Transcriptomic analysis of skin pigmentation variation in the Virginia opossum ( Didelphis virginiana )

Nigenda‐Morales, Sergio F.; Hu, Yibo; Beasley, James C.; Ruiz‐Piña, Hugo A.; Valenzuela‐Galván, David; Wayne, Robert K.

doi:10.1111/mec.14712

Title: Transcriptomic analysis of skin pigmentation variation in the Virginia opossum ( Didelphis virginiana )

Journal Article · 03 June 2018 · Molecular Ecology

DOI: https://doi.org/10.1111/mec.14712 · OSTI ID:1440263

^[1]; Hu, Yibo ^[2]; Beasley, James C. ^[3]; Ruiz‐Piña, Hugo A. ^[4]; Valenzuela‐Galván, David ^[5]; Wayne, Robert K. ^[1]

Department of Ecology and Evolutionary Biology University of California, Los Angeles Los Angeles California
Key Lab of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Chaoyang, Beijing China
Savannah River Ecology Lab Warnell School of Forestry and Natural Resources University of Georgia Aiken South Carolina
Centro de Investigaciones Regionales “Dr. Hideyo Noguchi” Universidad Autónoma de Yucatán Mérida Yucatán Mexico
Departamento de Ecología Evolutiva Centro de Investigación en Biodiversidad y Conservación Universidad Autónoma del Estado de Morelos Cuernavaca Morelos Mexico

Abstract Skin pigmentation and coat pigmentation are two of the best‐studied examples of traits under natural selection given their quantifiable fitness interactions with the environment (e.g., camouflage) and signalling with other organisms (e.g., warning coloration). Previous morphological studies have found that skin pigmentation variation in the Virginia opossum ( Didelphis virginiana ) is associated with variation in precipitation and temperatures across its distribution range following Gloger's rule (lighter pigmentation in temperate environments). To investigate the molecular mechanism associated with skin pigmentation variation, we used RNA‐Seq and quantified gene expression of wild opossums from tropical and temperate populations. Using differential expression analysis and a co‐expression network approach, we found that expression variation in genes with melanocytic and immune functions is significantly associated with the degree of skin pigmentation variation and may be underlying this phenotypic difference. We also found evidence suggesting that the Wnt/β‐catenin signalling pathway might be regulating the depigmentation observed in temperate populations. Based on our study results, we present several alternative hypotheses that may explain Gloger's rule pattern of skin pigmentation variation in opossum, including changes in pathogen diversity supporting a pathogen‐resistant hypothesis, thermal stress associated with temperate environments, and pleiotropic and epistatic interactions between melanocytic and immune genes.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FC09-07SR22506

OSTI ID:: 1440263

Journal Information:: Molecular Ecology, Journal Name: Molecular Ecology Journal Issue: 12 Vol. 27; ISSN 0962-1083

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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