Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

Denby, Charles M.; Li, Rachel A.; Vu, Van T.; Costello, Zak; Lin, Weiyin; Chan, Leanne Jade G.; Williams, Joseph; Donaldson, Bryan; Bamforth, Charles W.; Petzold, Christopher J.; Scheller, Henrik V.; Martin, Hector Garcia; Keasling, Jay D.

doi:10.1038/s41467-018-03293-x

Title: Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

Journal Article · 20 March 2018 · Nature Communications

DOI: https://doi.org/10.1038/s41467-018-03293-x · OSTI ID:1457017

Denby, Charles M. ^[1]; Li, Rachel A. ^[2]; Vu, Van T. ^[3]; Costello, Zak ^[4]; Lin, Weiyin ^[1]; Chan, Leanne Jade G. ^[5]; Williams, Joseph ^[6]; Donaldson, Bryan ^[7];

^[6]; Petzold, Christopher J. ^[5]; Scheller, Henrik V. ^[8];

^[4];

^[9]

Univ. of California, Berkeley, CA (United States). California Inst. of Quantitative Biosciences (QB3); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; DOE Agile BioFoundry, Emeryville, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
Univ. of California, Davis, CA (United States). Dept. of Food Science and Technology
Lagunitas Brewing Company, Petaluma, CA (United States)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology Division
Univ. of California, Berkeley, CA (United States). California Inst. of Quantitative Biosciences (QB3); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering; Univ. of California, Berkeley, CA (United States). Dept. of Chemical and Biomolecular Engineering; Technical Univ. of Denmark, Hellerup (Denmark). Novo Nordisk Foundation Center for Sustainability

Flowers of the hop plant provide both bitterness and "hoppy" flavor to beer. Hops are, however, both a water and energy intensive crop and vary considerably in essential oil content, making it challenging to achieve a consistent hoppy taste in beer. Here, we report that brewer's yeast can be engineered to biosynthesize aromatic monoterpene molecules that impart hoppy flavor to beer by incorporating recombinant DNA derived from yeast, mint, and basil. Whereas metabolic engineering of biosynthetic pathways is commonly enlisted to maximize product titers, tuning expression of pathway enzymes to affect target production levels of multiple commercially important metabolites without major collateral metabolic changes represents a unique challenge. By applying state-of-the-art engineering techniques and a framework to guide iterative improvement, strains are generated with target performance characteristics. Finally, beers produced using these strains are perceived as hoppier than traditionally hopped beers by a sensory panel in a double-blind tasting.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; 1722376

OSTI ID:: 1457017

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Functional optimization of gene clusters by combinatorial design and assembly Smanski, Michael J.; Bhatia, Swapnil; Zhao, Dehua Nature Biotechnology, Vol. 32, Issue 12, p. 1241-1249 https://doi.org/10.1038/nbt.3063	journal	November 2014
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