skip to main content

DOE PAGESDOE PAGES

Title: Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants

Here, we examined the effect of downregulating PdKOR1 gene, an endo-β-1,4-glucanase gene family member previously characterized to affect cellulose biosynthesis and cell wall composition in Populus, on the secondary metabolome and microbiome of field-grown Populus deltoides. We revealed differences in metabolite profiles of PdKOR1 RNAi and control roots using gas chromatography-mass spectrometry, and microbiome identification via Illumina MiSeq 16S and ITS2 rRNA sequencing in root endospheres and rhizospheres. PdKOR1 RNAi root metabolites differed from control plants: free amino acids (valine, isoleucine, alanine) were reduced while caffeoyl-shikimates, salicylic-acid derivatives, and flavonoid metabolites increased in PdKOR1 RNAi roots. The Actinobacterial family Micromonosporaceae were more abundant in RNAi root endospheres, whereas Nitrospirae was reduced in PdKOR1 RNAi rhizospheres. Ascomycota were lower and Basidiomycota greater in PdKOR1 rhizospheres. Bacterial and fungal community composition, as measured by Bray-Curtis dissimilarity, differed between PdKOR1 RNAi and control rhizospheres and endospheres. In conclusion, these results indicate that modification of plant cell walls via downregulation of PdKOR1 gene in Populus impacts carbon metabolism in roots and concomitant alterations in root-associated microbial communities. Such an understanding of functional and ecological implications of biomass chemistry improvement efforts is critical to address the goals of sustainable bioenergy crop production and management.
Authors:
ORCiD logo [1] ;  [1] ;  [1] ;  [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [1]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Published Article
Journal Name:
Plant and Soil
Additional Journal Information:
Journal Volume: 429; Journal Issue: 1-2; Journal ID: ISSN 0032-079X
Publisher:
Springer
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
Cellulose; Cell wall alteration; Endo-1,4-ß-glucanase; Microbiome; Metabolome; Populus
OSTI Identifier:
1440261
Alternate Identifier(s):
OSTI ID: 1462903

Veach, Allison M., Yip, Daniel Z., Engle, Nancy L., Yang, Zamin Koo, Bible, Amber N., Morrell-Falvey, Jennifer L., Tschaplinski, Timothy J., Kalluri, Udaya C., and Schadt, Christopher Warren. Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants. United States: N. p., Web. doi:10.1007/s11104-018-3692-8.
Veach, Allison M., Yip, Daniel Z., Engle, Nancy L., Yang, Zamin Koo, Bible, Amber N., Morrell-Falvey, Jennifer L., Tschaplinski, Timothy J., Kalluri, Udaya C., & Schadt, Christopher Warren. Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants. United States. doi:10.1007/s11104-018-3692-8.
Veach, Allison M., Yip, Daniel Z., Engle, Nancy L., Yang, Zamin Koo, Bible, Amber N., Morrell-Falvey, Jennifer L., Tschaplinski, Timothy J., Kalluri, Udaya C., and Schadt, Christopher Warren. 2018. "Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants". United States. doi:10.1007/s11104-018-3692-8.
@article{osti_1440261,
title = {Modification of plant cell wall chemistry impacts metabolome and microbiome composition in Populus PdKOR1 RNAi plants},
author = {Veach, Allison M. and Yip, Daniel Z. and Engle, Nancy L. and Yang, Zamin Koo and Bible, Amber N. and Morrell-Falvey, Jennifer L. and Tschaplinski, Timothy J. and Kalluri, Udaya C. and Schadt, Christopher Warren},
abstractNote = {Here, we examined the effect of downregulating PdKOR1 gene, an endo-β-1,4-glucanase gene family member previously characterized to affect cellulose biosynthesis and cell wall composition in Populus, on the secondary metabolome and microbiome of field-grown Populus deltoides. We revealed differences in metabolite profiles of PdKOR1 RNAi and control roots using gas chromatography-mass spectrometry, and microbiome identification via Illumina MiSeq 16S and ITS2 rRNA sequencing in root endospheres and rhizospheres. PdKOR1 RNAi root metabolites differed from control plants: free amino acids (valine, isoleucine, alanine) were reduced while caffeoyl-shikimates, salicylic-acid derivatives, and flavonoid metabolites increased in PdKOR1 RNAi roots. The Actinobacterial family Micromonosporaceae were more abundant in RNAi root endospheres, whereas Nitrospirae was reduced in PdKOR1 RNAi rhizospheres. Ascomycota were lower and Basidiomycota greater in PdKOR1 rhizospheres. Bacterial and fungal community composition, as measured by Bray-Curtis dissimilarity, differed between PdKOR1 RNAi and control rhizospheres and endospheres. In conclusion, these results indicate that modification of plant cell walls via downregulation of PdKOR1 gene in Populus impacts carbon metabolism in roots and concomitant alterations in root-associated microbial communities. Such an understanding of functional and ecological implications of biomass chemistry improvement efforts is critical to address the goals of sustainable bioenergy crop production and management.},
doi = {10.1007/s11104-018-3692-8},
journal = {Plant and Soil},
number = 1-2,
volume = 429,
place = {United States},
year = {2018},
month = {6}
}

Works referenced in this record:

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011
  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Engineering of plants with improved properties as biofuels feedstocks by vessel-specific complementation of xylan biosynthesis mutants
journal, January 2012
  • Petersen, Pia Damm; Lau, Jane; Ebert, Berit
  • Biotechnology for Biofuels, Vol. 5, Issue 1, Article No. 84
  • DOI: 10.1186/1754-6834-5-84