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Title: Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange

In this paper, the three-dimensional spatial distributions of a number of metabolites involved in regulating symbiosis and biological nitrogen fixation (BNF) within soybean root nodules were revealed using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). While many metabolites exhibited distinct spatial compartmentalization, some metabolites were asymmetrically distributed throughout the nodule (e.g., S-adenosylmethionine). These results establish a more complex metabolic view of plant–bacteria symbiosis (and BNF) within soybean nodules than previously hypothesized. Collectively these findings suggest that spatial perspectives in metabolic regulation should be considered to unravel the overall complexity of interacting organisms, like those relating to associations of nitrogen-fixing bacteria with host plants.
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [1] ;  [1] ;  [2] ; ORCiD logo [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  2. Univ. of Missouri, Columbia, MO (United States)
  3. The George Washington Univ., Washington, D.C. (United States)
Publication Date:
Report Number(s):
PNNL-SA-127419
Journal ID: ISSN 1751-7362; PII: 188
Grant/Contract Number:
DOE-FOA-0001192; 1734145; AC05-76RL01830
Type:
Published Article
Journal Name:
The ISME Journal
Additional Journal Information:
Journal Volume: 12; Journal ID: ISSN 1751-7362
Publisher:
Nature Publishing Group
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1454346
Alternate Identifier(s):
OSTI ID: 1458774

Velicckovic, Dusan, Agtuca, Beverly J., Stopka, Sylwia A., Vertes, Akos, Koppenaal, David W., Pasa-Tolic, Ljiljana, Stacey, Gary, and Anderton, Christopher R.. Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange. United States: N. p., Web. doi:10.1038/s41396-018-0188-8.
Velicckovic, Dusan, Agtuca, Beverly J., Stopka, Sylwia A., Vertes, Akos, Koppenaal, David W., Pasa-Tolic, Ljiljana, Stacey, Gary, & Anderton, Christopher R.. Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange. United States. doi:10.1038/s41396-018-0188-8.
Velicckovic, Dusan, Agtuca, Beverly J., Stopka, Sylwia A., Vertes, Akos, Koppenaal, David W., Pasa-Tolic, Ljiljana, Stacey, Gary, and Anderton, Christopher R.. 2018. "Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange". United States. doi:10.1038/s41396-018-0188-8.
@article{osti_1454346,
title = {Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange},
author = {Velicckovic, Dusan and Agtuca, Beverly J. and Stopka, Sylwia A. and Vertes, Akos and Koppenaal, David W. and Pasa-Tolic, Ljiljana and Stacey, Gary and Anderton, Christopher R.},
abstractNote = {In this paper, the three-dimensional spatial distributions of a number of metabolites involved in regulating symbiosis and biological nitrogen fixation (BNF) within soybean root nodules were revealed using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). While many metabolites exhibited distinct spatial compartmentalization, some metabolites were asymmetrically distributed throughout the nodule (e.g., S-adenosylmethionine). These results establish a more complex metabolic view of plant–bacteria symbiosis (and BNF) within soybean nodules than previously hypothesized. Collectively these findings suggest that spatial perspectives in metabolic regulation should be considered to unravel the overall complexity of interacting organisms, like those relating to associations of nitrogen-fixing bacteria with host plants.},
doi = {10.1038/s41396-018-0188-8},
journal = {The ISME Journal},
number = ,
volume = 12,
place = {United States},
year = {2018},
month = {6}
}