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Title: Structure of a symmetric photosynthetic reaction center–photosystem

Abstract

Reaction centers are pigment-protein complexes that drive photosynthesis by converting light into chemical energy. It is believed that they arose once from a homodimeric protein. The symmetry of a homodimer is broken in heterodimeric reaction center structures, such as those reported previously. The 2.2-Å resolution X-ray structure of the homodimeric reaction center/photosystem from the phototroph, Heliobacterium modesticaldum, exhibits perfect C 2 symmetry. The core polypeptide dimer and two small subunits coordinate 54 (bacterio)chlorophylls and 2 carotenoids that capture and transfer energy to the electron transfer chain at the center, which performs charge separation and consists of 6 (bacterio) chlorophylls and an iron-sulfur cluster; unlike other reaction centers, it lacks a bound quinone. Furthermore, this structure preserves characteristics of the ancestral reaction center, providing insight into evolution of photosynthesis.

Authors:
ORCiD logo [1];  [2]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [1]; ORCiD logo [4]
  1. Arizona State Univ., Tempe, AZ (United States)
  2. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  3. The Pennsylvania State Univ., University Park, PA (United States)
  4. Arizona State Univ., Tempe, AZ (United States); Biodesign Institute, Tempe, AZ (United States)
Publication Date:
Research Org.:
Arizona State Univ., Tempe, AZ (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1458602
Alternate Identifier(s):
OSTI ID: 1494447
Grant/Contract Number:  
SC0010575; award317063; award338982; award338996
Resource Type:
Published Article
Journal Name:
Science
Additional Journal Information:
Journal Volume: 357; Journal Issue: 6355; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gisriel, Christopher, Sarrou, Iosifina, Ferlez, Bryan, Golbeck, John H., Redding, Kevin E., and Fromme, Raimund. Structure of a symmetric photosynthetic reaction center–photosystem. United States: N. p., 2017. Web. doi:10.1126/science.aan5611.
Gisriel, Christopher, Sarrou, Iosifina, Ferlez, Bryan, Golbeck, John H., Redding, Kevin E., & Fromme, Raimund. Structure of a symmetric photosynthetic reaction center–photosystem. United States. doi:10.1126/science.aan5611.
Gisriel, Christopher, Sarrou, Iosifina, Ferlez, Bryan, Golbeck, John H., Redding, Kevin E., and Fromme, Raimund. Thu . "Structure of a symmetric photosynthetic reaction center–photosystem". United States. doi:10.1126/science.aan5611.
@article{osti_1458602,
title = {Structure of a symmetric photosynthetic reaction center–photosystem},
author = {Gisriel, Christopher and Sarrou, Iosifina and Ferlez, Bryan and Golbeck, John H. and Redding, Kevin E. and Fromme, Raimund},
abstractNote = {Reaction centers are pigment-protein complexes that drive photosynthesis by converting light into chemical energy. It is believed that they arose once from a homodimeric protein. The symmetry of a homodimer is broken in heterodimeric reaction center structures, such as those reported previously. The 2.2-Å resolution X-ray structure of the homodimeric reaction center/photosystem from the phototroph, Heliobacterium modesticaldum, exhibits perfect C2 symmetry. The core polypeptide dimer and two small subunits coordinate 54 (bacterio)chlorophylls and 2 carotenoids that capture and transfer energy to the electron transfer chain at the center, which performs charge separation and consists of 6 (bacterio) chlorophylls and an iron-sulfur cluster; unlike other reaction centers, it lacks a bound quinone. Furthermore, this structure preserves characteristics of the ancestral reaction center, providing insight into evolution of photosynthesis.},
doi = {10.1126/science.aan5611},
journal = {Science},
number = 6355,
volume = 357,
place = {United States},
year = {2017},
month = {7}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/science.aan5611

Citation Metrics:
Cited by: 22 works
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Figures / Tables:

Fig. 1 Fig. 1: Overall structure of the HbRC viewed from the N-side (A) or within the membrane (B). The two PshA polypeptides are colored in red and pink. PshX subunits are colored in orange. Cofactor molecules are shown as stick models and colored teal (ET), blue (antenna), and lime (carotenoids). Themore » [4Fe-4S] cluster is shown as yellow (S) and red (Fe) spheres. (B)Chl tails have been truncated for clarity.« less

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