Title: Hyperspectral reflectance as a tool to measure biochemical and physiological traits in wheat

Abstract

Improving photosynthesis to raise wheat yield potential has emerged as a major target for wheat physiologists. Photosynthesis-related traits, such as nitrogen per unit leaf area (N_area) and leaf dry mass per area (LMA), require laborious, destructive, laboratory-based methods, while physiological traits underpinning photosynthetic capacity, such as maximum Rubisco activity normalized to 25 °C (V_cmax25) and electron transport rate (J), require time-consuming gas exchange measurements. The aim of this study was to assess whether hyperspectral reflectance (350–2500 nm) can be used to rapidly estimate these traits on intact wheat leaves. Predictive models were constructed using gas exchange and hyperspectral reflectance data from 76 genotypes grown in glasshouses with different nitrogen levels and/or in the field under yield potential conditions. Models were developed using half of the observed data with the remainder used for validation, yielding correlation coefficients (R² values) of 0.62 for V_cmax25, 0.7 for J, 0.81 for SPAD, 0.89 for LMA, and 0.93 for N_area, with bias <0.7%. The models were tested on elite lines and landraces that had not been used to create the models. The bias varied between -2.3% and -5.5% while relative error of prediction was similar for SPAD but slightly greater for LMA and N_area.

Authors:

Silva-Perez, Viridiana  ^[1];

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Molero, Gemma  ^[2];

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Serbin, Shawn P.  ^[3];

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Condon, Anthony G. ^[1]; Reynolds, Matthew P.  ^[2];

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Furbank, Robert T.  ^[1];

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Evans, John R.  ^[4]

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+ Show Author Affiliations

1. CSIRO Agriculture, Canberra, ACT (Australia); Australian National Univ., Canberra, ACT (Australia). ARC Centre of Excellence for Translational Photosynthesis. Research School of Biology
2. International Maize and Wheat Improvement Centre (CIMMYT), Mexico City (Mexico)
3. Brookhaven National Lab. (BNL), Upton, NY (United States). Environmental and Climate Sciences Dept.
4. Australian National Univ., Canberra, ACT (Australia). ARC Centre of Excellence for Translational Photosynthesis. Research School of Biology

Publication Date:

2017-12-22

Research Org.:

Brookhaven National Lab. (BNL), Upton, NY (United States); International Maize and Wheat Improvement Centre (CIMMYT), Mexico City (Mexico); CSIRO Agriculture, Canberra, ACT (Australia); Australian National Univ., Canberra, ACT (Australia)

Sponsoring Org.:

USDOE; Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA) (Mexico); National Council of Science and Technology (CONACYT) (Mexico); Australian Research Council (ARC); Grains Research & Development Corporation (Australia)

OSTI Identifier:

1439796

Report Number(s):

BNL-114530-2017-JAAM
Journal ID: ISSN 0022-0957

Grant/Contract Number:  

SC0012704; 207607; CE140100015; CSP00168

Resource Type:

Accepted Manuscript

Journal Name:

Journal of Experimental Botany

Additional Journal Information:

Journal Volume: 69; Journal Issue: 3; Journal ID: ISSN 0022-0957

Publisher:

Oxford University Press

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; electron transport rate; hyperspectral reflectance; leaf dry mass per area; leaf nitrogen; partial least squares; photosynthesis; Rubisco; Triticum aestivum; velocity of carboxylation