5. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  6. Univ. of Canterbury, Christchurch (New Zealand)
  7. Dept. of Chemistry and Molecular Biology, Gothenburg (Sweden)
  8. Univ. de Bordeaux, Pessac (France)
  9. Univ. of Copenhagen, Copenhagen (Denmark)
  10. ETH Zurich, Zurich (Switzerland)
  11. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  12. Univ. of Oxford, Oxfordshire (United Kingdom); Diamond Light Source, Oxfordshire (United Kingdom)
  13. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); The Hamburg Centre for Ultrafast Imaging, Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Publication Date:
Grant/Contract Number:
AC02-76SF00515
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION
OSTI Identifier:
1458736

Seuring, Carolin, Ayyer, Kartik, Filippaki, Eleftheria, Barthelmess, Miriam, Longchamp, Jean -Nicolas, Ringler, Philippe, Pardini, Tommaso, Wojtas, David H., Coleman, Matthew A., Dorner, Katerina, Fuglerud, Silje, Hammarin, Greger, Habenstein, Birgit, Langkilde, Annette E., Loquet, Antoine, Meents, Alke, Riek, Roland, Stahlberg, Henning, Boutet, Sebastien, Hunter, Mark S., Koglin, Jason, Liang, Mengning, Ginn, Helen M., Millane, Rick P., Frank, Matthias, Barty, Anton, and Chapman, Henry N.. Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. United States: N. p., Web. doi:10.1038/s41467-018-04116-9.
Seuring, Carolin, Ayyer, Kartik, Filippaki, Eleftheria, Barthelmess, Miriam, Longchamp, Jean -Nicolas, Ringler, Philippe, Pardini, Tommaso, Wojtas, David H., Coleman, Matthew A., Dorner, Katerina, Fuglerud, Silje, Hammarin, Greger, Habenstein, Birgit, Langkilde, Annette E., Loquet, Antoine, Meents, Alke, Riek, Roland, Stahlberg, Henning, Boutet, Sebastien, Hunter, Mark S., Koglin, Jason, Liang, Mengning, Ginn, Helen M., Millane, Rick P., Frank, Matthias, Barty, Anton, & Chapman, Henry N.. Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene. United States. doi:10.1038/s41467-018-04116-9.
Seuring, Carolin, Ayyer, Kartik, Filippaki, Eleftheria, Barthelmess, Miriam, Longchamp, Jean -Nicolas, Ringler, Philippe, Pardini, Tommaso, Wojtas, David H., Coleman, Matthew A., Dorner, Katerina, Fuglerud, Silje, Hammarin, Greger, Habenstein, Birgit, Langkilde, Annette E., Loquet, Antoine, Meents, Alke, Riek, Roland, Stahlberg, Henning, Boutet, Sebastien, Hunter, Mark S., Koglin, Jason, Liang, Mengning, Ginn, Helen M., Millane, Rick P., Frank, Matthias, Barty, Anton, and Chapman, Henry N.. 2018. "Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene". United States. doi:10.1038/s41467-018-04116-9. https://www.osti.gov/servlets/purl/1458736.
@article{osti_1458736,
title = {Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene},
author = {Seuring, Carolin and Ayyer, Kartik and Filippaki, Eleftheria and Barthelmess, Miriam and Longchamp, Jean -Nicolas and Ringler, Philippe and Pardini, Tommaso and Wojtas, David H. and Coleman, Matthew A. and Dorner, Katerina and Fuglerud, Silje and Hammarin, Greger and Habenstein, Birgit and Langkilde, Annette E. and Loquet, Antoine and Meents, Alke and Riek, Roland and Stahlberg, Henning and Boutet, Sebastien and Hunter, Mark S. and Koglin, Jason and Liang, Mengning and Ginn, Helen M. and Millane, Rick P. and Frank, Matthias and Barty, Anton and Chapman, Henry N.},
abstractNote = {Here we present a new approach to diffraction imaging of amyloid fibrils, combining a free-standing graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 Å and 2.4 Å resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. In conclusion, we anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.},
doi = {10.1038/s41467-018-04116-9},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {2018},
month = {5}
}

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Femtosecond X-ray protein nanocrystallography
journal, February 2011
  • Chapman, Henry N.; Fromme, Petra; Barty, Anton
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  • DOI: 10.1038/nature09750

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