Guanidinium Group Remains Protonated in a Strongly Basic Arginine Solution
Abstract
Knowledge of the acid dissociation constant of an amino acid has very important ramifications in the biochemistry of proteins and lipid bilayers in aqueous environments because charge and proton transfer depend on its value. The acid dissociation constant for the guanidinium group in arginine has historically been posited as 12.5, but there is substantial variation in published values over the years. Recent experiments suggest that the dissociation constant for arginine is much higher than 12.5, which explains why the arginine guanidinium group retains its positive charge under all physiological conditions. Here, we use X-ray photoelectron spectroscopy to study unsupported, aqueous arginine nanoparticles. By varying the pH of the constituent solution, we provide evidence that the guanidinium group is protonated even in a very basic solution. By analyzing the energy shifts in the C and N X-ray photoelectron spectra, we establish a molecular level picture of how charge and proton transport in aqueous solutions of arginine occur.
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Chemical Sciences Division
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Chemical Sciences Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Science Foundation (NSF)
- OSTI Identifier:
- 1454493
- Alternate Identifier(s):
- OSTI ID: 1401071
- Grant/Contract Number:
- AC02-05CH11231; DGE‐1106400
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ChemPhysChem
- Additional Journal Information:
- Journal Volume: 18; Journal Issue: 12; Related Information: © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim; Journal ID: ISSN 1439-4235
- Publisher:
- ChemPubSoc Europe
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; acid dissociation constant; amino acids; nanoparticles; photoelectron spectroscopy; proton transport
Citation Formats
Xu, Bo, Jacobs, Michael I., Kostko, Oleg, and Ahmed, Musahid. Guanidinium Group Remains Protonated in a Strongly Basic Arginine Solution. United States: N. p., 2017.
Web. doi:10.1002/cphc.201700197.
Xu, Bo, Jacobs, Michael I., Kostko, Oleg, & Ahmed, Musahid. Guanidinium Group Remains Protonated in a Strongly Basic Arginine Solution. United States. https://doi.org/10.1002/cphc.201700197
Xu, Bo, Jacobs, Michael I., Kostko, Oleg, and Ahmed, Musahid. Tue .
"Guanidinium Group Remains Protonated in a Strongly Basic Arginine Solution". United States. https://doi.org/10.1002/cphc.201700197. https://www.osti.gov/servlets/purl/1454493.
@article{osti_1454493,
title = {Guanidinium Group Remains Protonated in a Strongly Basic Arginine Solution},
author = {Xu, Bo and Jacobs, Michael I. and Kostko, Oleg and Ahmed, Musahid},
abstractNote = {Knowledge of the acid dissociation constant of an amino acid has very important ramifications in the biochemistry of proteins and lipid bilayers in aqueous environments because charge and proton transfer depend on its value. The acid dissociation constant for the guanidinium group in arginine has historically been posited as 12.5, but there is substantial variation in published values over the years. Recent experiments suggest that the dissociation constant for arginine is much higher than 12.5, which explains why the arginine guanidinium group retains its positive charge under all physiological conditions. Here, we use X-ray photoelectron spectroscopy to study unsupported, aqueous arginine nanoparticles. By varying the pH of the constituent solution, we provide evidence that the guanidinium group is protonated even in a very basic solution. By analyzing the energy shifts in the C and N X-ray photoelectron spectra, we establish a molecular level picture of how charge and proton transport in aqueous solutions of arginine occur.},
doi = {10.1002/cphc.201700197},
journal = {ChemPhysChem},
number = 12,
volume = 18,
place = {United States},
year = {Tue May 16 00:00:00 EDT 2017},
month = {Tue May 16 00:00:00 EDT 2017}
}
Web of Science
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