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Title: Broad spectrum developmental role of Brachypodium AUX1

Abstract

Targeted cellular auxin distribution is required for morphogenesis and adaptive responses of plant organs. In Arabidopsis thaliana (Arabidopsis), this involves the prototypical auxin influx facilitator AUX1 and its LIKE-AUX1 (LAX) homologs, which act partially redundantly in various developmental processes. Interestingly, AUX1 and its homologs are not strictly essential for the Arabidopsis life cycle. Indeed, aux1 lax1 lax2 lax3 quadruple knock-outs are mostly viable and fertile, and strong phenotypes are only observed at low penetrance. Here we investigated the Brachypodium distachyon (Brachypodium) AUX1 homolog BdAUX1 by genetic, cell biological and physiological analyses. We report that BdAUX1 is essential for Brachypodium development. Bdaux1 loss-of-function mutants are dwarfs with aberrant flower development, and consequently infertile. Moreover, they display a counter-intuitive root phenotype. Although Bdaux1 roots are agravitropic as expected, in contrast to Arabidopsis aux1 mutants they are dramatically longer than wild type roots because of exaggerated cell elongation. Interestingly, this correlates with higher free auxin content in Bdaux1 roots. Consistently, their cell wall characteristics and transcriptome signature largely phenocopy other Brachypodium mutants with increased root auxin content. Our results imply fundamentally different wiring of auxin transport in Brachypodium roots and reveal an essential role of BdAUX1 in a broad spectrum of developmental processes,more » suggesting a central role for AUX1 in pooideae.« less

Authors:
 [1];  [2];  [3];  [4];  [3];  [2];  [1]
  1. Department of Plant Molecular Biology, University of Lausanne, Biophore Building CH-1015 Lausanne Switzerland
  2. Institute for Plant Cell Biology and Biotechnology, Heinrich-Heine University, D-40225 Duesseldorf Germany
  3. DOE Joint Genome Institute, 2800 Mitchell Dr. Walnut Creek CA 94598 USA
  4. Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83 Umeå Sweden
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1457498
Alternate Identifier(s):
OSTI ID: 1457500; OSTI ID: 1543998; OSTI ID: 1560556
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
New Phytologist
Additional Journal Information:
Journal Name: New Phytologist Journal Volume: 219 Journal Issue: 4; Journal ID: ISSN 0028-646X
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; AUX1; auxin; Brachypodium; monocotyledon; seminal root

Citation Formats

van der Schuren, Alja, Voiniciuc, Catalin, Bragg, Jennifer, Ljung, Karin, Vogel, John, Pauly, Markus, and Hardtke, Christian S. Broad spectrum developmental role of Brachypodium AUX1. United Kingdom: N. p., 2018. Web. doi:10.1111/nph.15332.
van der Schuren, Alja, Voiniciuc, Catalin, Bragg, Jennifer, Ljung, Karin, Vogel, John, Pauly, Markus, & Hardtke, Christian S. Broad spectrum developmental role of Brachypodium AUX1. United Kingdom. doi:https://doi.org/10.1111/nph.15332
van der Schuren, Alja, Voiniciuc, Catalin, Bragg, Jennifer, Ljung, Karin, Vogel, John, Pauly, Markus, and Hardtke, Christian S. Wed . "Broad spectrum developmental role of Brachypodium AUX1". United Kingdom. doi:https://doi.org/10.1111/nph.15332.
@article{osti_1457498,
title = {Broad spectrum developmental role of Brachypodium AUX1},
author = {van der Schuren, Alja and Voiniciuc, Catalin and Bragg, Jennifer and Ljung, Karin and Vogel, John and Pauly, Markus and Hardtke, Christian S.},
abstractNote = {Targeted cellular auxin distribution is required for morphogenesis and adaptive responses of plant organs. In Arabidopsis thaliana (Arabidopsis), this involves the prototypical auxin influx facilitator AUX1 and its LIKE-AUX1 (LAX) homologs, which act partially redundantly in various developmental processes. Interestingly, AUX1 and its homologs are not strictly essential for the Arabidopsis life cycle. Indeed, aux1 lax1 lax2 lax3 quadruple knock-outs are mostly viable and fertile, and strong phenotypes are only observed at low penetrance. Here we investigated the Brachypodium distachyon (Brachypodium) AUX1 homolog BdAUX1 by genetic, cell biological and physiological analyses. We report that BdAUX1 is essential for Brachypodium development. Bdaux1 loss-of-function mutants are dwarfs with aberrant flower development, and consequently infertile. Moreover, they display a counter-intuitive root phenotype. Although Bdaux1 roots are agravitropic as expected, in contrast to Arabidopsis aux1 mutants they are dramatically longer than wild type roots because of exaggerated cell elongation. Interestingly, this correlates with higher free auxin content in Bdaux1 roots. Consistently, their cell wall characteristics and transcriptome signature largely phenocopy other Brachypodium mutants with increased root auxin content. Our results imply fundamentally different wiring of auxin transport in Brachypodium roots and reveal an essential role of BdAUX1 in a broad spectrum of developmental processes, suggesting a central role for AUX1 in pooideae.},
doi = {10.1111/nph.15332},
journal = {New Phytologist},
number = 4,
volume = 219,
place = {United Kingdom},
year = {2018},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
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DOI: https://doi.org/10.1111/nph.15332

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Cited by: 8 works
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    journal, August 2019


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    journal, November 2018

    • Buendia, Luis; Maillet, Fabienne; O'Connor, Devin
    • New Phytologist, Vol. 221, Issue 4
    • DOI: 10.1111/nph.15551

    Arabidopsis and sunflower plants with increased xylem area show enhanced seed yield
    journal, May 2019

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    • DOI: 10.1111/tpj.14356

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    • DOI: 10.1242/dev.172965

    Developmental Roles of AUX1/LAX Auxin Influx Carriers in Plants
    journal, October 2019


    In Vitro Tissue Culture in Brachypodium: Applications and Challenges
    journal, February 2020

    • Betekhtin, Alexander; Hus, Karolina; Rojek-Jelonek, Magdalena
    • International Journal of Molecular Sciences, Vol. 21, Issue 3
    • DOI: 10.3390/ijms21031037