skip to main content

DOE PAGESDOE PAGES

This content will become publicly available on May 25, 2019

Title: Short-term transcriptional response of microbial communities to N-fertilization in pine forest soil

Numerous studies have examined the long-term effect of experimental nitrogen (N) deposition in terrestrial ecosystems, however N-specific mechanistic markers are difficult to disentangle from responses to other environmental changes. The strongest picture of N-responsive mechanistic markers is likely to arise from measurements over a short (hours to days) timescale immediately after inorganic N deposition. Therefore, we assessed the short-term (3-day) transcriptional response of microbial communities in two soil strata from a pine forest to a high dose of N fertilization (c.a. 1mg/g of soil material) in laboratory microcosms. Here, we hypothesized that N fertilization would repress the expression of fungal and bacterial genes linked to N-mining from plant litter. However, despite N-suppression of microbial respiration, the most pronounced differences in functional gene expression were between strata rather than in response to the N addition. Overall, ~4% of metabolic genes changed in expression with N addition, while three times as many (~12%) were significantly different across the different soil strata in the microcosms. In particular, we found little evidence of N changing expression levels of metabolic genes associated with complex carbohydrate degradation (CAZymes) or inorganic N utilization. This suggests that direct N repression of microbial functional gene expression is not themore » principle mechanism for reduced soil respiration immediately after N deposition. Instead, changes in expression with N addition occurred primarily in general cell maintenance areas, for example in ribosome-related transcripts. Transcriptional changes in functional gene abundance in response to N-addition observed in longer-term field studies likely results from changes in microbial composition.« less
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ; ORCiD logo [1] ; ORCiD logo [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. Connecticut Agricultural Experiment Station, New Haven, CT (United States). Dept. of Environmental Sciences
Publication Date:
Report Number(s):
LA-UR-18-21874
Journal ID: ISSN 0099-2240; 1098-5336 (Electronic)
Grant/Contract Number:
AC52-06NA25396
Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 84; Journal Issue: 15; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Research Org:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; metatranscriptomics; nitrogen fertilization; microbial community; carbohydrate-active enzymes
OSTI Identifier:
1441353

Albright, Michaeline Burr Nelson, Johansen, Renee, Lopez, Deanna, Gallegos-Graves, Laverne A, Steven, Blaire, Kuske, Cheryl R., and Dunbar, John. Short-term transcriptional response of microbial communities to N-fertilization in pine forest soil. United States: N. p., Web. doi:10.1128/AEM.00598-18.
Albright, Michaeline Burr Nelson, Johansen, Renee, Lopez, Deanna, Gallegos-Graves, Laverne A, Steven, Blaire, Kuske, Cheryl R., & Dunbar, John. Short-term transcriptional response of microbial communities to N-fertilization in pine forest soil. United States. doi:10.1128/AEM.00598-18.
Albright, Michaeline Burr Nelson, Johansen, Renee, Lopez, Deanna, Gallegos-Graves, Laverne A, Steven, Blaire, Kuske, Cheryl R., and Dunbar, John. 2018. "Short-term transcriptional response of microbial communities to N-fertilization in pine forest soil". United States. doi:10.1128/AEM.00598-18.
@article{osti_1441353,
title = {Short-term transcriptional response of microbial communities to N-fertilization in pine forest soil},
author = {Albright, Michaeline Burr Nelson and Johansen, Renee and Lopez, Deanna and Gallegos-Graves, Laverne A and Steven, Blaire and Kuske, Cheryl R. and Dunbar, John},
abstractNote = {Numerous studies have examined the long-term effect of experimental nitrogen (N) deposition in terrestrial ecosystems, however N-specific mechanistic markers are difficult to disentangle from responses to other environmental changes. The strongest picture of N-responsive mechanistic markers is likely to arise from measurements over a short (hours to days) timescale immediately after inorganic N deposition. Therefore, we assessed the short-term (3-day) transcriptional response of microbial communities in two soil strata from a pine forest to a high dose of N fertilization (c.a. 1mg/g of soil material) in laboratory microcosms. Here, we hypothesized that N fertilization would repress the expression of fungal and bacterial genes linked to N-mining from plant litter. However, despite N-suppression of microbial respiration, the most pronounced differences in functional gene expression were between strata rather than in response to the N addition. Overall, ~4% of metabolic genes changed in expression with N addition, while three times as many (~12%) were significantly different across the different soil strata in the microcosms. In particular, we found little evidence of N changing expression levels of metabolic genes associated with complex carbohydrate degradation (CAZymes) or inorganic N utilization. This suggests that direct N repression of microbial functional gene expression is not the principle mechanism for reduced soil respiration immediately after N deposition. Instead, changes in expression with N addition occurred primarily in general cell maintenance areas, for example in ribosome-related transcripts. Transcriptional changes in functional gene abundance in response to N-addition observed in longer-term field studies likely results from changes in microbial composition.},
doi = {10.1128/AEM.00598-18},
journal = {Applied and Environmental Microbiology},
number = 15,
volume = 84,
place = {United States},
year = {2018},
month = {5}
}