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Title: SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus

The development of more accurate and sensitive diagnostic techniques is a key factor in efforts to improve cancer survival rates. The technique of infrared aperture fibre scanning near-field optical microscopy (IR-SNOM),together with radiation from the infrared free-electron laser (IR-FEL) on ALICEat Daresbury Laboratory (UK), has been used to obtain IR images of a crypt-like feature and the surrounding tissue; the tissue was taken from a patient with oesophageal adenocarcinoma and with a history of Barrett’s oesophagus. We have shown that the DNA signal is enhanced relative to other contributions in the region of the crypt, and the glycoprotein signal shows a less pronounced increase in the region of the crypt. The Amide II signal is found to be anti-correlated with the DNA and glycoprotein profiles. The absorbance of the Amide II signal is found to differ for three different types of cancer tissue. In conclusion, high-resolution IR images of the crypt reveal additional structure that would not be resolved in diffraction-limited techniques.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [3] ;  [4] ;  [4] ;  [5] ;  [3] ;  [1] ; ORCiD logo [2]
  1. Univ. of Liverpool, Liverpool (United Kingdom)
  2. Univ. of Liverpool, Liverpool (United Kingdom); Cockcroft Institute Sci-Tech Daresbury, Warrington (United Kingdom); Accelerator Science and Technology Centre (ASTeC) STFC Daresbury Lab. Sci-Tech Daresbury, Warrington (United Kingdom)
  3. Univ. of Liverpool, Liverpool (United Kingdom); Cockcroft Institute Sci-Tech Daresbury, Warrington (United Kingdom)
  4. Istituto di Struttura della Materia, Rome (Italy)
  5. Cockcroft Institute Sci-Tech Daresbury, Warrington (United Kingdom); Accelerator Science and Technology Centre (ASTeC) STFC Daresbury Lab. Sci-Tech Daresbury, Warrington (United Kingdom)
Publication Date:
Report Number(s):
FERMILAB-PUB-18-242
Journal ID: ISSN 0370-1972; 1677477
Grant/Contract Number:
AC02-07CH11359
Type:
Accepted Manuscript
Journal Name:
Physica Status Solidi B. Basic Solid State Physics
Additional Journal Information:
Journal Volume: 255; Journal Issue: 4; Journal ID: ISSN 0370-1972
Publisher:
Wiley-Blackwell
Research Org:
Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)
Sponsoring Org:
USDOE Office of Science (SC), High Energy Physics (HEP) (SC-25)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; adenocarcinoma; DNA; infrared radiation; scanning near-field optical microscopy; tissue
OSTI Identifier:
1452785